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E Fifkova

Publications and source records attributed to E Fifkova.

At least 19 recordsLinked to original sources
PubMed1998

Focal stimulation of the thalamic reticular nucleus induces focal gamma waves in cortex.

Electrical stimulation of the thalamic reticular nucleus (TRN; 0.5-s trains of 500-Hz 0.5-ms pulses at 5-10 microA) evokes focal oscillations of cortical electrical potentials in the gamma frequency band ( approximately 35-55 Hz). These evoked oscillations are specific to either the somatosensory or auditory cortex and to subregions of the cortical receptotopic map, depending on what part of the TRN is stimulated. Focal stimulation of the internal capsule, however, evokes focal slow potentials, without gamma activity. Our results suggest that the TRN's role extends beyond that of general cortical arousal to include specific modality and submodality activation of the forebrain.

Acoustic Stimulation↗
PubMed1992

Aging and the neurocytoskeleton.

It has been often demonstrated that during senescence some neurons undergo atrophic changes while others add new processes and terminals. Because microtubules form a substantial component of the dendritic and axonal cytoskeleton, we have studied the amount of tubulin and acetylated alpha-tubulin in three young (6 months) and three old (24 months) rats (Fischer 344). We have used sodium dodecyl sulfate (SDS) extracts of brain homogenates and Triton solubilized fractionated brain homogenates. With the first method we did not detect any age-related differences in total brain protein, total tubulin, or in relative amounts of acetylated alpha-tubulin. With the second method, we have observed a small systematic increase in relative amount of acetylated alpha-tubulin in the Ca2+/cold insoluble fraction. These results are similar to those reported in the literature, and they indicate a possible alteration in the cytoskeletal dynamics.

Acetylation↗
PubMed1990

Nuclear pore complex frequency in CA1 pyramidal cells of the aging rat.

The frequency and the diameter of nuclear pore complexes, and the nuclear perimeter, were studied in CA1 pyramidal cells of the hippocampi from 3-, 9-, 24-, and 30-month-old rats (Fischer 344). No changes with age in any of these parameters were observed. This finding is discussed in terms of varied responses of different brain areas to the effects of aging.

Aging↗
PubMed1989

Distribution of MAP2 in dendritic spines and its colocalization with actin. An immunogold electron-microscope study.

The distribution of MAP2 and actin in dendritic spines of the visual and cerebellar cortices, dentate fascia, and hippocampus was determined by using immunogold electron microscopy. By this approach, we have confirmed the presence of MAP2 in dendritic spines and identified substructures within the spine compartment showing MAP2 immunoreactivity. MAP2 immunolabeling was mainly associated associated with filaments which reacted with a monoclonal anti-actin antibody. Also, by immunogold double-labeling we colocalized MAP2 with actin on the endomembranes of the spine apparatus, smooth endoplasmic reticulum, and in the postsynaptic density. Labeling was nearly absent in axons and axonal terminals. These results indicate that MAP2 is an actin-associated protein in dendritic spines. Thus, MAP2 may organize actin filaments in the spine and endow the actin network of the spine with dynamic properties that are necessary for synaptic plasticity.

Actins↗
PubMed1979

A Golgi study of the early postnatal development of the visual cortex of the hooded rat.

Although neuroanatomical plasticity has been demonstrated in the rat visual cortex, no systematic data on the dendritic development of the area are available. In the present study, the visual cortex of hooded rats at 1, 3, 5, 7, 10 and 15 postnatal days of age (P1-P15) was impregnated with the rapid Golgi method. The cortex was divided into the superficial layers, II-IV, and the middle layer V. At P1, pyramidal neurons had apical shafts and the beginning of the apical terminal arch. Analysis of both basilar and oblique dendritic number showed that pyramidal neurons of the middle layer developed more quickly than those in the superficial layers. The number of lower order basilar dendritic branches reached asymptote over the examined time period, whereas the higher order branches were still increasing in number but at a decelerating rate by P15. Dendrites at all ages exhibited varicosities which were especially prominent on the thin dendritic branches of the earlier ages. Some thin, filamentous processes, termed protospines, were found on dendrites and cell bodies at P1 to P5. They seemed to decrease by P7, when a few mature spines appeared. Spines increased in number on days P10 and P15. A comparison of the data from this study with quantified Golgi studies in adult rats indicates that by P10 and P15 the number of basilar branches is in the range seen in the adult.

Age Factors↗
PubMed1979

An electron microscope study of the early postnatal development of the visual cortex of the hooded rat.

Synaptic plasticity in response to environmental events has been clearly demonstrated in the visual cortex of the rat, but no detailed data concerning the course of early synaptogenesis in this area are available. In this study, synaptogenesis in the visual cortex of hooded rats at 1, 3, 5, 7 and 10 postnatal days of age (P1-P10) was examined with electron microscopy. The cortex was divided into the molecular layer, the superficial layers (II-IV) and deep layers (V-VI). In the visual cortex at P1, very few synapses are present in the molecular and deep layers and virtually none in the yet undifferentiated layers II-IV that compose the cortical plate at this age. The synapses that are present are axodendritic and often symmetrical with little membrane thickening and few vesicles. Axosomatic synapses were seen as early as P3 but very rarely. There are marked increases in axodendritic synaptic density and maturity with increasing age. By P7 and P10, many synapses appear mature in form and the majority can be classified a symmetrical. Axospinal synapses first appeared at P7 and were more frequent by P10. However, this classification was somewhat uncertain since no spinal apparatus was detected. Rate of synaptogenesis appeared to increase over the ages studied and showed no signs of leveling off except in the deep layers. Synaptic length was extremely variable and did not change systematically with age.

Age Factors↗
PubMed1975

Rapid freezing of deep cerebral structures for electron microscopy.

A method is described for the investigation of deep cerebral structures by freeze-substitution. The head of a mouse is sectioned in a guillotine-like apparatus. The exposed surface is subjected to freeze-substitution. Electron micrographs prepared from this material exhibit under a layer of sheared tissue, a layer of tissue comparable with that observed previously in micrographs of the cerebral and cerebellar cortices subjected to freeze-substitution of their natural surfaces. By varying the plane in which the head is sectioned any structure in the brain can be made accessible to freeze-substitution.

Animals↗
PubMedDate not supplied

Effect of age on blood vessels and neurovascular appositions in the rat dentate fascia.

Rats aged 3, 9, 24 and 30 months were used in this study. We show increased basal lamina thickening and increased mitochondrial presence in walls of capillaries and not in walls of large vessel populations with age. This suggests that age selectively affects capillary structure. Ultrastructural differences between capillaries and two types of large vessels are reported and discussed in terms of their probable functional significance. In particular it was noted that there are more axon terminals, axons and dendrites adjacent to capillaries than to large vessels and that this was unaffected by increasing age. It is not clear whether the proximity of neuronal processes to a vessel wall serves a function, however, the larger number adjacent to capillaries than to large vessels indicates a more significant role for them in capillary rather than in large vessel function. Since increasing age did not alter the number of neuronal processes adjacent to vessels, age-related compromises in vessel function may be unrelated to neuronal regulation. The age-related changes are discussed as possible vascular markers for the aging brain.

Aging↗