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Biomedical subjects

E E Graham

Publications and source records attributed to E E Graham.

6 recordsLinked to original sources
PubMed1985

Isolation of enamelinlike proteins from blue shark (Prionace glauca) enameloid.

A sequential dissociative extraction scheme was used to extract proteins from developing Blue Shark enameloid. The first extraction solution (4 M guanidine HC1) solubilized the polypeptides, mainly collagenous, not closely associated with the hydroxyapatite. The next extraction solution (4 M guanidine HC1, 0.5 M ethylenediaminetetraacedic acid (EDTA] solubilized the proteins more closely associated with the tooth mineral component. After extraction, the proteins were separated and isolated with gel electrophoresis. Protein molecular weights were determined and selected proteins were isolated for amino acid composition analysis. The two proteins isolated were tested for mammalian enamel protein antigenic determinants by a "Dot" immunobinding assay. The isolated proteins were enamelinlike by extraction criteria and amino acid composition. Further, the two proteins share antigenic determinants with mammalian enamel proteins.

Amino Acids↗
PubMed1984

Protein biosynthesis during spiny dogfish (Squalus acanthias) enameloid formation.

Little is known about protein biosynthesis during enameloid formation. The purpose of this study was to characterize protein biosynthesis during selachian amelogenesis. Experiments were conducted on intact shark tooth organs, surgically removed from the spiny dogfish, Squalus acanthias. These organs were cultured in medium labelled with [35S]-methionine during time periods ranging from 1 to 6 h in pulse/chase experiments. The proteins were extracted at the conclusion of the experiments for analysis by gel electrophoresis and fluorography. In addition, tooth organs were fixed and examined by light microscopic autoradiography. Through this outlined experimental strategy, protein biosynthesis during amelogenesis was examined. The findings suggest the ameloblasts, at early bell stage of tooth development, are biosynthetically more active than the incipient odontoblasts; and they are secreting two polypeptides, 55 and 43 kd, into the developing tooth extracellular matrix.

Ameloblasts↗
PubMed1980

A probable submandibular gland defect in a prehistoric mandible.

The mandible of a prehistoric male Indian, 35 to 40 years old at death, displays a probable bilateral submandibular gland defect. This defect is described, its incidence in prehistoric and modern populations is reviewed, and a possible genetic etiology is proposed.

Adult↗
PubMed1976

Decreasing postpartum sexual abstinence time.

Postpartum sexual abstinence time can be safely shortened for most patients when episiotomy repair is done meticulously with fine PGA suture on small needles. The time preferred by patients for resumption of intercourse seems to be between the second and third postpartum week. We have seen no ill effects from this, and we feel that sexual intercourse at these early dates does not influence the healing of the episiotomy in any way.

Episiotomy↗