Disseminated lymphangiosarcoma in a dog.
This case is unusual due to its extended clinical course. Clinical, histopathological, and immunohistochemical findings are presented.
Animals↗
Biomedical subjects
E G Clark
Publications and source records attributed to E G Clark.
Type C botulism in cattle in association with a botulism die-off in waterfowl in Saskatchewan.
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Animals↗
Chromosomal aneuploidy associated with spontaneous abortions and neonatal losses in cattle.
Pericardial sac samples from 77 bovine aborted fetuses and stillborn calves were submitted for tissue culture; cells from 55 of these samples were grown successfully in culture. Six of the 55 karyotyped fetuses (10.7%) had an abnormal chromosome complement, in 3 of which (5.5%) the abnormality was probably the cause of death. This level of abnormality is relatively high when one considers that most fetuses were >8 months gestational age. Approximately 5-7% of human stillbirths and 50% of first-trimester aborted fetuses have chromosome anomalies. If a similar situation exists in cattle, as suggested by these data, chromosome abnormalities may be a major cause of early fetal loss in cattle. Most chromosomally abnormal fetuses had multiple malformations, which suggests that the diagnostic use of chromosome analysis is most cost effective for malformed fetuses and newborns. Twins were present in a higher proportion of these fetuses than expected based on their incidence among liveborn cattle.
Abortion, Veterinary↗
Spirochete-associated bovine digital dermatitis.
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Animals↗
The relationship between the presence of Helicobacter pylori, Clostridium perfringens type A, Campylobacter spp, or fungi and fatal abomasal ulcers in unweaned beef calves.
A case-control study involving 30 unweaned beef calves was conducted to determine whether specific species of bacteria or fungi were associated with fatal abomasal ulcer formation. Special microbiological and histological techniques were used to detect Clostridium perfringens type A, Helicobacter pylori, or Campylobacter spp. It has been speculated that these bacteria are potential ulcerogenic agents of unweaned beef calves. Calves were recruited for the study at necropsy, with those dying of either a perforating or a hemorrhagic ulcer representing the cases, and calves of a similar age dying of a disease unrelated to the abomasum representing the controls. Helicobacter pylori was not visualized in or cultured from any of the abomasal tissue samples. Clostridium perfringens type A was isolated from 78.6% of the cases and 75% of the controls. These isolates were further dichotomized into "heavy" and "light" growth; no significant association was found between ulcers and the amount of growth. A light growth of Campylobacter spp. was recovered from 3 cases and 3 controls. There was no compelling evidence to suggest that Clostridium perfringens type A, Helicobacter pylori, or Campylobacter spp. were involved in ulcer formation.
Abomasum↗
Screening of depigmenting compounds for the development of an alternate method of branding beef cattle.
The impetus to find alternative methods to stressful routine management procedures has increased in recent years in the hope of improving animal welfare. The potential of using depigmenting compounds to create a permanent visible mark was evaluated in two beef heifers. Eight compounds (hydroquinone [H], 4-ethoxyphenol [4-EP], 4-methylcatechol [4-MC], 4-tert-butylcatechol [4-t-BC], 4-methoxyphenol [4-MP], monobenzone [M], hydroquinone bis (2-hydroxyethyl) ether (HHEE), and catechol [C]) were injected intradermally at concentrations of 10 and 20%. These compounds were screened in four different carriers (ethanol, glycerol, propylene glycol, and liposome cream) to test their effectiveness in producing depigmented hair. Transdermal patches containing 5, 7, and 10% liposome preparations of 4-MC and 4-MP produced depigmentation, but this depigmentation was not as visible as when the same preparations were injected. Histological examination of biopsies and observations of the injected sites were compared to sites that were only injected with the carrier solution. Compounds H, 4-EP, 4-MC, 4-t-BC, 4-MP, and M produced visible depigmentation lasting up to 3 mo. The duration of the effect seemed dependent on the hair cycle length and stage of cycle at time of application. None of the depigmenting compounds tested produced a permanent depigmenting effect.
Administration, Cutaneous↗
Open vs closed-system endotracheal suctioning: a cost comparison.
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Cost Savings↗
Lack of effect of trimethoprim and sulfadiazine in combination in mid- to late gestation on thyroid function in neonatal dogs.
Four pregnant bitches were randomly selected and were given 25 mg sulfadiazine kg-1 and 5 mg trimethoprim kg-1 orally once daily, starting on day 29 of dioestrus until parturition. Five untreated pregnant bitches were used as controls. At the end of the treatment period (parturition = week 0), one to three pups of each litter, treatment and control group, were randomly selected and blood was obtained by cardiac puncture for serum thyroxine (T4) and triiodothyronine (T3) concentrations. These pups were then killed and the thyroids were harvested and examined histologically. This protocol was repeated three times at weekly intervals. There were no significant differences in mean T4 and T3 concentrations between treated and control groups at any time during the study. Mean concentration of serum T4 and T3 did increase significantly over the 3 weeks of study. Histologically, no significant differences in thyroid structure were observed between treated and control groups. The numbers of stillborn or weak pups did not increase in the treatment group. No differences were observed in the length of gestation between the treated and control groups. Administration of sulfadiazine and trimethoprim does not affect the thyroid gland in the neonate.
