A transfected c-myc oncogene inhibits mouse erythroleukemic differentiation.
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E Dmitrovsky
Publications and source records attributed to E Dmitrovsky.
Lymph node aspiration in the management of Hodgkin's disease.
Diagnosing recurrent Hodgkin's disease is an important oncologic problem. When relapse does not occur in peripheral nodal sites, then a major surgical exploration is often considered. Lymph node aspiration is proposed as a less invasive approach capable of establishing a diagnosis in some instances. In this retrospective study, 19 patients with Hodgkin's disease underwent 64 lymph node aspirations. Of these patients, 17 had suspected Hodgkin's disease with involvement at an inaccessible site. Two patients with a primary diagnosis of Hodgkin's disease underwent aspiration of a peripheral site. Of these 19 patients, five (26.3%) had a positive aspirate and only two (10.5%) had an unsatisfactory aspirate. No patient had a false-positive aspirate. Of 15 patients with a negative aspirate, five (33%) had a false-negative aspirate. In no case did a false-negative aspirate delay appropriate therapy. In those patients with a positive aspirate, surgical exploration was avoided. We conclude that lymph node aspiration cytology is useful as an initial step to document clinical relapse of Hodgkin's disease at a site that might require a major surgical procedure for diagnosis.
Adult↗
Calcium-acidic phospholipid-phosphate complexes in human atherosclerotic aortas.
Since aortic calcification chemically resembles bone mineralization, we tested the hypothesis that a bone mineral nucleator, the Ca-acidic phospholipid-phosphate complexes, is found in atherosclerotic plaques. Calcium-acidic phospholipid-phosphate complexes were isolated from hydroxyapatite-containing calcified plaques and from adjacent nonmineralized areas of adult human aortas. Neonatal aortas, which served as nonmineralized lesion-free controls, contained negligible amounts of the Ca-acidic phospholipid-phosphate complexes. The concentration of complexed acidic phospholipids in the aortic plaques (5 micrograms/mg demineralized dry wt) was comparable to that found in newly mineralized bone and calcified cartilage. The presence of Ca-acidic phospholipid-phosphate complexes in the nonmineralized regions of the adult aorta, as well as in the mineralized plaques, suggests that this tissue may calcify through mechanisms similar to those involved in bone mineralization.
Adult↗
Expression of a transfected human c-myc oncogene inhibits differentiation of a mouse erythroleukaemia cell line.
The Friend-virus-derived mouse erythroleukaemia (MEL) cell lines represent transformed early erythroid precursors that can be induced to differentiate into more mature erythroid cells by a variety of agents including dimethyl sulphoxide (DMSO). There is a latent period of 12 hours after inducer is added, when 80-90% of the cells become irreversibly committed to the differentiation programme, undergoing several rounds of cell division before permanently ceasing to replicate. After DMSO induction, a biphasic decline in steady-state levels of c-myc and c-myb messenger RNAs occurs. Following the initial decrease in c-myc mRNA expression, the subsequent increase occurs in, and is restricted to, the G1 phase of the cell cycle. We sought to determine whether the down-regulation is a necessary step in chemically induced differentiation. Experiments reported here indicate that expression in MEL cells of a transfected human c-myc gene inhibits the terminal differentiation process.
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