Biomedical subjects

E I Grodums

Publications and source records attributed to E I Grodums.

13 recordsLinked to original sources

PubMed1981

Experimental studies of acute and recurrent herpes simplex virus infections in the murine heart and dorsal root ganglia.

The multiplication of HSV-1 and HSV-2 strains in the heart and the corresponding dorsal root ganglia (DRG) was examined in experimentally infected mice. Infectious HSV-1 was recovered from the heart between the second and fourth day after inoculation and 3 days later from the DRG. Both the heart and DRG yielded infectious HSV-2 from the fourth to the twenty-first day after inoculation. The HSV-2, but not the HSV-1, induced recrudescent disease in chronically infected mice up to 18 months after inoculation. These affected mice showed signs of disease similar to those observed during the acute phase of the primary infection. During the recrudescent disease, infectious HSV-2 was recovered both from the heart and DRG. The possibility of using this experimental model for investigating long-term human cardiopathies is discussed.

Animals↗

PubMed1977

Ultrastructural changes in the mitochondria of brown adipose cells during the hibernation cycle of Citellus lateralis.

The mitochondrial structure in the brown adipose cells of the golden mantled squirrel, Citellus lateralis, was examined throughout the year in biopsy samples. The mitochondria showed remarkable and apparently reversible changes in size and internal structure related to the physiologic activity of the animal. In the active animal the size of the largest mitochondria was 2.4 micrometer X 1.5 micrometer; during hibernation it increased to 7 micrometer X 2.5 micrometer; and during arousal it reached 11.2 micrometer X 5.3 micrometer. The cristae of the mitochondria in the brown adipose cells of the animals in hibernation phase formed loops, whorls and mesh-like interconnections. During the arousal phase they underwent further configurational changes. The most remarkable structure was associated with mitochondria of most unusual proportions which by dissolution gave rise to a new generation. This was a common finding during arousal but did not occur in any other phase of the hibernation cycle. The new mitochondria were virtually indistinguishable from those of brown adipose cells of any active animal.

Adipose Tissue, Brown↗

PubMed1976

Ultrastructure of mouse periventricular and choroid plexus tissues in experimental vesicular stomatitis virus infection.

The ultrastructure of the periventricular and choroid plexus tissues was studied in the three-week-old mouse, infected with the vesicular stomatitis virus (VSV) by the intranasal route. The VSV reached the periventricular ependymal lining late in infection. It was carried within the cytoplasm of myelinated axons and their sheath cells. Inflammatory cells, although few in numbers, may have also contributed to the virus dissemination. Once in the periventricular tissues the VSV entered the cells from the intercellular space. It replicated in both the ependymal and subependymal cells. It matured either directly in the cytoplasm, or by budding from the plasmalemma as well as the cytoplasmic membranes. In numerous cells the cytoplasmic membranes formed the so-called undulating tubules which contained the VSV particles. The infected ependymal cells became necrotic and separated from the ventricular surface. The changes were similar in all three of the cerebral ventricles. At no time during the infection was the VSV observed near or within any of the choroid plexuses. Some ultrastructural changes, however, occurred in the choroidal epithelial cells. These may have developed either as a consequence of viral damage in the periventricular tissues, or due to virus presence in the cerebral ventricles.

Animals↗

PubMed1976

Experimental herpes simplex virus carditis in mice.

Herpes simplex virus types 1 and 2 induced acute and chronic cardiac damage in suckling and weaning mice after intranasal inoculation. Signs of virus replication were detected by light, immunofluorescent, and electron microscopy techniques. Virtually all of the cardiac tissues appeared to be susceptible to herpes simplex virus. The myocardium, however, was most regularly affected. The viral lesions were discrete during the acute phase of infection. The cardiac damage, however, was more extensive in some of the chronically infected mice. Morphologically, these lesions either resembled the acute ones or were associated with inflammatory granulomatous and sclerotic changes.

Acute Disease↗

PubMed1972