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Biomedical subjects

E Hadas

Publications and source records attributed to E Hadas.

At least 37 records · Page 2Linked to original sources
PubMed1996

Immunisation of sheep by drug-abbreviated infections of Ostertagia circumcincta and Trichostrongylus colubriformis against field challenge of gastro-intestinal nematodes.

A very high level of protection was achieved against homologous (up to 97%) and heterologous (up to 87%) infections in 12-month-old Romney sheep immunised with oxfendazole-abbreviated infections of Ostertagia circumcincta and Trichostrongylus colubriformis. No significant protection occurred following ivermectin-abbreviated infections. None of the immunised sheep showed an increase in antibody level against excretory-secretory antigen of T. colubriformis infective larvae. The immunisation procedures did not cause a decrease in wool production, or liveweight gains compared with non-immunised controls.

Animal Feed↗
PubMed1996

Supravital staining of eosinophils.

Live eosinophils when mixed with Acridine Orange solution and viewed microscopically using u.v. light show very intense colours of their granules (yellow, orange and red) and green nuclear staining. Their active movement, translocation of granules and degranulation can be observed in vitro. Using this method, live eosinophils can be easily differentiated and enumerated.

Acridine Orange↗
PubMed1996

Field studies of the immunisation of lambs with drug-abbreviated infections of Trichostrongylus colubriformis and Ostertagia circumcincta.

Fifty, 5-6 month-old lambs were randomly allocated to five equal groups. Three groups of lambs were immunised by three oxfendazole-abbreviated (artificial) infections of Trichostrongylus colubriformis and Ostertagia circumcincta. Group 1 was immunised with a high dose of larvae, Group 2 with a medium dose and Group 3 with a low dose. A fourth group was treated with oxfendazole only and a fifth group was not treated (control). All groups were grazed together on pasture naturally contaminated with nematode larvae. Immunisation significantly reduced the number of eggs per gram of faeces in all three groups, but the lowest faecal egg count was seen in Group 3 (immunised with the lowest number of larvae). Significantly better liveweight gains and wool weight were observed in Group 2 animals than in the control group. Of all anthelmintic-treated animals, only Group 1 lambs did not have a significantly lower dag weight than controls.

Journal Article↗
PubMed1996

Sequential measurements of human decidua-associated protein (hDP) 200 in the uterine fluid during the menstrual cycle.

PROBLEM: To examine the relationship between the concentration of uterine fluid human decidua-associated protein (hDP) 200, identified as a monoclonal rheumatoid factor, and different phases of the menstrual cycle. METHODS: Sequential measurements of hDP 200 concentration in uterine fluid were performed in 11 normal ovulatory women, aged 22-36 years. The samples were collected in early proliferative phase, late proliferative phase, periovulatory period, early secretory phase, and late secretory phase. RESULTS: Consistent fluctuations of hDP 200 levels in uterine fluid were found throughout the menstrual cycle. High levels were found during early proliferative phase and periovulatory period related to significantly lower levels during late proliferative and early luteal phases. CONCLUSION: There is menstrual phase dependent variation in the uterine fluid levels of hDP 200.

Adult↗
PubMed1996

Abnormal uterine bleeding with normal uterine fluid level of human decidua-associated protein 200 reflects normal endometrial histology.

In the current study, the uterine fluid level of human decidua-associated protein (hDP) 200 was measured in 80 women with abnormal uterine bleeding and normal histology. In 20 women the level of hDP200 was also correlated with the endometrial thickness measured by transvaginal ultrasonography. The results demonstrated the same distribution and similar levels of uterine fluid hDP200 throughout the menstrual cycle as in regularly cycling women, with no correlation between the levels of hDP200 and the endometrial thickness. Thus, the test for uterine fluid hDP200 could serve to discriminate between benign and pathological examinations of endometrial biopsies in women of reproductive age, women with abnormal uterine bleeding and benign conditions of the endometrium. Very low levels of hDP200 are indicative of endometrial hyperplasia or carcinoma.

Adult↗
PubMed1996

Immunosuppressive effect of uterine fluid on spermatozoa-stimulated mixed lymphocyte cultures.

Spermatozoa, obtained from 20 healthy individuals, were incubated with crude uterine fluid or with a purified IgG fraction obtained from uterine fluid before adding them to cultures with allogeneic lymphocytes. Untreated spermatozoa induced a proliferative 1.5- to 6-fold increase, while the spermatozoa treated with crude uterine fluid or with purified IgG fraction caused 84-100% suppression of lymphocyte blastogenesis. These results suggest the existence of an endogenous uterine fluid factor, apparently IgG, which causes inhibition of lymphocyte proliferation induced by spermatozoa in the uterine lumen.

