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Biomedical subjects

E Jabłońska

Publications and source records attributed to E Jabłońska.

At least 19 recordsLinked to original sources
PubMed2006

Pentobarbital in tobacco.

The spectrometric analysis of extracts from tobacco and tobacco smoke revealed the presence of pentobarbital in the analyzed substances. Tobacco samples and tobacco smoke were extracted with chloroform, determinations were performed with the Perkin-Elmer Autosystem XL system, on a Turbo Mass spectrometer. Subject to analysis were 4 cigarette brands manufactured in Poland and raw, unprocessed tobacco. The presence of pentobarbital in the analyzed samples was confirmed by the analysis of the mass spectrum of the substance, as well as by comparison of retention time with standard of pentobarbital. The determined pentobarbital concentrations in tobacco amounted to 3-6 microg/cigarette, and in tobacco smoke they were approximately 45% lower. In case of tobacco extracts it can with high probability be excluded that pentobarbital is synthesized during chromatographical analysis. The presence of pentobarbital in tobacco is thus beyond question.

Gas Chromatography-Mass Spectrometry↗
PubMed2006

Effect of IL-18 on leukocyte expression of iNOS and phospho-IkB in patients with squamous cell carcinoma of the oral cavity.

The inducible synthase of nitric oxide (iNOS) is responsible for the synthesis of nitric oxide (NO) in neutrophils (PMN) and in peripheral blood mononuclear cells (PBMC). This enzyme is controlled by a number of cytokines which accomplish their biological effect via e.g. activation of NF-kB pathway. The aim of the present study was to assess the expression of iNOS and production of NO by PMN and PBMC in patients with oral cavity squamous cell carcinoma (SCC), and to examine the role of the NF-kB pathway in the IL-18-stimulated activation of this enzyme. The production of NO and iNOS expression in PMN were reduced, while iNOS expression in PBMC was increased but NO production by these cells remain unchanged. Patients after treatment showed lower intensity of iNOS expression compared to that observed before treatment. Moreover, both before and after treatment iNOS expression was inversely correlated with phospho-IkB expression in PMN and in PBMC. Significantly higher levels of total NO were observed in the serum of Stage IV patients before and after treatment as compared to the control group. Altered expression of iNOS and NO generation by PMN and PBMC may have an unfavorable effect on the course of antineoplastic response in patients with squamous cell carcinoma of the oral cavity. Intensification of iNOS expression and NO production in Stage IV patients, induced by rhIL-18, suggests its beneficial effect on the activity of leukocytes in patients with squamous cell carcinoma of the oral cavity.

Carcinoma, Squamous Cell↗
PubMed2005

Soluble form of TRAIL, Fas and FasL in the serum of patients with B-CLL.

PURPOSE: Although many studies demonstrated expression of TNF family members in the course of B-CLL, there is a little known about relationships between soluble forms of these proteins. Furthermore, there is no study reported on effects of used therapy on this relation. The present study was designed to asses the relationships between the serum concentrations of sFas, sFasL and sTRAIL in patients with B-CLL regarding their correlation with clinical stage and used therapy. MATERIAL AND METHODS: We studied 40 patients with B-cell chronic lymphocytic leukemia (B-CLL) at diagnosis, before treatment and four weeks after therapy. To measure sFas, sFasL and sTRAIL levels in serum commercially available ELISA kits were used. RESULTS: We found increased concentrations of sFas in sera of all patients with B-CLL before treatment in comparison to the control group. There were no significant differences in concentrations of sFasL and sTRAIL between patients and control group. Increased sFasL concentrations after FC and CC therapy as well as decreased concentrations after 2CdA therapy in comparison to values before treatment were found. The concentrations of sTRAIL after FC and CC therapy were higher than those in patients before treatment. CONCLUSIONS: Results obtained suggest that relationship between sFas, sFasL and sTRAIL in sera of patients with B-CLL before treatment may facilitate the growth B leukemic cells. Changes in these relations after therapy with FC and CC can make a contribution to inhibit B cells growth on the apoptosis way in this patient group.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed2003

IL-15 in the culture supernatants of PMN and PBMC and the serum of patients with Lyme disease.

PURPOSE: The aim of this study was to estimate the production of interleukin-15 (IL-15) by neutrophils (PMNs) and peripheral blood mononuclear cells (PBMC) confronted with the serum levels of IL-15 in patients with Lyme disease. MATERIAL AND METHODS: PMN and PBMC were isolated from heparinized whole blood of patients. The cells were incubated for 18 hs at 37 degrees C in a humidified incubator with 5% CO2. After 18 hs incubation, supernatant was removed and assessed for IL-15 using ELISA kits. RESULTS: The results obtained showed significant increase in the ability of patient's PMNs and peripheral blood mononuclear cells (PBMC) to release IL-15. Although PBMC produced higher concentrations of IL-15 than PMN, the quantitative dominance of PMN in the peripheral blood suggest a significant role for these cells in the defense reactions controlled by this cytokine. Similar changes in the secretion of IL-15 by PMN and PBMC in patient group may be caused by the same regulatory mechanisms which influence the functional abnormalities of the cells examined. CONCLUSIONS: A change in the ability of PMN and PBMC to release IL-15 may have various implications for the cellular and humoral response to the Borrelia burgdorferi (B.b.) infection in patients with Lyme disease.

