PubMed Health⌕ Search

Biomedical subjects

E Haneke

Publications and source records attributed to E Haneke.

At least 19 recordsLinked to original sources
PubMed1991

[Delayed hypersensitivity to protamine and immediate hypersensitivity to insulin].

A 63-year-old female, with type II diabetes mellitus, diagnosed in 1967, was started on combination therapy with sulphonylureas and human depot insulin in May 1989, because of inadequate blood sugar control with sulphonylureas alone. Within 3 months she began to develop nodular skin reactions at the site of injection, 12-24 hours after insulin injections. Intradermal testing demonstrated delayed (Gell and Coombs type IV) hypersensitivity to protamine. No specific IgE or IgG antibodies were demonstrable. She was changed to protamine-free human delayed action insulin. After an initial reaction-free period, red urticarial lesions, attributable to immediate (Gell and Coombs type I) hypersensitivity to human insulin, appeared at the injection sites. There were no other complications with continued insulin therapy, and after about 6 weeks no further local reactions were detectable. When an allergic reaction to an insulin preparation is suspected, careful immunological investigation should be performed, to ensure adequate treatment without risk to the patient.

Delayed-Action Preparations↗
PubMed1991

Phenotyping of immunocompetent cells in normal labial and palatal salivary glands and in non-autoimmune sialadenitis.

Different types of inflammatory cells in healthy major and minor salivary glands (SG), including those in labial and palatal non-autoimmune sialadenitis, were quantified immunohistochemically. Plasma cells, mainly IgA type predominated in all SG types, with the smallest number seen in the palatal glands. The numbers of common leukocyte antigen (CLA) reactive lymphocytes were greater in major SGs than in minor ones and were predominantly UCHL1 positive T cell type. Macrophages and neutrophils were absent in palatal glands, rarely present in labial ones and usually present in major SGs. Increases in the number of IgG and IgM plasma cells and lymphocytes (CLA+) which include both UCHL1+ T and L26+ B cell types, were found in non-autoimmune labial and palatal sialadenitis. There was no significant correlation between the number of the inflammatory cells and the degree of glandular atrophy in both labial and palatal non-autoimmune sialadenitis. Increase in their number represents a protective response of these glands in contrast to the inflammatory cells in major autoimmune sialadenitis playing there a pathogenetic role.

Antigens, Differentiation↗
PubMed1991

[Laugier-Hunziker-Baran syndrome].

Laugier-Hunziger-Baran syndrome is characterized by lenticular melanin pigmentation of the lips and oral mucosa that may be associated with longitudinal melanonychia. Neither a systemic nor a local cause is demonstrable. There is no risk of development of oral or subungual malignant melanoma. Fifteen cases of this syndrome, which has not yet been reported in the German literature, are described.

Adult↗
PubMed1991

Fungal infections of the nail.

Onychomycoses represent the most frequently seen nail diseases and are the most difficult to treat of all skin mycoses. They are rare in children and increase in incidence with age. Most cases are caused by dermatophytes, in particular by Trichophyton rubrum, less frequently by T mentagrophytes and Epidermophyton floccosum. Molds may secondarily infect nails already diseased; however, some are probably capable of primary invasion of nail tissues. Yeasts, particularly Candida albicans, are mainly isolated from fingernails in chronic paronychia and onycholysis, and from nails in chronic mucocutaneous candidosis. Mixed infections by dermatophytes, molds, and/or yeasts are not uncommon. Probably, most fungi cannot infect a healthy nail organ, and only predisposing factors such as impaired blood circulation, peripheral neuropathy, diabetes mellitus, damage from repeated minor trauma, and limited immune defects as well as AIDS make the nail susceptible to fungal infection. Most onychomycoses are secondary to a mycosis of the adjacent skin. Distallateral subungual onychomycosis starts at the hyponychium spreading proximally to the nail bed and matrix. In proximal subungual onychomycosis, the fungus infects the cuticle and eponychium to reach the matrix where it becomes enclosed into the nail plate substance. Total dystrophic onychomycosis may result from either form or develop in chronic mucocutaneous candidosis. Superficial white onychomycosis is commonly a culture of T mentagrophytes on the surface of a toenail. Mycotic paronychia and onycholysis are usually due to C albicans. Clinically, onychomycoses have to be differentiated from noninfectious onychodystrophy, nail psoriasis, lichen planus unguium, and chronic nail eczema. Despite a considerable number of effective antifungal drugs, treatment has remained difficult because the predisposing factors are usually not amendable to therapy.

Antifungal Agents↗
PubMed1990

Mineralization of collagen and elastic fibers in superficial dystrophic cutaneous calcification: an ultrastructural study.

