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Biomedical subjects

E Farrell

Publications and source records attributed to E Farrell.

At least 37 records · Page 2Linked to original sources
PubMed1987

The bases of the tRNA anticodon loop are independent by genetic criteria.

We employed two methods to study the translational role of interactions between anticodon loop nucleotides. Starting with a set of previously constructed weakly-suppressing anticodon loop mutants of Su7, we searched for second-site revertants that increase amber suppressor efficiency. Though hundreds of revertants were characterized, no second-site revertants were found in the anticodon loop. Second site reversion was detected in the D-stem, thereby demonstrating the efficacy of the search method. As a second method for detecting interactions, we used site-directed mutagenesis to construct multiple mutations in the anticodon loop. These multiple mutants are very weak suppressors and have translational activities that are equal to or lower than that predicted for the independent action of single mutations. We conclude that although the anticodon loop sequence of Su7 has an optimal structure for the translation of amber codons, we find no evidence that interactions between loop bases can enhance translational efficiency.

Anticodon↗
PubMed1985

Physical, chemical, and physiological characteristics of isolates of pulmonary surfactant from adult rabbits.

Physical and chemical characteristics of two types of preparations of surface active material from adult rabbits were determined. A procedure using multiple centrifugations produced a surface active material (type A) which had 6.6% by weight protein and a phosphorus/protein ratio of 13.1 nmol P/microgram protein. A simpler protocol involving two centrifugations yielded a surface active material (type B) with more protein (10.8%) and a lower phosphorus/protein ratio (8.4 nmol/micrograms). Lipid compositions of both types were similar with phosphatidylcholine being the major phospholipid (80%) and palmitate the major fatty acid in the total lipid (65-71%) and in phosphatidylcholine (80%). Both types exhibited broad thermotropic phase transitions encompassing 37 degrees C. Measurements of aqueous dispersions of surface active material on the surface of a Langmuir-Whilhelmy balance or in a pulsating bubble apparatus indicated that there was variability both between types and between batches of the same type in the capacity to reach low surface tension on the surface balance and in the rates at which low surface tension was achieved on the bubble apparatus. Type A preparations were somewhat more reliable in meeting these ends than were type B. Both types of isolates were effective in normalizing pressure-volume characteristics when instilled into the lungs of immature rabbit fetuses.

Animals↗
PubMed1985

The effect of anaesthetics on the uptake of brain-imaging agents in rats.

Anaesthetics are shown to affect the brain uptake of two pH-shift brain imaging agents, di-B-(morpholinoethyl)-selenide and N,N,N'-trimethyl-N'-(2-hydroxy-3-methyl-5-iodobenzyl)-1,3-propanediamine significantly. This is probably due to changing regional cerebral blood flow. The present study reaffirms previous observations that small disturbances such as stress and type of anaesthesia will significantly alter the regional cerebral blood flow and, as a consequence, brain uptake in rats. This is an important factor to consider when measuring or comparing brain uptake data.

Anesthetics↗
PubMed1981

A controlled trial of fetal heart rate monitoring in a low-risk obstetric population.

A controlled trial of fetal heart rate (FHR) monitoring in a low-risk obstetric population was carried out in 989 patients at the Mercy Maternity Hospital and Queen Victoria Medical Centre. The use of monitoring failed to demonstrate any improvement in perinatal outcome. Monitoring was associated with an increased intervention rate and a small increase in the number of babies who remained in the isolette beyond 3 days. This study does not support the view that FHR monitoring should be used in all labors. Further study in a larger sample is recommended.

Adult↗
PubMed1974

Survival of R+ Escherichia coli in sea water.

The survival of Escherichia coli strains in sea water appears not to be affected by the possession of an R(+) factor. Sea water induces no detectable curing of R(+)E. coli.

Anti-Bacterial Agents↗