Adult↗
PubMed2002

Priming effects of GM-CSF, IFN-gamma and TNF-alpha on human neutrophil inflammatory cytokine production.

Identifying and evaluating the priming agents for cytokine release by neutrophils might be helpful in controlling the innate immune response of the host. In the present study we examined the role of granulocyte/macrophage colony-stimulating factor (GM-CSF), interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) as priming agents for interleukin (IL)-1beta, IL-6 and TNF-alpha production by stimulated neutrophils from control subjects and malignant melanoma patients. When the cells from controls and patients were preincubated with primer agents, opsonized zymosan-stimulated inflammatory cytokine production was enhanced. The major neutrophil-priming factor for IL-6 secretion by polymorphonuclear leukocytes (PMNs) in the control and patient groups was TNF-alpha. However, GM-CSF and IFN-gamma are also significant primers. GM-CSF priming was critical for the release of TNF-alpha from PMNs in control and melanoma patients. The ability of GM-CSF, IFN-gamma and TNF-alpha to serve as effective priming agents for inflammatory mediator production by PMNs revealed a new role for these cytokines in the innate immune response of the melanoma-bearing host.

Adult↗
PubMed2001

TNF-alpha, IL-6 and their soluble receptor serum levels and secretion by neutrophils in cancer patients.

Simultaneous evaluation of cytokines and their soluble receptor production and the serum levels can be helpful in understanding the local and systemic immune response of a tumor-bearing host. In the present study we examined serum levels of TNF-alpha, IL-6 and their soluble receptors: sTNFRp55, sTNFRp75 and sIL-6R confronted with their production by the polymorphonuclear neutrophils (PMN) from cancer patients. Examinations were carried out in patients with adenocarcinoma breast cancer and squamous cell carcinoma of the oral cavity and related to the clinical course and to different phases of therapy. Secretion of IL-6, sTNFRp55 and sTNFRp75 by PMN appeared to be dependent on tumor type, clinical progression of disease as well as on therapy, suggesting a significant role of these cells at different phases of the immune response to cancer associated with these mediators. Changes in values of TNF-alpha, IL-6 and their soluble receptors in sera of both cancer groups, dependent on tumor type, clinical progression and cancer therapy, could have a diagnostic and prognostic role in cancer disease.

Adenocarcinoma↗
PubMed2001

TNFRs and IL-6R transmembrane receptors expression and release of their soluble forms by neutrophils and mononuclear cells from cancer patients.

TNF-alpha and IL-6 are multipotential mediators involved in the control of many host's reactions to tumour. Their biological effects are mediated through the membrane-bound receptors (TNFRp55, TNFRp75 and IL-16R respectively) which can exist in soluble forms. In the present study we compared release of soluble sTNFRp55, sTNFRp75 and sIL-6R with expression of their membrane-bound on PMN and PBMC. Cells were isolated from patients with cancer diseases with a different location and histological classification. We have found that alterations of membrane-bound TNFRp75 expression and in the secretion of soluble TNFRp75 form, as opposed to other receptors examined, are characteristic features of neutrophils and mononuclear cells isolated from cancer patients. The similar changes observed in the expression of TNFRp75 by PMN and PBMC appear to confirm a significant role of PMN in the tumour response mediated by TNF-alpha. Furthermore, the altered membrane-bound TNFRp75 expression and sTNFRp75 secretion appear to depend on the tumour type.

Adult↗
PubMed2001

Relationship between TNF-alpha, IL-6 and their soluble receptors in the cultures of human PMN, PBMC and WBC.

Numerous clinical and experimental studies have focused on the measurement of cytokines as well as their regulatory proteins, produced by peripheral blood mononuclear cells (PBMC) or whole blood cells (WBC) as parameters for the immune cellular potential of the host. Much less is known about the contribution of neutrophils or polymorphonuclear leukocytes (PMN) in generating TNF-alpha and IL-6 as well as their soluble receptors. In order to compare the role of PMN to other cells of peripheral blood in the secretion of these mediators we estimated here their release by PMN, PBMC and WBC taken from the same blood samples of healthy donors. A significant similarity in the release of IL-6 by PMN, PBMC and WBC indicate that PMN could be an important source of this cytokine in inflammatory and immune reactions. Although PMN exhibited a lower ability to secretion of sIL-6R, TNF-alpha and sTNFRs, the similar relationships between them in the culture supernatants of PMN, PBMC and WBC indicate on the same ability for contribution in reactions controlled by IL-6 and TNF-alpha. Data presented here in data suggest the possibility of employing PMN to examination the cellular innate response of the host.

