[Physiological importance and action of prostaglandins in reproduction of livestock].
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Biomedical subjects
Publications and source records attributed to E Grün.
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The response to transport stress of serum GOT, LDH, and ALD was studied in 35 heads of cattle, aged 18 months to 20 years. The influence of different factors upon serum enzyme behaviour in cattle after exposure to transport stress was tested by means of mathematico-statistical methods. The following conclusions were drawn: 1. Transport stress on cattle was followed by rise in GOT, LDH, and ALD activities in serum. 2. The strongest rise was recorded from ALD activity and the smallest from LDH. 3. All three serum enzymes continued to rise up to the time of slaughter (20 to 50 hours after transport). 4. The enzyme activities recorded from blood after slaughter did not differ significantly from those in venous blood. 5. Intensity of ALD activity rise was found to depend on the transport distance (if it was more than 20 km), age of animal, and ambient temperature. 6. The rise in GOT activity was found to depend only on the given ambient temperature. 7. The behaviour of LDH activity did not reveal any secured relationship with any of the examined factors (transport distance, age keeping, and tuberculosis state or ambient temperature).
To find out optimal measuring conditions for determination of G-6-PDH-activity in erythrocytes of cattle and pigs by means of the optical test the dependence from nature, concentration and pH of buffer surroundings as well as from concentration of substrate (G-6-P), coenzyme (NADP) and activator (Mg++) was studied. From the results obtained, best reaction conditions for determination of G-6-PDH-activity in this species exist taking the following composition of the measuring sample (final concentration): 120 mM TRIS/HCl-buffer, pH 9.0; 10 mM MgSO4; 2 mM G-6-P; 0.2 mM NADP and 0.1 ml haemolysate in 3.0 ml of the measuring volume. The influence of an optimal planning of the reaction conditions on registration of G-6-PDH-activity in erythrocytes of the farming animals is discussed.
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At male deer in the age of 15 and 27 months, at experimentally induced cryptorchids and at castrated animals analyses of the content of Na, K, Ca, Mg, Fe, Cu and Zn in 13 different tissues were performed. In the liver of newborn animals solely the content of Fe, Cu and Zn was determined. The significance of the content of the mentioned elements in the various tissues is described. In the liver of the newborn animals stores of Fe, Cu an Zn were identified. The values can be used as a basis for analyses at deer with a growth inhibition caused by a lack of sodium chloride Fe, Cu and Zn.