[Megacaryocytopoesis and the number of trombocytes after the transplantation of cells of bone marrow in lethally irradiated mice (author's transl)].
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Biomedical subjects
Publications and source records attributed to E Hermanová.
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Suspension of stored and short-term cultured murine bone marrow cells was i. v. administered to lethally irradiated mice. We demonstrated that the cells still after 72 h of storing and 24 h of culture in liquid medium were capable of proliferation and on day 9 following transplantation could produce colony forming units in the spleens of lethally irradiated mice. We established the relative participation of individual types of cells in the recovery of haemopoiesis in the spleens.
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Changes in cells of mouse bone marrow cultured for 6--24 hr were followed both when fresh, and after storage for 3 to 5 days at a temperature of +2 to +4 degrees C. Considerable alterations were observed in the morphology, activity and transformation of these cells both during the storage period and the subsequent culture. Phagocyte ability was also detected in these cultures.
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