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E G Volina

Publications and source records attributed to E G Volina.

At least 19 recordsLinked to original sources
PubMed1990

[A comparative evaluation of the sensitivity of different methods for the serological diagnosis of leptospirosis].

In this work the comparative evaluation of the sensitivity and serological specificity of the microcapsular agglutination (MCA) test, the passive hemagglutination (PHA) test and the microagglutination (MA) test are presented. In the MCA test leptospiral antigens, adsorbed on synthetic carrier capsules produced by Japan Lyophilization Laboratory, were used and the PHA test was made with the use of polyvalent erythrocyte diagnosticum. The study of blood serum samples from 46 leptospirosis patients revealed that the values of antibody titers in the PHA and MCA tests were 5.5-8.1 times higher than the traditional MA test. In the MCA and PHA tests antileptospiral antibodies could be detected as early as on days 1-3 of the disease when the results of the MA test were negative or very low. The maximum values of antibody titers in the MCA and PHA tests were detected on days 11-15 of the disease and in the MA test, on days 21-25. The MCA and PHA tests are genus-specific and permit the detection of antileptospiral antibodies irrespective of the serogroup of the infective agent. In the study of the blood sera of 40 patients with diseases of nonleptospiral etiology the MCA and MA tests yielded false positive results in 7.5% and the PHA test, in 12.5% of cases in titers below the diagnostic level. These data are indicative of high sensitivity and specificity of the serological tests used in this study.

Agglutination Tests↗
PubMed1988

[Use of microcapsular leptospiral antigens for detecting specific antibodies].

The sensitivity of microcapsular leptospiral antigens, produced by Japan Lyophilization Laboratory and intended for use in tests for the detection of antibodies to leptospires in the sera of experimentally immunized laboratory animals, were studied. The comparative study of the microcapsular agglutination (MCA) test and other serological tests, such as the microagglutination (MA) test and the indirect enzyme immunoassay (EIA), was made. The leptospiral antigens under study were found to actively react with serospecific and group-specific antibodies. In infected guinea pigs and rabbits specific antibodies could be detected from days 3-4 in the MCA test and only from days 5-7 in the MA test. The average antibody level determined by titration in the MCA test was 3.3 times higher and in indirect EIA, 4.3 times higher than that determined by titration in the MA test. These data make it possible to recommend the use of microcapsular leptospiral antigens for the early diagnosis of leptospirosis.

Adsorption↗
PubMed1988

Investigation of hemolytic activity of leptospirae on solid culture media.

Results of investigation of hemolytic activity of leptospirae against red blood cells of various animal species on solid culture media using the technique of agar layers are presented. It has been shown that hemolytic properties of pathogenic and saprophytic leptospirae differ with respect to red blood cells of the sheep, the rabbit, the hamster, the albino rat and the albino mouse. Hemolytic activity of leptospirae regarding the erythrocytes of the cattle, the horse and the fowl depends on the strain of leptospirae. The advantage of using solid nutrient media for the determination of hemolytic properties of leptospirae in comparison with liquid media has been demonstrated.

Animals↗
PubMed1986

[The use of direct immunoenzyme analysis with genus-specific peroxidase conjugate in leptospirosis].

For the first time anti-Patoc I peroxidase conjugate was obtained from the serum of a hyperimmunized guinea pig by the method of Nakane and Kawaoi (1974) in the modification of Mathiesen et al. (1978). In the experimental study of 37 Leptospira strains belonging to 19 serological groups, carried out with the use of direct EIA techniques, anti-Patoc I peroxidase conjugate was found to be genus-specific and highly active. The possibility of detecting Leptospira antigens of 7 serological groups in the blood and in suspensions of the liver and kidneys of golden hamsters by direct EIA with the use of the above-mentioned conjugate was established. The use of direct EIA with anti-Patoc I peroxidase conjugate for the diagnosis of leptospirosis is recommended.

Animals↗
PubMed1986

Phospholipase activity and virulence of pathogenic leptospirae.

Results of investigation of phospholipase activity and virulence of pathogenic leptospirae on a solid nutrient medium using the method of agar layers with 1% of L-lecithin in the medium of the second layer are presented. It has been demonstrated that only one zone of translucent medium is formed around the colonies of pathological leptospirae, which is obviously due to the release of phospholipase A. The examined virulent strains of pathogenic leptospirae were found to exhibit greater phospholipase activity (width of the translucent zones being 5.0 +/- 0.34 mm) than the avirulent strains (width of the zones being 1.5 +/- 0.11 mm).

Agar↗
PubMed1983

[Comparative study of the phospholipase activity of pathogenic and saprophytic Leptospira cultured on a serum-lecithin agar].

The phospholipase activity of leptospires cultivated on serum-lecithin agar has been studied. Two zones of changes in the medium have been found to appear around the colonies of saprophytic Leptospira strains: transparent (5.25 +/- 2.09 mm wide) and turbid (6.90 +/- +/- 1.46 mm wide), which is linked with the production of phospholipases A and C. Only a single clear zone is formed around the colonies of pathogenic strains due to the production of phospholipase A. At the same time virulent Leptospira strains show greater phospholipase activity (the zones are 6.0 +/- 1.2 mm wide) than avirulent strains (the zones are 1.6 +/- +/- 0.04 mm wide).

Agar↗
PubMed1979

[Dynamics of Leptospira colony formation on solid nutrient media].

The formation of Leptospira colonies on solid culture media varying in composition were studied. In all species of Leptospira 3 main periods of colony development were revealed, each of them having its characteristic morphological changes connected with the growth of the colony deeply into agar.

Culture Media↗
PubMedDate not supplied

[Cultivation of Leptospira in a liquid culture medium with lysed rabbit blood].

A new method of the preparation of liquid culture medium for the cultivation of Leptospira has been developed on the basis of multiple freezing and thawing of defibrinated rabbit blood. A higher productivity of the new medium in comparison with the medium used in common practice has been established. The medium having a new composition is suitable for cultivation of Leptospira and the accumulation of biomass. The advantage of the medium made is in addition to high growth properties, the its economic efficiency and availability for practical laboratories.

Animals↗
PubMedDate not supplied

[Detection of anti-Leptospira antibodies in sera of patients in the latex agglutination test].

The results of the preliminary evaluation of the sensitivity and specificity of the newly developed diagnostic test based on the determination of genus-specific antibodies to leptospires in the latex agglutination test, are presented. This test makes it possible to detect anti-Leptospira antibodies of any serogroup. The advantages of the developed test have been determined.

Antibodies, Bacterial↗