Biomedical subjects

E Dy

Publications and source records attributed to E Dy.

3 recordsLinked to original sources

PubMed2001

RT-PCR reveals muscarinic acetylcholine receptor mRNA in the pre-Bötzinger complex.

Muscarinic receptors mediate the postsynaptic excitatory effects of acetylcholine (ACh) on inspiratory neurons in the pre-Bötzinger complex (pre-BötC), the hypothesized site for respiratory rhythm generation. Because pharmacological tools for identifying the subtypes of the muscarinic receptors that underlie these effects are limited, we probed for mRNA for these receptors in the pre-BötC. We used RT-PCR to determine the expression of muscarinic receptor subtypes in tissue punches of the pre-BötC taken from rat medullary slices. Cholinergic receptor subtype M(2) and M(3) mRNAs were observed in the first round of PCR amplification. All five subtypes, M(1)-M(5), were observed in the second round of amplification. Our results suggest that the majority of muscarinic receptor subtypes in the pre-BötC are M(2) and M(3), with minor expression of M(1), M(4), and M(5).

Animals↗

PubMed1981

Necrotizing fasciitis.

Fourteen patients with necrotizing fasciitis seen over a 5 year period at a public hospital are reviewed. Middleaged men predominated. The disease followed such diverse initiating causes as self-injection with heroin, boil, ischiorectal abscess, perforated occult colonic cancer and trivial abrasions. In a few cases there was no evidence of an initiating lesion. Necrotizing fasciitis affected the arms, legs, trunk and neck. Bacteriologic analysis showed that the disease is usually caused by gram-negative bacilli and hemolytic streptococci, alone or in combination. Morbidity and mortality rates in the present series were influenced by associated clinical conditions such as old age, diabetes mellitus, carcinoma and gram-negative bacteremia.

Adult↗

PubMedDate not supplied

Comparison of brachial and calf blood pressures in infants.

PURPOSE: To compare brachial and calf blood pressures in infants under one year of age using the same size blood pressure cuff. METHODOLOGY: A convenience sample of 79 infants ages 4-300 days was selected from the General Pediatric, Pediatric Special Care, and Pediatric Intensive Care units in an acute care hospital located in the southern United States. Difference between brachial and calf blood pressures were compared using a paired t-test and defining the range of difference for 95% of the sample. RESULTS: There was no statistically significant difference in brachial and calf blood pressure measurements when assessed by paired t-test. The range of difference for 95% of the sample merits further clinical attention. CONCLUSION: Calf blood pressure measurements can be assessed routinely on infants under one year of age but it is important to consistently compare calf pressures to calf pressures and brachial pressures to brachial pressures.

Age Factors↗