Biomedical subjects

E H van Vulpen

Publications and source records attributed to E H van Vulpen.

4 recordsLinked to original sources

PubMed1999

Hypothalamic A14 and A15 catecholamine cells provide the dopaminergic innervation to the supraoptic nucleus in rat: a combined retrograde tracer and immunohistochemical study.

This study investigated the origin of a dopaminergic innervation of the hypothalamic supraoptic nucleus. In pentobarbital-anaesthetized male Long-Evans rats, a transpharyngeal approach was used to inject a retrograde tracer, rhodamine latex microspheres, into the supraoptic nucleus. After 13-26 h survival under anaesthesia, animals were perfused transcardially, the brain sectioned and processed for tyrosine hydroxylase immunofluorescence, a marker for hypothalamic dopaminergic neurons. In six cases with injections restricted to the supraoptic nucleus, rhodamine-labelled microspheres were observed in a population of tyrosine hydroxylase-positive neurons located in the A15 cells below the anterior commissure (A15 dorsal) and above the optic chiasm (A15 ventral), and the dorsal and lateral periventricular A14 cell group. Occasional double-labelled cells were seen in the medial and lateral hypothalamus and bed nucleus of the stria terminalis, but rarely in other known dopaminergic cell groups, notably the ventral tegmental area (A10), zona incerta (A13) and substantia nigra. In support of a role for dopamine in neurohypophysial regulation, these observations indicate that the major dopaminergic input to magnocellular neurons in the hypothalamic supraoptic nucleus is derived from a relatively sparse population of neurons located in the A14 and A15 cell groups.

Animals↗

PubMed1998

Striatal cholinergic interneurons: birthdates predict compartmental localization.

The striatal patch and matrix compartment neurons are born at different times during rat development. The majority of the early born neurons preferentially end up in the patch compartment, while the majority of the later born neurons end up in the matrix compartment. Although the cholinergic interneurons are all born early in neurogenesis (between embryonic day E12 and E17), and we would therefore expect them to be located mainly in the patches, they are relatively homogeneously distributed in the adult, with a preference for the matrix area just outside the patches (the intermediate zone). To ask if birthdate can predict the compartmental localization of cholinergic neurons in the striatum, we marked new postmitotic neurons in the embryo with a maternal injection of bromodeoxyuridine (BrdU) on E13, E15 or E17 and labeled the patch compartment with an injection of the retrograde tracer True Blue into the substantia nigra on postnatal day (P) 1. The pups were sacrificed at P40 and the tissue was processed for BrdU, choline acetyltransferase, and True Blue triple labeling. Cholinergic neurons that became postmitotic at E13, had a higher chance of ending up in the patch compartment compared to either the intermediate zone or the rest of the matrix compartment. On the other hand cholinergic neurons that became postmitotic at E17 had a higher chance of ending up in the matrix compartment (including the intermediate zone). We conclude that birthdate can predict compartmental localization, with the cholinergic neurons in the intermediate zone following the same pattern as the cholinergic neurons in the rest of the matrix compartment. Cholinergic neurons show the same relative birthdate/compartment relationship as do other striatal neurons, although the absolute birthdates of cholinergic neurons are shifted earlier in neurogenesis.

Acetylcholine↗

PubMed1989

Organization of projections from the mediodorsal nucleus of the thalamus to the basolateral complex of the amygdala in the rat.

Mediodorsal thalamic (MD) projections to the basolateral amygdaloid complex of the rat were investigated with the anterograde neuronal tracer, Phaseolus vulgaris-leucoagglutinin. Iontophoretic injections were made in distinct subdivisions of the rostral and caudal part of the MD. Both the medial and lateral division of the MD showed a projection to the basolateral complex and there appears to be a topographical organization of the innervation in the rostrocaudal direction: the rostral and caudal part of the MD project to respectively the mid-rostrocaudal and rostral part of the basolateral complex.

Amygdala↗

PubMed1987

Ultrastructural localization of GABA in the supraoptic nucleus and neural lobe.

Antibodies directed against the neurotransmitter gamma-aminobutyric acid (GABA) enabled the ultrastructural localization of GABA in conventional glutaraldehyde fixed and osmium postfixed material of the rat supraoptic nucleus and neural lobe. GABA was visualized using immunogold postembedding staining in axonal profiles that terminate on dendrites, axons or cell bodies throughout the supraoptic nucleus. The optimum ultrastructural preservation made possible the visualization of GABA terminals, also in the neural lobe. Here GABA axons were found to terminate synaptically on pituicytes and axonal profiles containing large dense core vesicles. These results emphasize, from an anatomical point of view, the potency of GABA to influence, as a transmitter, the release of vasopressin and oxytocin, both at the level of the cell body and of the neural lobe.

Animals↗