Animals↗
Immunohistochemical detection of swine influenza A virus in formalin-fixed and paraffin-embedded tissues.
An avidin-biotin complex (ABC) immunohistochemical method utilizing a commercially-available polyclonal antiserum to human influenza A virus was used to detect antigens of influenza A virus in formalin-fixed, paraffin-embedded tissues of swine. Influenza A antigens were immunohistochemically detected in 28/30 cases in which influenza A virus was demonstrated by virus isolation and in 5/22 cases suspected to be influenza A-infected by clinical and histological criteria, but from which the virus was not isolated. Viral antigen was not demonstrated in 30/30 cases not suspected clinically or histologically to be associated with influenza infection. This method is a convenient, sensitive, and specific means of influenza A virus detection and is applicable to both routine diagnosis of influenza A virus infection and to retrospective and prospective studies of the occurrence and the pathogenesis of this virus in pigs.
Animals↗
Monoclonal antibody-based immunohistochemical detection of bovine viral diarrhea virus in formalin-fixed, paraffin-embedded tissues.
Thirty-two monoclonal antibodies directed against epitopes on bovine viral diarrhea virus proteins and glycoproteins were tested for immunohistochemical reactivity with bovine viral diarrhea virus in formalin-fixed and paraffin-embedded tissues from 45 cases of bovine viral diarrhea virus-associated mucosal disease. Only one antibody, designated 15C5, which reacts with the 48-kD glycoprotein of bovine viral diarrhea virus, detected an epitope preserved in these specimens. Monoclonal antibody 15C5 and a polyclonal antibody to bovine viral diarrhea virus successfully detected bovine viral diarrhea viral antigens in 44/45 cases of mucosal disease and did not react with formalin-fixed tissues from 30 uninfected cattle. Monoclonal antibody-based immunohistochemical detection of bovine viral diarrhea virus is routinely fixed tissue specimens has advantages over other currently available techniques in terms of the convenience of specimen submission, the relative ease of method standardization, and the rapidity of the test, and by enabling identification of the virus in association with specific tissues, cell types, and histologic lesions.
Animals↗
Monoclonal and polyclonal antibodies for immunohistochemical detection of bovine parainfluenza type 3 virus in frozen and formalin-fixed paraffin-embedded tissues.
Accurate identification of bovine parainfluenza type 3 virus in bovine respiratory disease requires dependable, sensitive, and specific techniques for detection in affected animals. Immunohistochemical testing can be a rapid and reliable means of demonstration of virus in tissues from suspect cases; however, this procedure is dependent upon the quality of the antisera directed against the viral antigens. The production of rabbit polyclonal and murine monoclonal antibodies directed against bovine parainfluenza type 3 virus and techniques for their use in fresh-frozen and formalin-fixed paraffin-embedded tissues in immunofluorescence and immunoperoxidase-based immunohistochemical tests are described.
Animals↗
Distinctive blunt force injuries caused by a crescent wrench.
Prompt recognition of patterned injuries by the forensic pathologist can greatly facilitate the investigation and resolution of injury and death cases. Careful observation and description of injuries may provide identification of an assault or murder weapon and contribute to the reconstruction of circumstances of injury. The two cases herein described manifest unusual and distinctive injuries resulting from multiple impacts by adjustable crescent wrenches. Detailed photographs and descriptions of these injuries are provided.
Adult↗
Experimental reproduction of respiratory tract disease with bovine respiratory syncytial virus.
An experiment was conducted to reproduce respiratory tract disease with bovine respiratory syncytial virus (BRSV) in one-month-old, colostrum-fed calves. The hypothesized role of viral hypersensitivity and persistent infection in the pathogenesis of BRSV pneumonia was also investigated. For BRSV inoculation a field isolate of BRSV, at the fifth passage level in cell culture, was administered by a combined respiratory tract route (intranasal and intratracheal) for four consecutive days. Four groups of calves were utilized as follows: Group I, 6 calves sham inoculated with uninfected tissue culture fluid and necropsied 21 days after the last inoculation; Group II, 6 calves inoculated with BRSV and necropsied at the time of maximal clinical response (4-6 days after the last inoculation); Group III, 6 calves inoculated with BRSV and necropsied at 21 days after the last inoculation; Group IV, 6 calves inoculated with BRSV, rechallenged with BRSV 10 days after initial exposure, and necropsied at 21 days after the initial inoculation. Clinical response was evaluated by daily monitoring of body temperature, heart rate, respiratory rate, arterial blood gas tensions, hematocrit, total protein, white blood cell count, and fibrinogen. Calves were necropsied and pulmonary surface lesions were quantitated by computer digitization. Viral pneumonia was reporduced in each principal group. Lesions were most extensive in Group II. Disease was not apparent in Group I (controls). Significant differences (p less than 0.05) in body temperature, heart rate, respiratory rate, arterial oxygen tension, and pneumonic surface area were demonstrated between control and infected calves. Results indicate that severe disease and lesions can be induced by BRSV in one-month-old calves that were colostrum-fed and seropositive to BRSV. BRSV rechallenge had minimal effect on disease progression. Based on clinical and pathological response, results did not support viral hypersensitivity or persistent infection as pathogenetic mechanisms of BRSV pneumonia.