Adult↗
PubMed2001

[GM-CSF in the culture supernatants of neutrophils and serum levels in patients with inflammation and squamous cell carcinoma of oral cavity].

The aim of this study was to comparison GM-CSF concentrations in the culture supernatants of neutrophils (unstimulated and LPS-stimulated) and the serum of patients with inflammation and squamous cell carcinoma of oral cavity. PMN from both patient groups exhibited decreased the ability to release of GM-CSF. PMN from patients with inflammation secreted a lower concentrations of GM-CSF than those from cancer patients. PMN from patients with inflammation were more reactivity after LPS-stimulation in comparison with cells from cancer group. GM-CSF serum levels in patients with inflammation and cancer were nonsignificantly higher in comparison with control. There was no correlation between GM-CSF in the culture supernatants of PMN and the serum levels in patient groups. The decreased GM-CSF production may lead to unfavourable effects for immune response of patients with inflammation as well as patients with cancer disease.

Adult↗
PubMed2000

Interleukin 6, tumor necrosis factor alpha and their soluble receptors in the blood serum of patients with denture stomatitis and fungal infection.

Determinations of the blood serum levels of interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha) and their soluble receptors (sIL-6R, sTNFR) in denture stomatitis patients (DS) were performed. Serum levels of interleukins and their soluble receptors were measured using the ELISA method. In all examined patients mycological diagnostics were conducted using API 20C AUX stripe tests and an automatic ATB machine. Results were compared with those of healthy denture wearers (D), and controls (C). In DS patients, yeasts were isolated in 90.9%, in D in 66.7% of cases. The most often isolated species in both groups was Candida albicans. Mean concentrations of IL-6 and TNF-alpha were statistically significantly higher in DS and D groups compared to controls. Mean concentrations of sIL-6R were similar in all groups; however, concentrations of sTNFR in both DS and D groups were significantly lower compared to controls. There were no correlations found between values of IL-6 and TNF-alpha nor between examined interleukins and their soluble receptors.

Adult↗
PubMed2000

[Bactericidal properties of neutrophils from peripheral blood (PMN)of patients with Lyme borreliosis].

In recent years in Poland, the interest has increased in studies about tick borne diseases, mainly Lyme borreliosis. Immune response and genotype of pathogen play an important role in the course of this disease. Phagocytic cells, especially PMN are dominant in defence mechanisms against bacterial infections. The main feature of PMN is their ability to destroy pathogenic microorganisms by phagocytosis. The aim of this study was to estimate the phagocytic activity of PMN connected with intracellular respiratory burst in patients with Lyme borreliosis. The PMN activity tests completed were: phagocytosis, spontaneous and reduced of nitrotetralizate blue test (NBT). Decreased phagocytic activity and oxygen metabolism of PMN from patients with borreliosis in comparison with values of controls were found. Normalization of these parameters after treatment was observed. Changed phagocytic activity connected with intracellular oxygen metabolism during the course of therapy was the main observation. Depression of phagocytic activity of PMN connected with oxygen metabolism can influence defence reactions in patients with Lyme borreliosis. It is suggested that changes observed are acquired and associated with Borrelia burgdorferi presence.

Adult↗
PubMed2000

[Evaluation of FcR and CR receptor expression in granulocytes and immune complex level in patients with tick-borne encephalitis (TBE)].

The aim of this study was to estimate expression of FcR and CR membrane receptors of PMN and circulating immune complexes level in serum from infected patients before and after treatment. Before treatment we observed significant decrease of PMN percentage with FcR and CR receptors in comparison to control. After treatment there was increase of PMN percentage with receptors in comparison to values before treatment but it did not reach values of controls. Immune complexes level before treatment was significant higher than controls before treatment and it decreased after treatment but did not reach control level. Depression of phagocytic activity of PMN may be one of the main reasons for spreading viral infection.

Adult↗
PubMed2000

[Clinical and radiologic evaluation of periodontal changes during treatment for anterior crossbite].

