Biomedical subjects

E Fontes

Publications and source records attributed to E Fontes.

16 recordsLinked to original sources

PubMed2001

Mathematical modelling of physicochemical reactions and transport processes occurring around a platinum cathode during the electrochemical treatment of tumours.

The electrochemical treatment (EChT) of tumours is an anti-tumour therapy in which a continuous direct current is applied to electrodes, placed in or near a tumour. Promising results have been reported from clinical trials in China, where more than 10,000 patients have been treated with EChT during the past 10 years. Before clinical trials can be conducted outside of China, a reliable dose-planning strategy has to be developed. One approach in achieving this is the use of physicochemical simulation models. A simplified mathematical model of the physicochemical processes, occurring around a spherical platinum cathode during EChT, is developed and visualized in three steps in this paper. In the final step, tissue is treated as an aqueous solution of sodium chloride, containing a bicarbonate buffer system and organic constituents susceptible to reactions with hydroxyl ions. This model is shown to give a good description of the pH profile obtained around the cathode after EChT. The simulation results reveal a strong correlation between the pH profiles and size of experimentally measured lesions, thus indicating that it is the spreading of hydroxyl ions that determines the extent of tissue destruction around the cathode. In addition, the simulations indicate that the model could be of use in predicting the size of a lesion produced by EChT.

Animals↗

PubMed2000

Clarification of the ripple phase of lecithin bilayers using fully hydrated, aligned samples.

Aligned samples of lipid bilayers have been fully hydrated from water vapor in a different type of x-ray chamber. Our use of aligned samples resolves issues concerning the ripple phase that were ambiguous from previous powder studies. In particular, our x-ray diffraction data conclusively demonstrate that, on cooling from the L alpha to the P beta' phase, both chiral and racemic samples of dipalmitoyl phosphatidylcholine (DPPC) exhibit phase coexistence of long and short ripples with a ripple wavelength ratio lambda L/lambda S approximately 1.8. Moreover, the long ripple always forms an orthorhombic unit cell (gamma L = 90 degrees), strongly supporting the possibility that these ripples are symmetric. In contrast, gamma S for short ripples was consistently different from 90 degrees, implying asymmetric ripples. We continue to find no evidence that chirality affects the structure of rippled bilayers. The relative thermodynamic stability of the two types of ripples was investigated and a qualitative free energy diagram is given in which the long ripple phase is metastable. Finally, we suggest a kinetic mechanism, involving loss of water, that promotes formation of the metastable long ripple phase for special thermal protocols.

1,2-Dipalmitoylphosphatidylcholine↗

PubMed1998

Protein crystallography using a multilayer monochromator.

A multilayer monochromator was installed on a bending-magnet beamline at the Cornell High Energy Synchrotron Source (CHESS) and was used to provide an unfocused pseudo-monochromatic X-ray beam for protein crystallography experiments. Datasets were collected from lysozyme at room temperature and human methylthioadenosine phosphorylase at 100 K. The wide energy bandpass of the multilayer allowed short exposure times, typically only a few times longer than on a focused multipole wiggler beamline. The diffraction images were processed using unmodified monochromatic data-processing software to yield datasets of good quality. These first measurements demonstrate that multilayer monochromators can be readily applied to the rapid structure determination of many typical-sized macromolecules.

Journal Article↗

PubMed1994