PubMed Health⌕ Search

Biomedical subjects

E G Kokko

Publications and source records attributed to E G Kokko.

3 recordsLinked to original sources
PubMed1977

Orseillin BB and crystal violet, a differential staining combination for paraffin sections and water mounts of fungi.

A saturated solution of orseillin BB in 3% acetic acid followed by a 1% aqueous solution of crystal violet provides an excellent differential staining for sections of ascomycetous fructifications. The technique stains a wide variety of fungus and host cells, revealing considerable morphological and cytological detail. It is appropriate for microscope slides both of unfixed material mounted in water and of picric acid-fixed paraffin sections.

Acetates↗
PubMed1976

The ultrastructure of the Spilocaea state of Venturia inaequalis in vivo.

There are indications that the fungus enzymatically degrades the cuticle and epidermal cell wall. The epidermal cells and to a lesser degree the palisade mesophyll cells beneath a sporulating lesion (susceptible reaction) are killed or seriously disrupted. Various stages of conidiogenesis, including development of the pprimary conidium, were observed. A conidium is delimited by a two-layered transverse septum. Before condium secession, a new two-layered inner wall is laid down around the entire conidiogenous cell adjacent to the plasmalemma. The apical region of the new inner wall proliferates beyond the annellation scar left by the seceded conidium and eventually produces another conidium.

Ascomycota↗