The aim of the study was to evaluate the intensity of changes in periodontium under the influence of occlusal trauma and treatment and to correlate these changes with sex and age of patient. An attempt was made to determine when treatment should be started in order to achieve optimal results. 40 patients (19 girls and 21 boys) with anterior crossbite enrolled. The patients were divided into four groups according to age: 7-9 years (group I); 10-14 years with mixed dentition (group II); 15-19 years with early permanent dentition (group III); 20 years and older (group IV). Malocclusion was diagnosed with the Index of Orthodontic Treatment Needs (IOTN) and radiographs. Periodontal status was evaluated on the basis of pocket depth measured with WHO periodontal probe, presence of pale gingival margin, mobility of teeth according to Lindhe, oral hygiene with the Plaque Index according to Sillness and Löe, and gingival bleeding with a modified method of Caton and Polson. X-ray examination was done on two occasions: before and after treatment. Orthodontic and periodontal examination were done 3 times: before treatment, immediately after treatment and 12 months after removal of active appliance. Acrylic plates were used in 38 patients. Radiographs made before and after orthodontic treatment revealed alveolar bone regeneration in the lower incisors which are antagonists of upper incisors in anterior crossbite, in groups I, II and III. The results show that improvement was easiest to achieve in the youngest group. Transient worsening of the evaluated parameters was due to corrective force of the plate. Improvement in the state of periodontium was observed when the teeth were repositioned correctly. Higher values of the evaluated indices were more often observed in girls than boys. Short treatment times make this method a very attractive alternative.

Adolescent↗
PubMed1998

Changes in soluble IL-6 receptor and IL-6 production by polymorphonuclear cells and whole blood cells of breast cancer patients.

The release of soluble interleukin 6 receptor (IL-6sR) and interleukin 6 (IL-6) was investigated in polymorphonuclear and whole blood cell cultures from untreated patients with breast cancer. IL-6sR and IL-6 were determined in the culture supernatants by a sensitive enzymoimmunological assay. We have shown a decreased ability of unstimulated and stimulated polymorphonuclear cells (PMNs) and whole blood cells (WBC) of cancer patients to release IL-6sR in comparison to a control group. Simultaneously, we have also found an increased capacity of unstimulated cells from the patients to produce of IL-6. The used stimulators-zymosan and lipopolysaccharide (LPS) resulted in increased IL-6sR secretion by PMNs and WBC of patients but did not influence IL-6 production by the same cells. We have also determined the levels of IL-6sR and IL-6 in the sera of cancer patients. The mean concentrations of IL-6sR and IL-6 in the sera of patients were higher than those in the control group. We have not noticed any correlations in the culture supernatant and serum levels of IL-6sR or IL-6 between control and cancer patient groups.

Adult↗
PubMed1998

Soluble TNF receptors release by polymorphonuclear cells and the serum levels in breast cancer patients before and after treatment.

Soluble cytokine receptors by binding to circulating cytokines play an important role in the regulation of immune response of the host. Measurement of their release may be helpful in the evaluation of cytokine-mediated reactions in patients with cancer disease. Soluble tumor necrosis factor receptors (sTNF-Rs) in the culture supernatants of PMNs and in the sera obtained from patients with breast cancer were determined. The examinations were carried out before and after treatment involving surgery and chemotherapy. The highest concentrations of sTNFRp55 and sTNFRp75 were observed in the culture supernatants of PMNs from patients before any treatment, the lower concentrations of them were found in the supernatants of PMNs after treatment. Sera from breast cancer patients presented increased levels of sTNFRp55 and sTNFRp75. Surgery treatment resulted in higher concentrations of sTNFRp55 and sTNFRp75. Decreased sTNF-Rs serum levels were obtained in patients after adjuvant chemotherapy. Different ability of PMNs to the release of TNF-regulatory proteins-sTNFRs appears to confirm PMNs participation in TNF-mediated reactions of the host during malignant process via sTNF-Rs release.

Adult↗
PubMed1998

Serum levels of tumor necrosis factor alpha and production of this cytokine by polymorphonuclear cells in breast cancer patients.

Unstimulated as well as zymosan and LPS-stimulated production of tumor necrosis factor alpha (TNF-alpha) by polymorphonuclear cells (PMNs) was investigated in breast cancer patients. Serum levels of TNF-alpha were also determined. Spontaneous TNF-alpha production was lower, comparable with that found in healthy persons. Zymosan and LPS-stimulated PMNs of cancer patients were found to produce significantly lower amounts of TNF-alpha, as compared with PMNs of healthy control. There was no significant differences in the serum levels of TNF-alpha between breast cancer patients and healthy persons. Because the peripheral blood cells contain more than 50% PMNs, the altered TNF-alpha production by PMNs may have a series of relevant implications during malignant process and seems to be one of the factors helpful in determining the cellular immunology potency of breast cancer patients.

Adult↗
PubMed1998

[Soluble IL-6 receptors].

Both subunits of IL-6 membrane--associated receptor, IL-6R and gp130 can exhibit in a soluble forms--sIL-6R and sgp130 respectively. Soluble IL-6 receptor (sIL-6R) can bind IL-6 and mediate IL-6 function through association with gp130 which is the signal transducing protein. In contrast to other receptors, sIL-6R has a potential to enhance the IL-6 biological function. sIL-6R and sgp130 were found in appreciable amounts in body fluids, although the exact cellular source and function of these proteins are not completely clear.

Animals↗