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Biomedical subjects

E F Kaleta

Publications and source records attributed to E F Kaleta.

At least 19 recordsLinked to original sources
PubMed1996

Local and systemic specific antibody response of different chicken lines after ocular vaccination against infectious bronchitis.

The specific lacrimal fluid IgA levels and the specific serum IgG levels of broiler chicks (meat type hybrids (MT)), brown-egg layer chicks (heavy layer (HL)), and white leghorn chicks (light layer (LL)) were compared after infectious bronchitis virus (IBV) ocular vaccination at 1 day of age. All birds were maintained as a mixed population throughout the experiment of 45 days. The class specific antibody levels were determined at regular intervals by enzyme-linked immunosorbent assays. All birds responded to the vaccination stimulus as shown by a significant increase of antibody levels in both serum and lacrimal fluid. When comparing the IgG response of the chicken lines tested, LL chicks showed higher serum IBV-IgG values at the time of maximal response at days 5 and 9 post-vaccination (pv). This bird group also showed a more homogeneous (lowest coefficient of variation of values) serum IgG response. On day 13 pv and until the last serum sampling day (day 41 pv) all three chicken types showed statistically identical serum IBV-IgG levels. The local IgA response detected in lacrimal fluids showed differences between the chicks at the time of maximal levels (days 5 to 14 pv), the response of LL chicks being the highest. LL chicks maintained higher specific IgA levels than MT and HL chicks almost throughout the experimental period. According to the coefficient of variation of the absorbance values (36%), the IgA response shown by LL chicks was the most homogeneous.

Animals↗
PubMed1994

Recent influenza A (H1N1) infections of pigs and turkeys in northern Europe.

The most recent introduction of an avian influenza A virus without reassortment into mammals occurred in 1979 when H1N1 strains could be isolated from diseased pigs in northern Europe. This newly introduced avian virus formed a stable lineage in pigs and, in the meantime, spread all over Europe. In 1991 highly pathogenic H1N1 strains closely related to a contemporary swine virus were isolated from turkeys of a breeding farm near Bremen, Germany. Outbreaks in several farms in Germany, France, and the Netherlands indicate that the "avian-like" swine viruses can easily be reintroduced into an avian population causing severe economical losses.

Alleles↗
PubMed1994

[A herpesvirus-caused enzootic--Pacheco's parrot disease--in a psittacine collection].

After the official confirmation of psittacosis in a collection of psittacine birds a total of 40 of them died during treatment with tetracycline. 36 of them underwent post mortem examination. From 33 birds the causative herpesvirus of Pacheco's parrot disease (PPD) was isolated and/or a non-purulent hepatitis diagnosed, the latter a characteristic for PPD. The cause of the outbreak was assumed to be a latent herpesvirus infection of individual birds which was activated by various stress factors during the psittacosis treatment. The macroscopic and histologic lesions, the results of virological investigations and the in vitro effect of acyclovir on the multiplication of the isolated herpes virus are described.

Animals↗
PubMed1994

[Detection, significance and control of paramyxoviruses in birds].

Infection, disease and mortality due to various paramyxoviruses currently occur fairly frequently in both domestic fowl and in captive and free-living birds. These viruses are therefore of economic and ecologic significance, and may also cause zoonoses. The current legal status of the detection and control of paramyxovirus-associated diseases or epidemics are described. Prophylactic vaccination against Newcastle disease using live and inactivated vaccines, as required by the fowl disease regulations, remains of great importance in maintaining health and productivity of the fowl. Correct immunisation also includes checking the effectiveness using serological methods.

Animals↗
PubMed1994

[Immunoprophylaxis against Newcastle disease in white storks: vaccine selection and preliminary experiences].

Criteria for the selection of vaccines against Newcastle disease are presented. Questions related to safety and potency are considered. Intramuscular vaccination of 23 young storks with an inactivated vaccine is described. All storks tolerated the vaccination without recognizable undesired side-effects. The young storks reacted with the production of serum antibodies three weeks post vaccination. No data is yet available on the duration of the persistence of humoral antibodies. The vaccinated mature storks were free of detectable antibodies at the day of vaccination and five months later.

Age Factors↗
PubMed1992

[Comparative pathogenicity tests of eight pigeon paramyxovirus 1 variant isolates in pigeons, turkeys and chickens].

Eight pigeon paramyxovirus-1 isolates which were isolated from diseased pigeons were comparatively tested for their pathogenicity in chickens, turkeys and racing pigeons. Intramuscular inoculation of all of the eight viruses resulted in all pigeons in clinical signs like polyuria, lameness of wings and in parts also in torticollis. Also, intravenously inoculated chickens developed distinct signs such as apathy, liquid droppings and in part also torticollis. Six of the eight PMV-1 isolates induced in turkeys similar signs as in chickens; inoculation of two isolates yielded no signs in turkeys. Legal sanitary consequences of the disease due to pigeon PMV-1 infection in chickens and in turkeys should be identical to that of velogenic Newcastle disease.

Animals↗
PubMed1992

Isolation and identification of psittacid herpesvirus 1 from imported psittacines in South Africa.

