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E J Anstee

Publications and source records attributed to E J Anstee.

11 recordsLinked to original sources
PubMed1998

HIV-1 selection by epidermal dendritic cells during transmission across human skin.

Macrophage tropic HIV-1 is predominant during the initial viremia after person to person transmission of HIV-1 (Zhu, T., H. Mo, N. Wang, D.S. Nam, Y. Cao, R.A. Koup, and D.D. Ho. 1993. Science. 261:1179-1181.), and this selection may occur during virus entry and carriage to the lymphoid tissue. Human skin explants were used to model HIV-1 selection that may occur at the skin or mucosal surface. Macrophage tropic, but not T cell line tropic strains of HIV-1 applied to the abraded epidermis were recovered from the cells emigrating from the skin explants. Dermis and epidermis were separated by dispase digestion after virus exposure to determine the site of viral selection within the skin. Uptake and transmission to T cells of all HIV-1 isolates was found with the dermal emigrant cells, but only macrophage tropic virus was transferred by emigrants from the epidermis exposed to HIV-1, indicating selection only within the epidermis. CD3+, CD4+ T cells were found in both the dermal and epidermal emigrant cells. After cell sorting to exclude contaminating T cells, macrophage tropic HIV-1 was found in both the dermal emigrant dendritic cells and in dendritic cells sorted from the epidermal emigrants. These observations suggest that selective infection of the immature epidermal dendritic cells represents the cellular mechanism that limits the initial viremia to HIV-1 that can use the CCR5 coreceptor.

CD4-Positive T-Lymphocytes↗
PubMed1988

Giant inguinal hernia.

Giant inguinal herniae present a major challenge in management. This case details clinical features of an enormous inguinoscrotal hernia associated with septic gangrene and elephantiasis of the scrotum. Two initial operations were required for control of sepsis, followed by a two-staged hernial repair, involving a total colectomy and a subsequent neoscrotal repair. The problems of loss of domain within the abdominal cavity and the special features presented by this case are discussed.

Colectomy↗
PubMed1986

Post-mastectomy breast reconstruction: the soft tissue alternative.

The available methods of soft tissue reconstruction of the breast are described. They are usually preferable to the use of a prosthesis. For unilateral reconstruction the appropriate method depends on the age of the patient and the size of the breast. In an elderly patient with large breast, a two-stage breast-sharing procedure is advocated. In a young patient with a small breast, an extended latissimus dorsi myocutaneous flap provides a suitable reconstruction. In a middle-aged woman with a breast of moderate size and an adequate amount of abdominal subcutaneous tissue, the most appropriate method of reconstruction is a rectus abdominis myocutaneous flap. For bilateral reconstruction where the consistency is less important, a prosthesis, with or without a latissimus dorsi flap for cover, is the method of first choice.

Abdomen↗
PubMed1984

Soft tissue reconstruction of the breast using an extended composite latissimus dorsi myocutaneous flap.

A method of breast reconstruction using the whole of the latissimus dorsi muscle is described in which the usual skin flap is extended to include a large de-epithelialised flap of dermis and subcutaneous tissue from the loin to provide the necessary augmentation with living tissue rather than with a prosthesis. The method can be used as a reliable one-stage reconstruction, without a prosthesis, either secondarily or as a synchronous combined procedure at the time of the mastectomy.

Adult↗
PubMed1981

Post-mastectomy breast reconstruction using a direct flap from an abdominal lipectomy.

If a patient requires a breast reconstruction and at the same time is a candidate for an abdominal lipectomy, it is possible to combine the operation and carry out a breast reconstruction using a direct flap from the abdominal wall. The technique of this two-stage operation is described. In suitable cases one can produce a breast resembling the normal without any obvious scarring to detract from the result and without the complication of a silastic prosthesis.

Abdominal Muscles↗
PubMed1977

Reconstruction of the breast following radical mastectomy.

A method of reconstruction of the breast following radical mastectomy is presented. The technique involves the use of an individually made Silastic prosthesis inserted three to six months after the mastectomy. A new simple method of nipple reconstruction is described, and the total restoration can be achieved in one operation. It is suggested that reconstruction of the breast be considered more frequently in patients subjected to mastectomy.

Breast↗