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E J LOWBURY

Publications and source records attributed to E J LOWBURY.

At least 19 recordsLinked to original sources
PubMed1964

A NEW SELECTIVE BLOOD AGAR MEDIUM FOR STREPTOCOCCUS PYOGENES AND OTHER HAEMOLYTIC STREPTOCOCCI.

Horse blood agar containing polymyxin B sulphate, neomycin sulphate, and fusidic acid inhibited the growth of Staph. aureus, Ps. pyocyanea, Proteus mirabilis, E. coli, and Klebsiella pneumoniae but allowed good growth of, and haemolysis by, Str. pyogenes. In a comparison with blood agar, blood 4% agar, and gentian violet blood agar, the selective medium (P.N.F.) yielded a significantly higher proportion of streptococci than the other media, both by aerobic and by anaerobic culture, from burn swab extracts deliberately contaminated with Str. pyogenes; P.N.F. culture was more effective with dilute than with heavy inocula of Str. pyogenes, allowing from three to seven times as many recoveries of Str. pyogenes from swabs contaminated with 10(-3) dilution of streptococcal cultures than cultures of the same material on the other media. Haemolytic streptococci of groups A, C, D, G, and others were isolated by aerobic culture from burns in a consecutive series of 1,277 swabs more often on P.N.F. medium than on blood 4% agar. Viable counts of 12 strains (including 11 different serological types) of Str. pyogenes showed some reduction in the numbers of colonies compared with counts on blood agar, and some strains grew more slowly on P.N.F. medium. These limitations, however, were offset and outweighed by the higher final yield of streptococci on the selective medium.

Agar↗
PubMed1962

A cabinet for the detection of fluorescent bacterial cultures.

A cabinet for detecting the fluorescence of cultures on agar media is described. The use of fluorescence as a criterion for detection of Ps. pyocyanea is compared with that of the oxidase reaction. From a series of patients with burns, all colonies of Gram-negative bacilli which gave a positive oxidase reaction yielded fluorescent subcultures on 0.03% cetrimide agar; all fluorescent cultures on cetrimide agar gave a positive oxidase reaction, but a few mixed cultures which fluoresced on blood agar were not picked for oxidase tests because the colonies of Ps. pyocyanea were overgrown by other bacteria. Out of 1,812 swabs which yielded fluorescent subcultures on 0.03% cetrimide agar, 1,739 (96%) showed strong characteristic fluorescence on the initial blood agar plate culture.

Agar↗
PubMed1961

Skin disinfection.

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Disinfection↗