PubMed Health⌕ Search

Biomedical subjects

E Gattavecchia

Publications and source records attributed to E Gattavecchia.

28 records · Page 2Linked to original sources
PubMed1982

Thin-layer chromatographic determination of indolic tryptophan metabolites in human urine using Sep-Pak C18 extraction.

Tryptophan and some of its indole metabolites were separated by thin-layer chromatography, stained with the Van Urk--Salkowski reagent, and quantitated by scanning densitometry. The application of this technique for the detection of the indoles in urine samples, employing Sep-Pak C18 cartridges for extraction, was demonstrated. The proposed method is simple and accurate. The detection limits were 2 micrograms/ml 5-hydroxytryptophan, 1.75 micrograms/ml 5-hydroxyindolyl-3-acetic acid, 1.5 micrograms/ml tryptophan, 0.8 micrograms/ml indolyl-3-acetic acid, 0.9 micrograms/ml indolyl-3-butyric acid, 1.75 micrograms/ml serotonin, and 1.25 micrograms/ml tryptamine.

Chromatography, Thin Layer↗
PubMed1981

Effect of diflubenzuron and its major degradation products on the growth of Euglena gracilis Z. and incorporation of glycine-U-14C in protein.

Diflubenzuron (I) and its major degradation products 4-chlorophenyl urea (II), 2,6-difluorobenzoic acid (III) and 4-chloroaniline (IV) were tested for their activity on Euglena gracilis Z. The inhibition on the growth and on the incorporation of glycine-U-14C in the protein of Euglena was measured in the presence of I-IV ranging 10 to 200 ppm. 4-chloroaniline caused a considerable inhibition at every tested level whereas I-III slightly affected only the incorporation. Therefore, it must be inferred that diflubenzuron shows no effect on growth and protein biosynthesis for this nontarget organism.

Carbon Radioisotopes↗
PubMed1977

Radiobichemistry of phytodrugs: II. Activity of Altoside (r) and Altozar (R) in the biosynthesis of proteins and RNA in larvae of shrimps in vivo studied by leucine-U-14C and uridine-2-14C.

The present communication concerns the investigation of the effect of various concentrations of Altosid (R), J.H.A., on the biosynthesis of proteins and RNA in cultures of shrimp larvae in vivo measured by the uptake up Leucine, U-14C and Uridine-2-14C. The incorporation of Leucine follows different behaviour in the presence of either 0.01 or 1 ppm concentration of J.H.A.. At any dose Altosid (R) and Altozar (R) decrease the protein synthesis in the larvae of shrimps also at concentrations of 0.01 ppm, the main dose used for their application. In fact in these condition the synthesis of proteins is lower than in the control. For the RNA biosynthesis the uptake of Uridine is much higher in the presence of J.H.A. than in the control. It is suggested then an abnormal s-RNA is formed in the first hours, which does not synthetize proteins.

Animals↗
PubMed1976

Radiobiochemistry of phytodrugs: I. role of juvenile hormones and analogs in the biosynthesis of proteins and RNA in drosophila larvae.

The present communication concerns the investigations of the effect of Juvenile Hormone (J.H.) and its analogs (J.H.A.), such as Altosid (R) and Altozar(R), used as insecticides, on the biosynthesis of proteins and RNA in subcellular preparations of drosophila larvae with Leucine-U-14C and Uridine-2-14C respectively. The incorporation of leucine into proteins decreases progressively from crude homogenate to the microsome-free fraction in the same way as the degradation of the compounds. During RNA biosynthesis the incorporation of uridine increases from the crude homogenate up to mithocondria-free fraction and with Altosid is always below the test controls. The reduced incorporation of uridine into RNA and leucine into proteins in the presence of Altosid and Altozar indicates a genetic action of this compounds comparable to that of natural J.H. but slightly more efficient.

Animals↗