The ultrastructural morphology of localized skin calcifications without associated diseases and with normal serum calcium and phosphate ion values is still unknown. In a case of superficial dystrophic calcinosis cutis (DCC), the role of collagen, elastin and ground substance in the process of calcification and the organization of the apatite crystals could be studied by light and electron microscopy despite technical difficulties in sectioning the hard tissue. Ultrastructural investigation revealed the nucleation of calcification being related to collagen and elastic fibers. No intracellular calcification was found. A flower-like arrangement of pleomorphic crystals was found around single collagen fibrils resembling the calcification of collagen seen in bone tissue. The elastic fibers showed a different pattern of calcification compared with other diseases (e.g. pseudoxanthoma elasticum) with known calcification of the elastic fibers. The process of mineralization was initially linked to the microfibrils of the elastic fiber.

Adult↗
PubMed1990

[Cytologic methods in dermatology].

Cytology is a simple, fast, and inexpensive procedure yielding a high degree of diagnostic accuracy-provided the examiner has a good deal of experience. It has not yet become generally accepted in the diagnosis of skin diseases, since biopsies are very easy to perform, and histopathology is still more reliable than cytology. Modern techniques such as electron microscopy, immunofluorescence, immunocytochemistry with polyclonal and monoclonal antibodies, immunoelectron microscopy, DNA flow cytometry, and in situ hybridization have considerably increased the potentials of cytology and will certainly help to win general recognition in dermatology, as well.

Biomarkers, Tumor↗
PubMed1989

[Surgical treatment of the male genitalia].

Skin diseases of the male genitalia have a serious psychological impact. The clinical diagnosis is often difficult, and histological diagnosis is, therefore, of utmost importance. Nearly all surgical operations can be made under local or regional block anesthesia. Comparatively simple techniques allow the closure of defects even after excision of large lesions, thus resulting in a good functional and cosmetic outcome.

Genital Diseases, Male↗
PubMed1989

A comparative lectin histochemical study of major and minor salivary glands with special reference to the labial glands.

There is debate about the nature of the secretory cells in labial salivary glands. To characterize their basophilic acini the binding patterns of different lectins were examined in these glands (n = 30) and compared with those of major salivary glands (n = 10) and palatal salivary glands (n = 20). Binding in formalin-fixed paraffin-embedded sections was revealed using either anti-lectin antibodies and the peroxidase-antiperoxidase method or biotinylated lectins and the avidin biotin method. Binding of peanut agglutinin was seen in all basophilic acini of labial glands, whereas serous acini of major glands were completely negative. Focal binding of soybean agglutinin was seen in all basophilic acini of labial glands in addition to diffuse binding in mucous acini of all salivary glands, whereas serous acini were mostly negative. Conversely, there was binding of Ulex europaeus agglutinin I in all mucous and serous acini of all glands except for the basophilic acini of labial glands. Bandeiraea simplicifolia agglutinin I and Helix pomatia agglutinin bound to all basophilic and most mucous acini of labial glands, and to most mucous acini of major salivary glands, whereas only a few serous acini of major glands were reactive with both lectins. Pretreatment with neuraminidase yielded binding sites for peanut agglutinin in most of the serous acini of major glands and mucous acini of labial glands, and increased B. simplicifolia agglutinin I and H. pomatia agglutinin binding in the serous acini of major glands. Thus the different lectin binding patterns, particularly the differences in the reaction patterns of peanut, soybean and U. europaeus agglutinin I, showed that the basophilic acini of labial glands reacted more like mucous acini.

Histocytochemistry↗
PubMed1989

Are differences in filaggrin expression suitable for discriminating benign, premalignant and malignant skin lesions? An immunohistochemical study.

The differential diagnosis of benign and malignant skin lesions may pose considerable difficulties. Therefore, 137 formalin-fixed paraffin-embedded biopsies of various benign, premalignant and malignant skin tumours were examined immunohistochemically for the presence of filaggrin using the monoclonal anti-filaggrin antibody AKHl. The proliferating cells of both benign and malignant tumours were negative for filaggrin. Most of the benign tumours exhibited an intense filaggrin-positive granular layer identical to the adjacent healthy skin whereas benign conditions such as irritated seborrhoeic keratoses and clear cell acanthomas were completely devoid of filaggrin. Keratoacanthomas revealed an inconsistent staining pattern for filaggrin. Premalignant and malignant lesions including basal cell carcinomas were either focally positive or completely negative for filaggrin. The absence or presence of filaggrin in these lesions did not correspond to the degree of dysplasia. Moreover, none of these conditions gave a staining pattern for filaggrin to be used for the histological differential diagnosis of these skin lesions.

