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Biomedical subjects

E E Moody

Publications and source records attributed to E E Moody.

13 recordsLinked to original sources
PubMed1979

Is abortive infection by bacteriophage BF23 of Escherichia coli harboring ColIb plasmids due to cell killing by internally liberated colicin Ib?

Infection of Escherichia coli harboring ColIb+ plasmids with bacteriophage BF23+ is abortive and resulted in changes of membrane permeability as measured by efflux of nucleotides and K+. A single pre-early gene product of BF23+ was necessary and sufficient to elicit the abortive response. Appropriate mutations in this pre-early gene allowed a productive infection in ColIb+ cells. Appropriate mutations in the ColIb plasmid also allowed a productive infection with BF23+. A comparison of changes occurring during abortive infection and during killing of sensitive cells by external colicin Ib or Ia, together with certain genetic data, has led to the conclusion that membrane changes accompanying the two phenomena are the result of a common mechanism, namely, the interaction of free colicin with the cytoplasmic membrane.

Bacteriocin Plasmids↗
PubMed1976

Isolation and characterization of mutants of Escherichia coli K12 resistant to the new aminoglycoside antibiotic, amikacin.

Spontaneous mutants of Escherichia coli K12 that are resistant to the new aminoglycoside antibiotic, amikacin, were isolated. These mutants have simultaneously acquired cross-resistance to kanamycin, gentamicin and neomycin but not to streptomycin or spectinomycin. Sensitivity of the mutant strains to the non-aminoglycoside antibiotics, ampicillin, tetracycline and polymyxin, was unaffected. The mutation responsible for amikacin resistance was mapped by PI transduction and found to be tightly linked to strA, distal with respect to spcA and aroE.

Amikacin↗
PubMed1976

Isolation and genetic characterizaion of Escherichia coli K-12 mutations affecting bacteriophage T5 restriction by the ColIb plasmid.

A mutant derivative of Escherichia coli K-12 has been isolated which is permissive for bacteriophage T5 infection even when harboring a wild-type ColIb plasmid. The fully permissive phenotype was the result of two mutations that are located near the rpsL-rpsE region on the E. coli chromosome and are recessive to the wild-type alleles. These mutations had little or no effect on induction of colicin synthesis and did not affect the expression of antibiotic resistance by the resistance plasmids R64drd11 or R1drd19. Cells harboring the mutant alleles grew more slowly than isogenic wild-type derivatives in either minimal or complete media.

Alleles↗
PubMed1975

DNA repair synthesis dependent on the uvrA,B gene products in toluene-treated cells.

Ultraviolet irradiation of toluene-treated Escherichia coli causes an inhibition of replicative DNA synthesis. This is followed by the appearance of nonconservative DNA repair synthesis which does not require either the polymerase or 5' leads to 3' exonucleolytic activities of DNA polymerase I. The repair synthesis may be catalyzed by DNA polymerase III activity but does not require a functional DNA polymerase II. The ultraviolet-induced synthesis requires ATP and is dependent on a functional uvrA and uvrB gene product. However, other uvr gene products are not required for the synthesis. The recB function is also not required.

Conjugation, Genetic↗
PubMed1975

Bacteriophages and endotoxin in licensed live-virus vaccines.

In confirmation of recent reports, coliphages were found in seven of 19 unselected samples of the currently licensed live-virus vaccines. Coliphages and pseudomonas phage were found in 11 and 14, respectively, of the 20 bovine sera commonly used in the cell culture phase of virus vaccine production. The same lots of vaccine and serum were examined by the limulus assay for endotoxin, another product of bacterial contamination. Eighteen of 20 sera had detectable endotoxin-like activity. Our preliminary results suggest that endotoxin activity may serve as a sensitive indicator of residual products of previous bacterial contamination, including bacteriophages.

Animals↗
PubMed1972

Chromosome transfer by autonomous transmissible plasmids: the role of the bacterial recombination (rec) system.

The ability of autonomous transmissible plasmids or sex factors to transfer chromosomal genes to F(-) recipient bacteria has been investigated by using a series of rec(+) and recA(-) donor strains. It is concluded that chromosome transfer by most sex factors is virtually dependent upon the functional integrity of the bacterial recombination system. However, evidence is presented that suggests the existence of plasmid-specified mechanisms of interaction with the chromosome which are independent of the bacterial recombination system.

Anti-Bacterial Agents↗
PubMedDate not supplied

A solid-phase radioimmunoassay for the detection of antibodies to Treponema pallidum.

A solid-phase radioimmunoassay (RIA) procedure was developed for the detection of antibodies to Treponema pallidum. The source of antigen used was T. pallidum (Nichols strain), which was specifically bound to the solid phase by use of rabbit antibody to T. pallidum (Nichols strain). The solid-phase system for this RIA test is the well of a microtiter plate. Preliminary data suggest that the RIA procedure is sensitive and specific and might be a useful addition to the research tools and serologic tests used in the study and diagnosis of syphilis.

Antibodies, Bacterial↗