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Biomedical subjects

E Hai

Publications and source records attributed to E Hai.

5 recordsLinked to original sources
PubMed2003

Mast cell chymase expression in Helicobacter pylori-associated gastritis.

AIMS: To study the role of mast cell chymase in the inflammatory processes of human chronic gastritis. Experimental studies have shown that mast cell chymase stimulates inflammatory cell accumulation, and contributes to angiotensin II formation. METHODS AND RESULTS: Tissue sections from human stomachs with Helicobacter pylori-associated gastritis (surgery/autopsy n = 20; biopsy n = 16) and normal stomachs (n = 10) were studied using immunohistochemical single and double labelling techniques. Monoclonal antibodies used were directed against mast cell chymase, tryptase, neutrophils (CD66b, elastase, and myeloperoxidase), macrophages, T-lymphocytes, and interleukin (IL)-4. The expression of angiotensin-converting enzyme and angiotensin II type 1 receptor was investigated using immunohistochemical analysis and the reverse transcription-polymerase chain reaction. The number of chymase-positive mast cells was significantly higher (P < 0.0001) in H. pylori-associated gastritis than in normal stomachs. Increased expression of chymase in inflamed mucosa was closely related to an increase in the accumulation of neutrophils, macrophages, T-lymphocytes, and IL-4-positive cells. The expression of angiotensin-converting enzyme and angiotensin II type 1 receptor was not altered in gastritis specimens. CONCLUSIONS: These observations suggest that mast cell chymase may be an important mediator in the inflammatory processes of human H. pylori-associated gastritis.

Chronic Disease↗
PubMed2003

Hepatitis C virus genotype testing in paraffin wax embedded liver biopsies for specimen identification.

Despite advances in medical technology, careful specimen identification is still a fundamental principle of laboratory testing. If pathological samples are mixed up, especially in the case of extremely small biopsy samples, large amounts of time and energy may be wasted in correctly identifying the specimens. Recently, two liver biopsy specimens were mixed up in this department, and a new pathological technology was used to resolve the issue. Liver biopsy was performed on two patients with hepatitis C virus (HCV) infection. During sample transfer or tissue processing, the biopsy specimens were mixed up. Because the ABO blood group of the two patients was identical (type AB), the specimens were subsequently identified by analysing the HCV genotypes. RNA extracted from the paraffin wax embedded liver specimens was examined by a polymerase chain reaction based HCV genotype assay. This enabled the correct identification of the specimens, and each patient received the appropriate treatment on the basis of the accurate diagnosis.

Adult↗
PubMed2002

[Clinical study of double primary cancer involving the lung in resected cases].

Among all cases of surgically resected lung cancer, there were 56 cases (16.1%) of double primary cancer. The common sites of the other primary cancer was the stomach (19 cases), followed by large intestine (9 cases), urinary bladder (7 cases) and pharinx-larynx (7 cases). One patient had triple cancers. In all cases of double primary cancer, 46 cases were metachronous, 10 of which were cases of initial lung cancer. The 5-year survival rate of double primary cancer was 39.7%. Good result was obtained in metachronous cases with initial lung cancer. Most of prognosis of double primary cancer was determined by that of lung cancer. In more than half of initial cancer, the second primary cancer was detected by symptoms. So, special attention to the possibility of double primary cancer in patients with resected lung cancer is necessary for improvement of prognosis.

Aged↗
PubMed1986

Tension headache treated by anti-inflammatory drug injected into GB 20 acupuncture point.

The anti-inflammatory drug Voltaren (0-(36-dichlorophenyl)-amino acetate) is a relatively new substance in the systemic treatment of various pain inducing diseases related to the musculoskeletal elements. In order to achieve effective results, relatively high dosage, applied P.O.P.R. or I.M. should be used for prolonged periods. The acupuncture methods presented the eastern minded western physician with a detailed list of trigger points which may have abrogated certain painful symptoms when appropriately used by acupuncture maneuvers alone. In the present study the possibility of using the appropriate acupuncture point GB 20 for the injection of Voltaren, in an attempt to relieve stable and prolonged tension headaches, has been considered. Thus, acceptable and combined eastern-western treatment method, if found successful, may be evolved. GB 20 point has been selected because of its effective influence among others, on tension headaches, a highly common and most distressing syndrome poorly treated by conventional approaches in most clinics.

Acupuncture Therapy↗