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Biomedical subjects

E Holst

Publications and source records attributed to E Holst.

At least 109 records · Page 6Linked to original sources
PubMed1980

Characterization of Clostridium difficile and its differentiation from Clostridium sporogenes by automatic head-space gas chromatography.

Although 47 strains of Clostridium difficile and Clostridium sporogenes were studied by gas chromatography. Acidic and neutral volatile products, formed after 96 h of incubation in glucose-containing peptone yeast-extract medium, were chromatographed. All strains produced appreciable amounts of fatty acids, which were tentatively identified by gas chromatographic retention data. Chromatograms obtained when analysing diethyl ether extracts of culture media of all 47 strains were virtually identical. However, analysis of the broth media by automatic head-space gas chromatography, employing a glass capillary column, gave chromatographic patterns which differentiated the two Clostridium species studied. C. sporogenes was characterized by chromatographic patterns containing a dominant peak of isovaleric acid. Strains of this species produced larger amounts of early eluting compounds and much smaller amounts of butyric and valeric acid than did strains of C. difficile. Automatic head-space gas chromatography provides an efficient means for differentiation of C. difficile and C. sporogenes. This gas chromatographic technique is easier and more rapidly performed than analysis of either extracts of culture media.

Chromatography, Gas↗
PubMed1979

Cervico-facial actinomycosis. A retrospective study.

A retrospective investigation of 25 cases of verified cervico-facial actinomycosis recorded in the period 1971--76 is presented. The results have been compared with the findings from a previous examination carried out by one of the authors during 1955--64. A marked increase of cervico-facial actinomycosis was noted. The clinical picture seems to be changing to a more alarming appearance, in agreement with the classical description of this entity. According to this agreement with the classical description of this entity. According to this a prolongation of the period of treatment was found. Other aspects of the disease are discussed based on the present results. The recommended treatment is still a combination of antibiotic medication and surgical removal of infectious foci.

Actinomycosis, Cervicofacial↗