Acute deaths occurred in 47 out of a total of 131 imported psittacine birds whilst in quarantine. Few and non-specific clinical signs were seen during the course of the disease outbreak, but gross pathology revealed severe hepatomegaly and splenomegaly. A herpes virus was isolated from liver and spleen material taken from 2 birds, an Amazon (Amazona aestiva aestiva) and a Yellow-collared macaw (Ara auricollis). Identification procedures included virus neutralisation tests carried out in chicken embryo fibroblast cultures. Neutralisation of the virus was obtained by antisera to Psittacid herpesvirus type 1 (HV1) but not against HV2 or HV3.

Animals↗
PubMed1991

[Isolation of a paramyxovirus-3 from turkeys with respiratory tract disease in Germany].

In fattening turkeys 2.5 weeks of age a respiratory disease associated with coughing, nasal discharge and swelling of the infraorbital sinus was seen. Pathological findings in diseased turkeys were sinusitis, tracheitis, pneumonia and aerosacculitis. Virological investigations of trachea, kidney and intestine in SPF-chicken embryos resulted in the isolation of a virus, that could be identified as a paramyxovirus type 3 due to chemical-physical, biological, morphological and immunological properties. The pathogenicity of the isolate 324/86 to turkeys was shown in a test with three weeks old turkey poults. This is the first isolation and identification of a paramyxovirus-3 of turkeys in Germany.

Animals↗
PubMed1989

Crane hepatitis herpesviruses.

Comparative studies were performed on crane herpesviruses obtained from two different enzootics in Austria and France. The examined viruses appear to be identical in their physico-chemical properties, morphology and biological reactions in ovo. The crane viruses are tentatively classified as beta-herpesviruses. Crane herpesvirus antisera produced in rabbits reacted in cross neutralization tests with each other and with a herpesvirus obtained from a bobwhite quail. No reactivity was observed with other avian herpesviruses and with human herpes simplex virus type 1 and 2.

Animals↗
PubMed1989

Polyomavirus infection in budgerigars (Melopsittacus undulatus): clinical and aetiological studies.

In order to get insight into the aetiology of French Moult (FM) and Budgerigar Fledgling Disease (BFD), and to determine relationships between the two diseases, 298 budgerigars from 49 different breeding flocks were examined. From all birds with BFD and from several birds with FM, viruses were isolated which produced characteristic cytopathic changes in chicken embryo fibroblasts. They were insensitive to chloroform treatment, and their replication was inhibited in the presence of 5-iododeoxyuridine. One of these isolates, from a bird exhibiting clinical signs of BFD, was determined by biochemical and biophysical methods to be a polyomavirus (BFDV). Nestling budgerigars 3 to 10 days of age, were inoculated with this BFDV isolate. They developed an acute systemic disease with high mortality rates, similar to naturally occurring infections. In this regard, BFDV differs markedly from all the other polyomaviruses known until now which only cause clinically asymptomatic, persistent infections in their natural hosts. Most of the birds examined with clinical signs of BFD or FM exhibited low titers of antibodies neutralizing the BFDV isolate, whereas in clinically healthy birds from flocks that never had any problems with FM or BFD, no antibodies against BFDV could be detected. On account of the results of our experiments described here we suspect that FM might be a milder, more protracted form of a BFDV infection.

Animals↗
PubMed1989

[Therapy trials with enrofloxacin (Baytril) in a laying hen flock with Salmonella pullorum infections].

The course of a disease due to Salmonella pullorum in laying chickens is described. The experimental application of enrofloxacin (Baytril) at a dosage of 100 mg/l drinking water for 10 days resulted in a stop of the mortality rate, increment of egg production and reduction of Salmonella excretion. A recurrence of the disease was observed in the fifth week following the end of treatment with high mortality and decreasing egg laying. The treatment lead to a short-dated success but a permanent elimination of the germ from the flock was not achieved.

4-Quinolones↗
PubMed1988

Isolation and characterization of a hepatitis B virus endemic in herons.

A new hepadnavirus (designated heron hepatitis B virus [HHBV]) has been isolated; this virus is endemic in grey herons (Ardea cinerea) in Germany and closely related to duck hepatitis B virus (DHBV) by morphology of viral particles and size of the genome and of the major viral envelope and core proteins. Despite its striking similarities to DHBV, HHBV cannot be transmitted to ducks by infection or by transfection with cloned viral DNA. After the viral genome was cloned and sequenced, a comparative sequence analysis revealed an identical genome organization of HHBV and DHBV (pre-C/C-, pre-S/S-, and pol-ORFs). An open reading frame, designated X in mammalian hepadnaviruses, is not present in DHBV. DHBV and HHBV differ by 21.6% base exchanges, and thus they are less closely related than the two known rodent hepatitis B viruses (16.4%). The nucleocapsid protein and the 17-kilodalton envelope protein sequences of DHBV and HHBV are well conserved. In contrast, the pre-S part of the 34-kilodalton envelope protein which is believed to mediate virus attachment to the cell is highly divergent (less than 50% homology). The availability of two closely related avian hepadnaviruses will now allow us to test recombinant viruses in vivo and in vitro for host specificity-determining sequences.

Amino Acid Sequence↗
PubMed1987

Newcastle disease vaccine (La Sota) strain specific monoclonal antibody. Brief report.

Newcastle disease virus vaccine strain (La Sota) specific monoclonal antibody (La-1) was produced by immunizing mice with isolated glycoproteins of strain La Sota. This antibody was recognized only in the ELISA test in which it bound exclusively to La Sota strain out of a range of over 300 lentogenic, mesogenic and velogenic strains examined.

Animals↗