Biomedical subjects

E F Morato

Publications and source records attributed to E F Morato.

5 recordsLinked to original sources

PubMed1997

Conditioned immunosuppression of lipopolysaccharide-induced antibody response of orally immunized mice.

BALB/C mice previously conditioned in a paired regimen in which cycophosphamide was given in association with a novel taste in the drinking water (saccharin, SAC) subsequently received LPS (1mg/mouse) by oral gavage. Subgroups of mice were reexposed to SAC or water respectively, and anti-LPS antibody production measured 6 days later using an ELISA/ELISPOT assay. Our data show that the immune response to this T-independent antigen, administered orally, is amenable to conditioned immunosuppression in this model system.

Administration, Oral↗

PubMed1996

Conditioned immunosuppression in orally immunized mice.

Mice were given oral immunization after pretreatment with a regimen (cyclophosphamide and a novel taste in the drinking water, chocolate milk (CHM), which leads to suppression of the antibody response to intravenously administered antigens given concurrently with CHM. Following this treatment mice were reexposed to CHM and IgM and IgA antibody forming cells (AFC) were measured in spleen and Peyer's patch cells. Conditioned immunosuppression of AFC production was most marked (> fivefold) for IgA-AFC in Peyer's patch, with effects of lesser magnitude for IgM-AFC in Peyer's patch (twofold) and both IgM- and IgA-AFC in spleen. Analysis of cytokine production from stimulated Peyer's patch and splenic T cells in vitro showed significant decreased production of both IL-2 and IL-4, with the latter being the predominant cytokine produced in Peyer's patch cells of control animals.

Administration, Oral↗

PubMed1993

Differential effects of ethanol in mouse lines genetically selected for high and low antibody production.

There is considerable evidence of a relationship between the central nervous system and the immune system, and that several drugs with primary action at the central nervous system can affect different immune responses. In an attempt to obtain a better understanding of the interaction between ethanol's effects and immune functions, we used two mouse lines selected according to their high (HGP) or low (LGP) antibody production. These animals were injected with ethanol (20% w/v, 3.6 g/kg, ip); the latency for the loss of the righting reflex (LORR) and the time for the recovery of the righting reflex (RORR) were measured. Body temperature of both lines was also recorded before the injections and at RORR. Blood samples were taken at RORR to determine blood ethanol concentration. The results showed that the HGP mice presented shorter latency for sleep, longer sleeping time, and lower blood ethanol levels than the LGP mice. These data suggest that the HGP line is more sensitive to the effects of ethanol, and provide an additional approach for studying interactions between the immune and the central nervous systems.

Animals↗

PubMed1992

Effects of indomethacin, aspirin, and acetaminophen on ethanol diuresis in rats.

A number of studies have indicated that prostaglandin synthetase inhibitors (PGSI) can antagonize several effects of ethanol. This study examines the influence of intraperitoneal pretreatment with the PGSI indomethacin (5.0 or 10.0 mg/kg), aspirin (15.0 or 30.0 mg/kg) and acetaminophen (15.0 or 30.0 mg/kg) on the diuretic effect produced by orally administered ethanol (4.0 g/kg) in female rats. Pretreatments with indomethacin and aspirin led to an antagonism of ethanol's diuretic effect. Nevertheless, acetaminophen failed to antagonize this effect. These results are in agreement with data from the literature on PGSI versus ethanol and suggest possible involvement of prostaglandins in the diuretic effect of ethanol.

Acetaminophen↗

PubMed1989

Chemical and pharmacological studies on Talauma ovata St. Hil. (Magnoliaceae).

Antidiabetic activity of crude extract of leaves of Talauma ovata St. Hil. (Magnoliaceae) was analysed as part of a general pharmacological screening of this plant. Chemical analysis demonstrated the presence of phytosteroids, saponins, alkaloids and tannins in the crude extract. Pharmacological studies failed to demonstrate hypoglycemic effect of this plant in normoglycemic, hyperglycemic or alloxan-diabetic rats. The low LD50 obtained for this plant strongly suggests that its consumption by the population may be hazardous.

Animals↗