Proceedings: Potentiation of picrotoxin-induced convulsions in mice by antidepressants. Specificity of the effect.
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Biomedical subjects
Publications and source records attributed to E J Harry.
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A sample of fixed bacterial cells was examined by immunofluorescence microscopy using an Alexa 488 conjugated secondary antibody for visualization. Excitation using visible light confirmed the expected photostability of this fluorophore; however, when using 2-photon excitation, Alexa 488 was rapidly and substantially photobleached. The unexpected instability of Alexa 488 under certain conditions may have deleterious consequences if not anticipated and accommodated in experimental protocols.