Typhlocolitis caused by Clostridium difficile in suckling piglets.
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Biomedical subjects
E H Waters
Publications and source records attributed to E H Waters.
Immunohistochemical detection of swine influenza A virus in formalin-fixed and paraffin-embedded tissues.
An avidin-biotin complex (ABC) immunohistochemical method utilizing a commercially-available polyclonal antiserum to human influenza A virus was used to detect antigens of influenza A virus in formalin-fixed, paraffin-embedded tissues of swine. Influenza A antigens were immunohistochemically detected in 28/30 cases in which influenza A virus was demonstrated by virus isolation and in 5/22 cases suspected to be influenza A-infected by clinical and histological criteria, but from which the virus was not isolated. Viral antigen was not demonstrated in 30/30 cases not suspected clinically or histologically to be associated with influenza infection. This method is a convenient, sensitive, and specific means of influenza A virus detection and is applicable to both routine diagnosis of influenza A virus infection and to retrospective and prospective studies of the occurrence and the pathogenesis of this virus in pigs.
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Submandibular and disseminated zygomycosis (mucormycosis) in feeder pigs.
Submandibular and/or disseminated zygomycosis (mucormycosis) was diagnosed in 3 feeder pigs from 2 farrow-to-finish farms. Affected pigs were stunted and unthrifty. Each pig had a large unilateral submandibular granuloma replacing the mandibular lymph node on the affected side. Two pigs had disseminated lesions. One had an abscess on the serosa of the stomach; another had abscesses scattered throughout the liver and mesenteric lymph nodes. Impression smears were made of several masses and stained with blue-black ink. Examination of smears by light microscopy revealed coarse, infrequently septate, irregularly branching, mucoraceous fungi typical of a Zygomycete. Histologic examination of the submandibular masses revealed granulomas containing large numbers of mixed inflammatory cells, including giant cells. Mesenteric lymph nodes and liver abscesses had necrotic cores surrounded by fibrous capsules infiltrated by mixed inflammatory cells. Hyphae of a mucoraceous fungus were scattered throughout all masses. The 2 pigs submitted alive had marked serum hypoproteinemia, including hypoglobulinemia.
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