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Biomedical subjects

E Dolfini

Publications and source records attributed to E Dolfini.

At least 37 records · Page 2Linked to original sources
PubMed1980

Preservation of rat hepatic microsomal enzyme activities: effect of low temperature and freeze-drying.

About 30-70% of microsomal hydroxylation of aniline, 0-demethylation of 4-NO2-anisole, N-demethylation of aminopyrine, were lost when whole organs were frozen and kept at low temperatures (0 degrees, -20 degrees, -196 degrees C). When 9000 X g or 105000 X g fractions were prepared from fresh liver and subsequently frozen to different temperatures, there was little or no such loss of activity. The kinetics of the decrease in microsomal enzyme activities was followed during storage of frozen or freeze-dried microsomes at various temperatures. N-demethylation of aminopyrine appeared to be the most sensitive marker of microsome denaturation.

Aminopyrine↗
PubMed1977

Tolerance to the increase of striatal homovanillic acid elicited by several anorectic drugs.

Several anorectic drugs affect dopamine metabolism in the rat striatum, increasing the concentration of homovanillic acid (HVA). However 1- and d-amphetamine and mazindol develop a tolerance to the HVA increase. On the contrary, the effect of fenfluramine and S 992 is not reduced by sub-chronic treatments. Moreover, a cross-tolerance to this biochemical effect develops between amphetamine and mazindol, but pretreatment with fenfluramine or with S 992 does not induce cross-tolerance to amphetamine.

Animals↗
PubMed1976

Synthesis and anticancer activity of 5-diethylaminomethyl derivatives and nitrogen mustards of uracil and 2-thiouracils.

Several 5-diethylaminomethyl derivatives and nitrogen mustards of uracil and 2-thiouracil have been synthesized and tested for their potential anticancer activity in vitro on KB cells and in vivo on Ehrlich carcinoma. Among the alkylating derivatives tested several showed cytotoxic activity in vitro and compound V [5-[bis(2-chloroethyl) amino] methyl-6-propyluracil hydrochloride] showed both in vitro and in vivo anticancer activity.

Alkylating Agents↗
PubMed1976

Modifications of striatal dopamine levels by steroid contraceptive drugs in mice and rats.

The effect of steroid contraceptive drugs (SCDs) on the concentration of dopamine (DA) in the striatum was studied in mice and rats. Mestranol was combined with each one of the three progestins, lynestrenol, norethindrone and norethynodrel. Treatments were given daily for 1, 4 or 30 days; animals were sacrificed 1 or 18 h after the last administration. In all groups, with the exception of the animals sacrificed 1 h after an acute treatment, striatal DA was significantly lower than in controls.

Animals↗
PubMed1976

Modifications of the striatal dopamine metabolism during the estrus cycle in mice.

The metabolism of striatum dopamine (DA) was studied in mice during proestrus, estrus and diestrus. The following results have been obtained: (1) the concentration of DA is higher in diestrus than in proestrus and estrus; (2) the concentration of its metabolite, homovanillic acid (HVA), is maximum in the estrus phase; (3) the DA turnover, evaluated by measuring the DA disappearance after blocking synthesis with methyltyrosine, is faster in estrus than in the other phases. These results have been discussed in view of the possible role of dopaminergic mechanisms in the control of gonadotropin secretion.

Animals↗
PubMed1976

Biochemical effects of treatment with oral contraceptive steroids on the dopaminergic system of the rat.

The effects of acute and chronic administration of a combination of lynestrenol-mestrenol, a widely employed contraceptive medication, on the dopaminergic system of the rat forebrain and striatum were investigated to better understand the biochemical basis of the neurological side-effects of steroid contraceptive drugs (SCDs). Both acute and chronic treatment increased the disappearance rate of striatal dopamine (DA) after synthesis blockade with alpha-methyl-p-tyrosine (alpha-MpT). Moreover, the conversion of 3H-tyrosine (3H-T) into 3H-DA was increased in the forebrain and striatum after chronic administration of this steroid combination. In the same animals, the utilization of tyrosine (T) is increased.

Animals↗
PubMed1975

Effect of ECT and imipramine treatment on the concentration of 5-hydroxyindoleacetic acid (5HIAA) and homovanillic acid (HVA) in the cerebrospinal fluid of depressed patients.

The influence of probenecid administration on 5HIAA and HVA concentrations in the CSF of depressed patients, was studied before and after treatment with imipramine or ECT. The average increase of the two metabolites in the CSF after probenecid was similar in the untreated depressed patients and in the same patients improved after both imipramine or ECT treatment. The treatment determined a significant increase in the CSF concentration of the acid metabolites also before the probenecid administration.

Adult↗