Animals↗
The role of passive immunity in bovine respiratory syncytial virus-infected calves.
The role of passive immunity in bovine respiratory syncytial virus (BRSV) infections in neonatal calves was evaluated. Calves were divided into groups as follows: colostrum-deprived, sham-inoculated; colostrum-deprived, BRSV-inoculated; and colostrum-fed, BRSV-inoculated. Calves were inoculated with a low-passage field isolate of BRSV for 4 consecutive days by a combined respiratory tract route and were euthanized 6 days after receiving the last inoculation. Arterial oxygen tension (Pao2) decreased significantly over time in colostrum-deprived, BRSV-inoculated calves (P less than .01) and was significantly different among treatment groups (P less than .05). A significant decrease in arterial oxygen saturation was observed in this same group over time (P less than .01). Mean percentage of pneumonic lung volume (determined by computer data digitalization) was significantly greater in infected, colostrum-deprived calves compared with the other groups (P less than .01), and BRSV antigen was detected in these calves by avidin-biotin immunoperoxidase staining. Thus, passive immunity derived from colostrum feeding decreased the severity of BRSV infections in calves.
Analysis of Variance↗
Systemic lupus erythematosus in a filly.
Systemic lupus erythematosus (SLE) was diagnosed in a 2-year-old Standardbred filly. Clinical signs of SLE included weight loss, bilateral symmetric alopecia, seborrhea, oral ulceration, and lymphadenopathy. Abnormal laboratory findings included a Coombs test-positive hemolytic anemia and positive antinuclear antibody test result. Histologic evaluation of multiple skin biopsy specimens revealed interface dermatitis with linear deposition of IgG at the basement membrane zones of the epidermis and hair follicles. The filly did not respond to glucocorticoid treatment and was euthanatized. Necropsy findings included membranous glomerulonephritis and fibrous synovitis. On the basis of these findings, SLE should be considered in the differential diagnosis of immune-mediated skin disease in horses. Definitive diagnosis of SLE relies on recognition of multisystemic disease and confirmatory histopathologic and immunopathologic findings.
Animals↗
Idiopathic granulomatous disease involving the skin in a horse.
Clinical signs of generalized granulomatous disease in a horse included depression, generalized lymphadenopathy, scaly skin, and dependent edema. Diagnosis was confirmed histopathologically by diffuse granulomas in more than one organ system (lymph nodes and skin), and by ruling out etiologic agents. Response to treatment with corticosteroids was favorable. The clinical features and response to treatment in this horse were different from classic textbook descriptions of equine generalized granulomatous disease. Equine granulomatous disease appears to be comparable to human sarcoidosis. To avoid confusion between human medical and veterinary medical terminology, the authors suggest that the equine disease be referred to as equine idiopathic granulomatous disease and that a complete diagnostic description should include a reference to the organ systems involved.
Animals↗
The identification of maternity in an unusual pregnancy-related homicide.
A recent bizarre homicide which culminated in the delivery of a live-born infant necessitated careful determination of true maternal origin. A 23-year-old pregnant woman was abducted, subdued, strangled, and delivered of a term infant by a crude Cesarean section. The infant was stolen and subsequently presented to physicians by a woman posing as the mother. Methods used to help confirm the surviving infant's parentage involved red cell antigen and enzyme system evaluations as well as immunoglobulin allotyping, which ultimately proved to be the most effective serologic test performed. The forensic science investigation of this unusual case also used bite mark analysis and patterned injury interpretation. Immunoglobulin allotyping is specifically discussed as a forensic serology test which is currently available and particularly applicable in cases involving parentage determination.
ABO Blood-Group System↗
Congenital central diabetes insipidus in two sibling Afghan hound pups.
Congenital central diabetes insipidus was determined to be the cause of polydipsia and polyuria in sibling pups. Both pups were lacking adequate plasma arginine vasopressin concentration, compared with that in control dogs. Microscopic abnormalities were confined to the brain and pituitary gland in one pup. Without breeding trials of these dogs or their relatives, it cannot be determined whether the cause was familial.
Animals↗