Biomedical subjects
E K Dunham
Publications and source records attributed to E K Dunham.
Immune ascites in the guinea pig: specificity of cells and antibody in an induced peritoneal exudate.
Strain 2 guinea pigs, immunized with Lys10-Lys(Dnp) in CFA and repeatedly injected intraperitoneally with adjuvant, developed ascites. The fluid was harvested over 8 months in total amounts up to 2 liters per animal and contained substantial amounts of cells and antibody which reacted with the immunizing antigen and related peptides. The antibody was of the IgG and IgA classes and showed restricted heterogeneity. Among synthetic Dnp-oligopeptides, both the cells, studied by antigen-stimulated thymidine incorporation, and the purified antibody, studied by fluorescence quenching, demonstrated the same specificity for the immunizing antigen as has previously been noted in lymph node cells and in serum antibody. The technique offers a means for studying more cells and more antibody than has previously been possible from individual guinea pigs.
Synthesis and immunological properties of the oligolysyl-N-epsilon-dinitrophenyllysine and oligolysylalanylalanylalanyl-N-epsilon-dinitrophenyllysine peptide series.
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Interactions between two antigens governed by the poly-L-lysine gene: failure of one to inhibit competitively an in vitro response to the other.
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Genetic control of the immune response: the effect of non-H-2 linked genes on antibody production.
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Mapping the H-2-linked genes governing, respectively, the immune responses to a glutamic acid-alanine-tyrosine copolymer and to limiting doses of ovalbumin.
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Antigen binding and capping by lymphocytes of genetic nonresponder mice.
Radioautographic study of the binding of GAT-(125)I to spleen cells of genetic responder and nonresponder mice demonstrates that among mice not injected with antigen all strains have approximately the same number of antigen-binding cells; after injection with antigen the number of antigen-binding cells increases in responders but not in nonresponders. Nonresponders are shown to make antibody after injection with GAT complexed with an immunogenic carrier, demonstrating the presence of potentially functional B cells in responders and nonresponders alike. When incubated in the warm, antigen-binding cells of both responders and nonresponders concentrate antigen at one pole of the cell, forming caps.