PubMed Health⌕ Search

Biomedical subjects

E E Woodside

Publications and source records attributed to E E Woodside.

7 recordsLinked to original sources
PubMed1968

Protein-polyelectrolyte interactions. The concanavalin A precipitin reaction with polyelectrolytes and polysaccharide derivatives.

1. Concanavalin A formed precipitates with polyelectrolytes such as fucoidan, RNA, heparin and bacterial lipopolysaccharides. 2. Precipitate formation also occurred between ficoll and concanavalin A. 3. Precipitate formation between concanavalin A and dextran or soluble starch was induced by the incorporation of phosphate groups into the unreactive glucans. 4. Introduction of polar groups, such as acetate, formate and phosphate, into glycogen resulted in enhanced precipitation with concanavalin A, whereas the opposite effect was noted on incorporation of hydrophobic (methyl) centres. 5. Neutral sugars and salt partially inhibited complex-formation between polyelectrolytes and concanavalin A. 6. Concanavalin A-glycogen complexes could be dissociated with 5% (w/v) trichloroacetic acid or 44% phenol-water. 7. Concanavalin A lost its glycogen-complexing ability after phenol treatment. 8. Evidence is presented for the existence of common binding sites on concanavalin A for both neutral polysaccharides and polyelectrolytes. 9. Hydrogen bonding appeared to play a major role in neutral polysaccharide-concanavalin A precipitate formation, whereas both hydrogen bonding and electrostatic forces were implicated in polyelectrolyte-concanavalin A complex-formation.

Acetates↗
PubMed1964

Carbohydrate and lipid content of radiation-resistant and -sensitive strains of Escherichia coli.

Woodside, E. E. (U.S. Army Medical Research Laboratory, Fort Knox, Ky.), and W. Kocholaty. Carbohydrate and lipid content of radiation-resistant and -sensitive strains of Escherichia coli. J. Bacteriol. 87:1140-1146. 1964.-Total lipid contents of acetate minimal medium cultures of Escherichia coli, strains B, B/r, and B(s), were not significantly different when identical pretreatment and extraction procedures were compared. Wide variations in intracellular hexose and pentose derivatives of E. coli B, B/r, and B(s) were induced by changes in carbon and nitrogen sources and by changes in the growth phases. The three strains produced more intracellular carbohydrate when grown in nutrient broth-glucose medium than when grown in unsupplemented nutrient broth. Acetate minimal medium cultures of the radiation-sensitive mutant, E. coli B(s), contained the least, and the radiation-resistant mutant, E. coli B/r the largest, amounts of intracellular hexoses. Environmental conditions which increased the radiation resistance of E. coli B/r were similar to the environmental conditions which favored increased intracellular hexose accumulation. After X ray of E. coli B/r, considerable amounts of hexoses and pentoses were released into the growth medium. Alterations in hexose distribution patterns of X-rayed E. coli B/r preceded alterations in pentose distribution patterns. Prolonged postirradiation incubation resulted in a net synthesis of extracellular hexose, with concomitant loss of intracellular hexose accumulation.

Acetates↗