Diagnosis, Differential↗
PubMed1989

Comparison of cytokeratin, filaggrin and involucrin profiles in oral leukoplakias and squamous carcinomas.

As the distribution pattern of cytokeratin (CK), filaggrin and involucrin has recently been suggested to discriminate between benign and malignant epithelial growths, biopsies of healthy oral mucosa, leukoplakias without and with dysplasia and squamous cell carcinomas were examined immunohistochemically using a panel of 4 monoclonal antibodies (AB) against different cytokeratin polypeptides (34 beta E12, KL1 and Pkk1) and filaggrin as well as a polyclonal AB to involucrin. Major and statistically significant differences were observed in the profiles of CKs (except Pkk1), filaggrin and involucrin between leukoplakias without and with epithelial dysplasia. However, the alteration in the expression of CKs, filaggrin and involucrin proved to be not a constant feature in leukoplakias with dysplasia as a considerable portion (20-25%) of them revealed the profiles of CKs, filaggrin and involucrin similar to those of benign leukoplakias, and vice versa. Immunostaining of these antigens did not define the diagnosis of dysplasia in leukoplakias more precisely than grading in conventional histology can do so far. However, immunohistochemical sensitivity in detecting a broad range of variation in the abnormal maturation patterns of keratinocytes in leukoplakias with dysplasia can be used to divide these lesions into subgroups to elucidate their prognosis in follow-up studies.

Carcinoma, Squamous Cell↗
PubMed1989

Localization of epithelial markers and defence proteins in minor and major salivary glands.

Immunohistochemical demonstration of cytokeratin (CK), tissue polypeptide antigen (TPA), carcinoembryonic antigen (CEA), lactoferrin (Lf), lysozyme (Ly) and secretory component (SC) was performed in major salivary glands (MaSG), labial and palatal salivary glands (LSG, PSG). CK and TPA were demonstrated in ductal cells of all SGs. In addition, binding of anti-CK (Mw-56 Kd) antibody (AB) KL I was seen in all serious acini of MaSG, basophilic acini and demilunes of LSG and in some mucous acini of PSG. Binding of anti-CK (Mw 44-54 Kd) AB-PKK I, another polyclonal anti-CK (Mw 55-67 Kd) AB-PCK and anti-TPA AB was demonstrated in all basophilic acini of LSG, basophilic portions of mucous acini in PSG and not in acini of MaSG. Significant differences were observed in the expression of CEA, Ly, Lf and SC between acini of MaSG and MiSG which probably reflects different functions of these glands.

Antibodies, Monoclonal↗
PubMed1989

Cutaneous lymphoepithelioid lymphoma (Lennert's lymphoma). Combined immunohistological, ultrastructural, and DNA-flow-cytometric analysis.

Lymphoepithelioid lymphoma (LEL) is a peculiar type of low grade malignant T cell lymphoma usually arising in lymph nodes, characterized immunohistologically by predominant T-helper/inducer lymphocytes intermingled with clusters of epithelioid cells. An uncommon case of LEL with cutaneous involvement is reported with additional reference to ultrastructure and DNA-flow-cytometric analysis (DNA-FCM) of the lymphoma. Light microscopy showed subepidermal bandlike infiltrates of lymphocytes and clusters of epithelioid cells extending into the subcutaneous tissue. By immunohistochemistry the presence of a high percentage of T-helper/inducer lymphocytes was confirmed. DNA-FCM demonstrated an aneuploid cell population indicating malignant cells. Our results are in accordance with earlier ones established in lymph nodes.

Aged↗
PubMed1979

[Seasonal burning palate in allergy against grass pollen].

An immediate type I allergy elicited by IgE antibodies against pollen of grasses and cereals was demonstrated as the rare cause of palatinal stomatopyrosis (burning palate). The casual relationship was suggested through history and proven by skin, radioallergosorbent and exposition tests.

Adult↗
PubMed1979

The Papillon-Lefèvre syndrome: keratosis palmoplantaris with periodontopathy. Report of a case and review of the cases in the literature.

The Papillon-Lefévre syndrome (PLS) is an autosomal recessive trait characterized by diffuse transgredient palmar-plantar keratosis (PPK) and premature loss of both the deciduous and permanent teeth. In most cases, the PPK is noted within the first 3 years of life. The periodontal lesions begin shortly after the start of both the primary and the permanent dentitions. The teeth are affected in the order of their eruption, exhibiting inflammation of the periodontal tissue, bleeding of the gums, pocket formation, loosening, and finally spontaneous exfoliation without showing definite signs of root resorption. After an edentulous interval, the same process begins anew shortly after the second dentition. Ectopic intracranial calcifications, mental retardation, and increased susceptibility to infections have often been seen in PLS patients and may thus be regarded as facultative signs.

Child↗