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Biomedical subjects

E H Bohl

Publications and source records attributed to E H Bohl.

51 records · Page 3Linked to original sources
PubMed1968

A buffy coat stimulating effect in serum from cattle with persistent lymphocytosis.

A stimulating effect on bovine buffy coat cells was demonstrated in blood samples from cattle with persistent lymphocytosis. The stimulation was a function of the serum and not the cells used. It was not altered by filtration or inactivation of the serum. When buffy coat cells were grown in homologous serum, the resulting growth was moderate to abundant when the donor animal had persistent lymphocytosis and was usually poor when the donor animal had a consistently normal hemogram.

Animals↗
PubMed1968

Some studies of infectious bovine rhinotracheitis (IBR) virus infection in calves.

Six dairy calves, six and one-half to nine months old, were exposed to a strain of infectious bovine rhinotracheitis (IBR) virus of bovine fetal origin by one of the various routes - nasal, vaginal, preputial or contact. Neither after initial exposure nor following challenge of their immunity did any of these animals manifest the IBR respiratory syndrome, although two of them (inoculated per vagina/prepuce) developed pustular vulvovaginitis or balanoposthitis. Also, one five-day old dairy calf which had received colostrum and milk of its IBR-immune dam, was inoculated intranasally with the same strain of IBR virus. This animal exhibited severe signs of IBR. The virus was recovered from all but three of the seven calves after initial exposure and from all but one animal following challenge of their immunity. Immune responses of these calves resembled those of adult cattle.

Animals↗
PubMed1966

Studies of Escherichia coli in gnotobiotic pigs. I. Experimental reproduction of colibacillosis.

The successful experimental production of colibacillosis in young gnotobiotic pigs by the oral administration of E. COLI (08:K.:H21 was accomplished. Colibacillosis was not produced in gnotobiotic pigs infected with either of the 2 other strains of E.COLI employed.Necropsy observations were presented and discussed. Emphasis was placed on the absence of grossly visible enteritis in pigs that died in the acute phase of the disease. The gross appearance of the intestinal tract changed some hours after death to resemble that associated with enteritis.Bacteremia was detected in the gnotobiotic pigs orally infected with each of the 3 strains of E. COLI, and the special characteristics of the colostrum-deprived pig were stressed as important factors in these findings.

Animals↗
PubMed1966

Studies of escherichia coli in gnotobiotic pigs. II. The immune response.

The immune response of orally infected and parenterally vaccinated (E. COLI 08:K.:H21) gnotobiotic pigs was studied by bactericidal and indirect hemagglutination test methods. The bacterial agglutination test proved to be of no value in this study. The results demonstrated that antibody was detectable within 8 days following oral infection, but the titers remained very low until after the pigs were vaccinated intravenously. The titers of the sera were markedly increased following intravenous vaccination. There was no detectable absorption of antibody from the gut of gnotobiotic pigs fed immune serum at 4 to 6 days of age and no detectable difference between the immune response of these pigs and those not fed serum.

Agglutination Tests↗
PubMed1966

Studies of Escherichia coli in gnotobiotic pigs 3. Evaluation of orally administered specific antisera.

The protective effect of orally administered immune (E. COLI 08:K.:H21) serum was demonstrated in experimentally infected gnotobiotic pigs. The temporary protective effect of the immune serum and the correlation of protection with IN VITRO antibody determinations were discussed. The results indicated that the protective action of the immune serum is apparently not dependent upon the complement-antibody bactericidal system. It was suggested that the most plausible mechanism of action of the immune serum was the inactivation of endotoxin in the lumen of the intestine.

Animals↗