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Biomedical subjects

E Karbe

Publications and source records attributed to E Karbe.

At least 37 records · Page 2Linked to original sources

[Experimental liver and kidney surgery with CO2, CO, holmium, and neodym lasers. Cutting effect, hemostasis, histopathology, and healing (author's transl)].

Various laser devices (CO2, CO, Nd: YAG, and holmium: YAG lasers) have been used on pig livers and on dog kidneys for comparison with conventional surgical instruments (electroscalpel, cryoscalpel, and scalpel). CO2 and CO lasers caused the least tissue damage, followed by the holmium laser; severe damage was caused by the Nd: YAG laser. The order was reverse for coagulative effect. The conventional reference instruments showed a weaker hemostatic effect. Surfaces cut by laser healed in four to eight weeks without complications. Remnants of charred tissue in various quantities could still be detected after eight weeks in all cases where CO2, CO, and Nd: YAG lasers had been used. This obviously did not affect scar formation.

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Experiments on the Babesia bigemina carrier state in East African buffalo and eland.

Three captive East African buffalo (Syncerus caffer) were injected with 5 x 109 bovine erythrocytes with Babesia bigemina pathogenic for cattle. None of the buffalo showed signs of disease, but Babesia were found in blood smears for four days indicating their multiplication. The carrier state of Babesia bigemina was established in two buffalo for 28 and 147 days respectively by sub-inoculation into splenectomized calves, while no parasites were demonstrated 28 days after injection in the third buffalo. Two buffalo shot in Kenya did not appear to be carriers of Babesia gibemina, as shown by sub-inoculation of blood in splenectomized calves. Two captive eland (Taurotragus oryx) were inoculated and their blood investigated as with the experimental buffalo. Neither eland appeared to harbour Babesia bigemina 28 days after injection, even though one was splenectomized. The experimental data indicate that the East African buffalo can be a carrier of Babesia bigemina for at least 5 months, while this parasite did not survive in eland. Buffalo may play a role in the epidemiology of babesiosis in cattle, while eland most likely do not.

Animals↗

[Experimental bone replacement with resorbable calcium phosphate ceramic (author's transl)].

Compact cylindrical implants (5 x 15.5 mm) of seven calcium phosphate ceramics of different formulations that had been implanted for 6 weeks without fixation in the tibia of dogs were examined histologically. The tissue compatibility of the implant turned out to be dependent on the mineralogical and chemical composition of the material: the ceramic material was biocompatible at a CaO/P2O5 ratio between 2:1 and 4:1, the optimum ratio being about 3:1 (tricalcium phosphate). In addition, cylindrical tetracalcium phosphate implatns with an entirely porous structure were implanted in the same manner. After 6 months, they were resorbed to a minor extent. Implants of different calcium phosphate mixtures, on the other hand, were resorbed to a large extend after the same time. The ceramic material was replaced by mineralised bone tissue which had formed directly on the ceramic implant as well as within the pores. There were no foreign body reactions. Investigations with segment-shaped implants which were implanted in the tibia of dogs and fixed with AO-plates or splints extra-cutaneously for about 8-10 weeks, provided information abouth the maximum stress that can be borne by the implants. While implants with a porosity of 75 percent did not sustain the stress after the fixation had been removed, those with a porosity of 45 percent could be subjected to physiological stress after removal of the splints.

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