Adult↗
PubMed1996

Rheumatoid factor-associated high-molecular-weight complexes in the menstrual and amniotic fluids.

We have previously described the identification of the human decidua-associated protein hDP200 as a rheumatoid factor [1]. Assuming that rheumatoid factor binds to Fc of immunoglobulins, the existence of high-molecular-weight complexes was studied in decidual extract, pooled uterine fluid from proliferative and early secretory phase, menstrual fluid and amniotic fluid. High-molecular-weight complexes containing hDP200 and other immunoglobulins were found only in menstrual and amniotic fluids and not in decidual extract or uterine fluid. It is possible that complexing of the immunoglobulins by hDP200 serves as one of the mechanisms that ensure suppression of the immune response to fetal antigens.

Adult↗
PubMed1995

Human decidua-associated protein hDP200 in menstrual fluid: comparison between fertile women and women after failed in-vitro fertilization/embryo transfer treatment.

The concentration of human decidua-associated protein (hDP) 200 was measured in 238 menstrual fluid samples obtained from normal fertile women and in 26 menstrual fluid samples obtained from infertile women who failed to conceive and menstruated following in-vitro fertilization (IVF)/embryo transfer treatment. A significant association was observed between the concentration of hDP200 and the age of the women. A maximal concentration of hDP200 was observed in women aged 28-30 years, with significantly lower concentrations in those aged 18-20 and 39-41 years. The presence of an intra-uterine device had no effect on menstrual fluid hDP200 concentrations. A significantly lower mean concentration of hDP200 (82 U/ml) was measured in menstrual fluid samples obtained from failed IVF/embryo transfer patients as compared with that in menstrual fluid samples from normal fertile women (191 U/ml). These findings support the concept that adequate endometrial function, as evaluated by menstrual fluid concentration of hDP200, is important for the success of the fertility process.

Adolescent↗
PubMed1995

Uterine fluid human decidua-associated protein 200 and implantation after embryo transfer.

Uterine fluid samples from 109 patients undergoing in-vitro fertilization and embryo transfer were obtained so as to examine the relationship between the uterine fluid concentration of human decidua-associated protein (hDP) 200 and the implantation rate. The sampling was performed on the day of embryo transfer with a Wallace catheter, used for the testing of cervical patency before embryo replacement. The implantation rate, as well as the pregnancy rate, demonstrated a significantly positive correlation with the concentration of hDP 200 in the uterine fluid, measured just before embryo transfer. These results indicate that hDP 200, identified as a rheumatoid factor secreted by the endometrium, may be involved in the implantation process.

Decidua↗
PubMed1995

The level of human decidua-associated protein hDP-200, in uterine fluid and serum.

Prospective uterine fluid sampling in 140 patients was performed using a Wallace catheter. The levels of human decidua-associated protein (hDP)-200 in the uterine fluid and in the serum, and the total protein concentration in the uterine fluid were determined. Uterine fluid volumes were scored during the hysteroscopy and no correlation was found with the phase of the menstrual cycle. The total protein concentration in the uterine fluid did not vary significantly during the menstrual cycle. The hDP-200 levels in uterine fluid were significantly higher than those in the serum, although considerable individual variation in the level of uterine fluid hDP-200 was found.

Body Fluids↗
PubMed1994

Human decidua-associated protein 200 levels in uterine fluid at hysteroscopy.

Hysterosocpic intrauterine findings and levels of human decidua-associated protein 200 (hDP 200) in the uterine fluid were recorded in 116 women investigated for infertility or recurrent abortions. The levels of hDP 200 were significantly higher in the presence of submucous myomas or endometrial polyps, and lower in the presence of intrauterine adhesions in comparison to those in normal uterine cavities. hDP 200, an immunoglobin secreted by the endometrium, may be involved in implantation and placentation and its level in the uterine cavity is influenced by the presence of intrauterine pathology.

Abortion, Habitual↗
PubMed1994

Human decidua-associated protein hDP200 appears to be a rheumatoid factor.

The amino acid sequences of the amino-terminal of the two different polypeptide chains of the human decidua-associated protein hDP200, with apparent molecular masses of 55 and 25 kDa, were determined. The amino-terminal sequence of the 55 kDa chain revealed that it is an immunoglobulin heavy chain, of the VH-III subgroup and the amino-terminal sequence of the 25 kDa chain showed that it is a human kappa V-III light chain. The sequence data indicate that hDP200 is an immunoglobulin. The ability of the hDP200 molecules to form high molecular weight complexes with immunoglobulins of other classes suggest that hDP200 is a rheumatoid factor.

