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Biomedical subjects

E Heinrich

Publications and source records attributed to E Heinrich.

At least 19 recordsLinked to original sources
PubMed2003

TGF-beta cytokines increase senescence-associated beta-galactosidase activity in human prostate basal cells by supporting differentiation processes, but not cellular senescence.

The family of transforming growth factors betas (TGF-betas) comprises molecules involved in growth inhibition, stress-induced premature senescence, epithelial mesenchymal transition and differentiation processes. The aim of this study was to clarify the effect of long term exposure of human prostate basal cells to TGF-betas, which are found in high concentrations in prostatic fluid and areas of benign prostatic hyperplasia (BPH). Basal cell cultures established from prostate explants (n=3) were either grown into cellular senescence, or stimulated with TGF-beta1, beta2 and beta3. Similar to cellular senescence, TGF-beta stimulation resulted in an increase of SA-beta galactosidase (SA-beta-gal) activity, flattened and enlarged cell morphology, and down-regulation of the inhibitor of differentiation Id-1. TGF-beta-treated prostate epithelial cells neither showed terminal growth arrest nor induction of important senescence-relevant genes, such as p16(INK4A), IFI-6-16, IGFBP-3 or Dkk-3. Cells stained positive for cytokeratins 8/18, but did not express other lumenal markers, such as prostate-specific antigen and androgen-receptors. TGF-betas increased also the expression of the mesenchymal marker vimentin, indicating that basal epithelial cells underwent differentiation with lumenal and mesenchymal features. In contrast, in vitro-differentiated neuroendocrine-like cells from prostate organoide cultures, expressing chromogranin A and cytokeratin 18, strongly stained positive for SA-beta-gal. Thus, SA-beta-gal activity is not only a marker for senescence, but also for differentiation of human prostate epithelial cells. With regard to the in vivo situation, in addition to cellular senescence, TGF-beta could contribute to the increased number of SA-beta-gal positive epithelial cells in BPH.

Aged↗
PubMed1998

Vascular and neuroendocrine components in altered blood pressure regulation after surgical repair of coarctation of the aorta.

STUDY OBJECTIVE: To investigate potential vascular and neuroendocrine determinants of altered blood pressure (BP) regulation in patients previously operated on for aortic coarctation. DESIGN, SETTING AND PATIENTS: We prospectively re-evaluated 45 patients operated on for aortic coarctation at Strasbourg University Hospital over a 13-year period. Four of these patients were less than 2 years old at the time of the operation and four were older than 20 years. Patient age and time since the operation were on average 21+/-13 years and 8+/-3 years, respectively. Surgery consisted of a resection with end-to-end anastomosis for 18 patients, angioplasty (8), prosthesis (4) or sub-clavian flap (15). RESULTS: Despite repair of the coarctation, about 40% of the patients showed an abnormal BP status at rest. The majority of these patients had uncomplicated borderline hypertension. The orthostasis test as well as the BP circadian rhythm were frequently abnormal. While the ankle/arm systolic pressure index measured at rest was generally within the normal range, diminished carotid-femoral pulse wave velocity was observed. Plasma adrenaline and aldosterone levels were elevated in about 50% of the patients examined. CONCLUSIONS: These new findings suggest that there are 'cause and effect' relationships between aortic structural and functional vascular abnormalities, and augmented plasma adrenaline and aldosterone in some patients after coarctation repair. These phenomena are likely to be involved in altered BP regulation and might result in recurrent hypertension.

Adolescent↗
PubMed1996

Determination of Platinum-Group Elements (PGE) from catalytic converters in soil by means of docimasy and INAA.

The nickelsulfide fire assay (docimasy) for the enrichment of platinum-group elements (PGEs) has been modified for the use with small samples and combined with instrumental neutron-activation analysis (INAA). This procedure has been applied to the determination of PGEs exhausted from catalytic converters and deposited in soil near the Wiesbadener Kreuz (highway A3, Frankfurt-Köln). Our results show a considerable enhancement of the Pt (up to 330 ng/g), Pd (6.6 ng/g) and Rh (7.5 ng/g) contents close to the highway.

Journal Article↗
PubMed1993

Kinematics of the Zielke procedure (VDS) in scoliosis. Intraoperative measurements.

The Zielke correction technique (VDS) has two parts, derotation and ventral convex-side compression. Both were studied by measurements made during and after the operation. Ventral derotation always creates lordosis. Lordosis can be maintained during compression by insertion of intervertebral bone blocks, or kyphosis created by omitting them.

Biomechanical Phenomena↗
PubMed1992

Correction of the sagittal plane in idiopathic scoliosis using the Zielke procedure (VDS).

This paper reports a retrospective review of 91 patients with idiopathic thoracic scoliosis corrected by Zielke VDS instrumentation. The results were analysed to demonstrate the correction in all three planes. Satisfactory correction was obtained, and was particularly good in the sagittal and horizontal planes. There were no disadvantages from the thoracotomy.

Adolescent↗
PubMed1991

Stabilization of the lumbosacral spine in postlaminectomy syndromes. Technique and 2-year results.

A 2-year follow-up study is presented using transpedicular stabilization with USIS (Universal Spinal Instrumentation System) and dorsoventral fusion for failed back syndrome. Eighty-four % excellent and good results at 3 to 6 months have decreased to 56% at 1 to 2 years. Complications are few if the anatomic landmarks are respected. Many psychological and social factors also modify the final outcome of salvage surgical procedures. Only the relief of pain determined our subjective evaluation by the patient himself, without regard to sensory or motor deficiencies.

Adult↗
PubMed1984

Paediatric surgical principles in the management of children with intersex.

Two children, one with dysgenesis of the gonads and chromosomes 46XY and one with true hermaphroditism, are described. The principles of therapy with special reference to the operative correction of the external genitalia are discussed. One child was made a male and the other a female. The diagnostic procedure, heredity, hormonal status, chromosome analysis, histology of the gonads, radiological investigations, and skeletal age will partly determine the future sex, as will the decision of the parents and sociological aspects. The sex must be determined as early as possible. In girls, operative corrections should be performed at the time when their own oestrogen and gestagen production is starting. In boys enlargement of a micropenis can be produced by careful administration of testosterone.

Adrenal Hyperplasia, Congenital↗