Biomedical subjects
Publications and source records attributed to E J Hicks.
In the present investigation we did not observe age or sex differentials in the prevalence of sickle cell or HbC-traits in Black males or females of low socioeconomic status. When our data were compared to those of others, we found no evidence for a socioeconomic differential in the prevalence of these traits.
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The relative sensitivities of radioimmunoassay, crossoverelectrophoresis, and agar gel immunodiffusion for detecting HBAg in unconcentrated versus concentrated serum samples are compared and discussed. The sensitivities of the various immunologic methods were enhanced severalfold in concentrated serum samples. This is a fundamental consideration in designing a screening program for detecting HBAg and anti-HBAg antibody in hospital patients and donors. The authors propose that serum samples that have marginal reactions or low counts (RIA) be concentrated with Lyphogel or MiniconTM-125 concentrator and retested before test results are reported. Specificity of positive results was studied by neutralization tests with human serum containing antibodies to HBAg.
We compare and discuss three electrophoretic methods for identifying hemoglobins S, A, C, F, and D or G. Electrophoresis on citrate agar gel was more sensitive than electrophoresis on cellulose acetate for detecting hemoglobins S and F, a fundamental consideration in designing cord-blood screening programs for detecting hemoglobin S carriers. Electrophoresis on starch gel is evidently an acceptable method for subtyping hemoglobins AA, CC, AS, SS, AC, and SC, and is more sensitive than cellulose acetate for identifying hemoglobin A1A2. Costs for the citrate agar gel, cellulose acetate, and starch gel procedures are presented.
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Liquid scintillation counting solutions for radioimmunoassay of hepatitis associated antigen (HBsAg) are evaluated and discussed. Data is also presented which indicates that liquid scintillation counting could be carried out by placing cut-off Ausria-125 test tubes in counting vials containing 10 ml of either Brays, Unogel, or Instagel solutions. The data suggest that Brays was the least effective and Unogel and Instagel were the most effective liquid scintillation counting solutions. This is a fundamental consideration if a laboratory is contemplating an initial investiment in counting equipment. The acquisition of a liquid scintillation counter provides additional capabilities for the clinical laboratory interested in radioisotope measurements. In addition, if a laboratory already has a liquid scintillation counter (Beta counter), the director of the clinical laboratory may elect not to purchase additional counting equipment (gamma counter) since he already has the added advantage of measuring iodine-125 with the Beta counter. The authors propose liquid scintillation (RIA) as an alternative counting system for HBsAg detection in hospital patients and donors.