Amino Acid Sequence↗
PubMed1993

Superoxide dismutase of Trichinella spiralis and Trichinella pseudospiralis larvae.

The aim of this study was to compare the in vitro and in vivo activities of superoxide dismutase, an enzyme acting against free-radicals, in Trichinella spiralis and T. pseudospiralis isolates. In vitro, the activity of the superoxide dismutase in T. pseudospiralis was determined to be about two times higher than in T. spiralis. Only one band representing superoxide dismutase was found by electrophoretic analysis. In vivo, in muscles infected with T. spiralis, a strong histochemical reaction in the capsule and the layer directly adhering to it was found. In muscles infected with T. pseudospiralis, the activity was most marked in the vicinity of the larva and becomes less marked with the distance. The character of the reaction indicated that the enzyme is excreted by the larvae.

Animals↗
PubMed1993

Proteolytic enzymes of pathogenic and non-pathogenic strains of Acanthamoeba spp.

The aim of this work was biochemical characterization and classification of proteinases in pathogenic and non-pathogenic strains of Acanthamoeba spp. The authors showed two proteinase (acid 35 kDa and alkaline 65 kDa) which could be separated electrophoretically. Acid proteinase was inhibited by serine proteinase inhibitors such as DIFP. The second enzyme which was active at alkaline pH, was enhanced by EDTA and inhibited by iodoacetate (IAA) and (p-CMB) p-chloromercuribenzoate. These substances are known to inhibit cysteine type proteinases. The alkaline proteinase was more distinctively active in pathogenic strains and belongs to cysteine class (EC 3.4.22), whereas the acid proteinase was similar active in pathogenic and non-pathogenic strains and belong to serine class (EC 3.4.21).

Acanthamoeba↗
PubMed1992

A rapid and sensitive heterogeneous immunoelectrochemical assay using disposable electrodes.

In this novel enzyme-tagged immuoelectrochemical assay, disposable carbon felt discs serve both as electrodes and as the heterogeneous solid phase. Antibodies are immobilized on the carbon felt via a diaminoalkane-biotin-avidin-biotin bridge. Alkaline phosphatase is used as a label. Bound antibodies are monitored by following the electro-oxidation of aminophenol, produced enzymatically from p-amino-phenyl phosphate by the immobilized alkaline phosphatase at the electrode surface. A model system designed for determination of mouse IgG concentration yielded a calibration curve ranging from 10 pg/ml to 100 micrograms/ml. This assay can be performed rapidly and a single determination completed within 20 minutes. The system is useful also for rapid quantitation of a small number (approximately 80 organisms per ml) of bacteria.

Animals↗
PubMed1992

[The effect of fluoridated milk on caries reduction over 12 years].

The aim of the study was to assess the caries-preventive effect of fluoridated milk after 12-years of consumption, including 2 years interruption. DMF mean values of 162 institutionalized children aged 7-to 14 years were compared with DMF mean values of 139 institutionalized control children of the same age. The children consumed 200 ml milk daily, implemented with 0.4 mg -0.75 mg F(-)--depending on their age--between 1979-1984, after 2 years interruption they got fluoridated milk or cocoa-milk twice a week during 10 years. In the test group the ratio of caries-free children averaged more than 10%. The caries prevalence in the permanent dentition showed statistically significant reduction. The caries reduction regarding all groups was 36.78% in case of DMF-T, and 40.02% of DMF-S. According to our results the consumption of fluoridated milk in institutionalized children proved to be effective also in the long run.

Adolescent↗
PubMed1992

Effect of fluoridated milk on caries: 10-year results.

The aim of the study was to assess the caries-preventive effect of fluoridated milk after 10 years of consumption, including a two-year interruption. DMF mean values of 162 institutionalized children aged 7- to 14-years were compared to DMF mean values of 139 institutionalized children (control) of the same age. The test group children consumed 200 ml milk daily, implemented with 0.4 mg-to 0.75 mg F, depending on their age, continuously for six years. Following a two year interruption, the children again got fluoridated milk or cocoa-milk twice a week for four years. In the test group the average ratio of caries-free children was 10% more than in the control group. The caries prevalence in the permanent dentition showed a clinically and statistically significant reduction: 36.78% on DMFT, and 40.02% on DMFS mean values.

Adolescent↗