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E Keiditsch

Publications and source records attributed to E Keiditsch.

At least 55 records · Page 3Linked to original sources
PubMed1979

Interferometric laser holography for determination of the localization and extent of bladder tumor.

In male giant rabbits (n = 10) with and without bladder carcinomas, holographic interferometric investigations with a double impulse rubin laser were made for testing the sensitivity of this method in detection of elasticity changes of the bladder wall caused by tumors. It could be shown that the holographic interferometry enables a distinction to be made between healthy and tumorous regions of the bladder wall by the various interference patterns.

Animals↗
PubMed1977

[Clinical observations to characterize splenomegalic immunocytomas (author's transl)].

In ten patients with predominant splenomegaly the clinical development and progress of disease following the course of some years are described. The hematological values showed a moderate relative lymphocytosis with a slight but inconstant increase in white blood cell counts. A more or less small number of a morphologically distinctive population of plasmacytoid lymphocytes was an important diagnostic criterion and a decrease of at least one class of immunoglobulins could be shown. Only one patient presented a paraproteinemia (IgM). In the serum of six patients atypical unspecific antibodies were found. The histologic and cytologic feature of the spleens which were removed therapeutically was characterized by a mixed infiltration of lymphocytes and plasmocytoid or plasmacellular elements. By this pattern it was possible to classify these cases as lymphoplasmocytoid (resp. lymphoplasmocytic) immunocytomas according to the new Kiel-lymphoma-classification. From the clinical point of view they represented a subunit with markedly prevalent splenic proliferation. Liver and/or bone-marrow however showed in all cases primarily circumscribed infiltrations of pathognomonic cells or did develop them during the course of disease, even after splenectomy. According to the presented observations this disease is to be placed between chronic lymphocytic leukemia and M. Waldenström not only by pathological but also by clinical criteria.

Adult↗
PubMed1977

[Experimental induction of maligant tumors in the urinary bladder of the rabbit].

Suspended material of Brown-Pearce carcinoma is qualified for producing bladder tumors in rabbits after injection into the wall of the exposed, but not opened bladder. The advantages are the rapid growing and the possibility of controlling by endoscopy and radiography. At present we use this tumor-pattern for examination of the effect of different laser beams in experimental bladder tumors.

Animals↗
PubMed1977

[Endoscopic laser therapy of bladder tumors in man].

15 patients with papillary bladder tumors were radiated with a Neodym-YAG laser beam since summer 1976. The seize of the tumors ranged from 5 to 10 mm in diameter. Neither complications nor tumor recurrency in the former tumor area could be observed. A final statement about the value of laser radiation in malignant tumors is not yet possible because of the small number of patients and the short control period. However, our preliminary experiences might be a beginning of a successful improvement of bladder tumor therapy by use of endoscopic laser radiation.

Argon↗
PubMed1977

Lymphangioma of the duodenum. (Diagnosis by endoscopic resection).

Submucosal lymphangiomas of the gastrointestinal tract are extremely rare entities. A case of a lymphangioma of the duodenum in a 66-year-old woman is reported. Diagnosis was made by endoscopy and tumor excision with a diathermy snare. The patient complained of crampy upper abdominal pain which only partially was referable to an gastric ulcer and stopped after polypectomy. Endoscopically, compressibility of the tumor is a characteristic sign. Roentgenographically, it may be easily overlooked.

Aged↗
PubMed1976

[Morpholgical changes in the rabbit's bladder after argon laser irradiation].

After developing a flexible fiber light guide and a special cystoscope it was possible to study the Argon laser beam effects on the bladder wall of rabbits and dogs. It could be proved that the Argon laser is qualified for the use in organs with thin walls, especially in the urinary bladder, because of its strong absorption in tissue with high blood flow and its minor penetretion depth. However, our further investigations showed--they shall be published--that the output of the Argon laser used in our studies not sufficient for destroying human bladder tumors. For this reason it is necessary to use neodymium YAG laser.

Animals↗
PubMed1976

Endoscopy in experimental urology using an argon-laser beam.

For the first time cystoscopic argon and neodymium-YAG laser irradiation of the bladder wall using a flexible quartz fiber light guide (as described by Nath) was studied morphologically and histologically. It was seen that it is possible to control the depth of tissue removal without the risk of perforation of the bladder wall. Therefore endoscopic surgery of bladder tumors by laser radiation seems to be possible. Hydronephrosis could not be observed even after direct laser irradiation of the orifices.

Animals↗
PubMedDate not supplied

Radioimmunodetection of human glioma xenografts by radiolabelled monoclonal antibodies.

Radiolabelled monoclonal antibodies (131I-MUC 8-22, 131I-MUC 2-63) were used for external scintigraphy of human glioma xenografts. To induce transplantation tumors. 5 x 10(6) cells (85HG-66) of an in vitro established human malignant astrocytoma (N66/85) were inoculated s.c. in BALB/c-nu/nu mice. The labelling of the immunoglobulins with 131iodine was carried out according to the iodogen method, the nude mice, bearing xenograft, received 30 m. 131I-labelled intact monoclonal immunoglobulins (200mCi: 7,4MBq) and the imaging was performed on days 4, 8 and 12 after the application. After 4 days, a clear tumor accumulation of iodinated MUC 2-63 antibodies recognizing surface determinants was visible. This enrichment of monoclonal antibodies (MAbs) led to a characteristic tumor presentation on day 8. Obviously, the MUC 2-63 antibodies remain in the tumor tissue for a long time, so that even on day 12 satisfactory tumor imaging is possible. On the other hand, neither with normal mouse IgG nor with MUC 8-22 antibodies - which react with intracellular structures - could a tumor localization be achieved. The result of the studies on the distribution of 131I-MUC 2-63 on day 19 was that the activity in the tumor tissue was about 4.4 times higher than in the blood and even more times higher than in solid organs.

Animals↗
PubMedDate not supplied

Antigen variation in a human glioblastoma: from the primary tumor to the second recurrence, permanent cell line and xenotransplantation tumors.

Antigen expression in a human glioblastoma was investigated by immunochemical methods in the primary tumor, the first and second recurrence, a permanent cell line derived from the first recurrence and in its xenotransplantation tumors. In the primary tumor, GFAP, vimentin, S100, Leu-7 and glioma-associated antigens (GAA) as defined by the monoclonal antibodies (mAbs) MUC 2-39, MUC 8-22 and MUC 2-63 were markedly expressed. In the recurrences, gradual loss of GFAP and Leu-7 could be observed, whereas S100, vimentin and GAA gave similar results to those in the primary tumor. In contrast, fibronectin and collagen IV, which were restricted to the vessel walls in the primary tumor, were represented in sarcomatous areas of the recurrences. In some of these areas, co-expression of glial cell markers was observed. In short-term cell cultures, expression of glia- and glioma-associated antigens as well as fibronectin and collagen IV was comparable to that of the recurrent tumor tissue. In long-term passages, immunoreactivity of GFAP, Leu-7 and S100 decreased, whereas GAA, vimentin and fibronectin increased. Collagen IV positive cells were not visible beyond passage 15. Transplantation tumors were only partly positive for glial cell markers, but revealed strong immunoreactivity for GAA, fibronectin and collagen IV. With these observations we confirm that the phenotypic variability of glioma cells makes it difficult to identify the origin of cells in human glioblastomas from their antigenicity.

Animals↗
PubMedDate not supplied

Antigenic heterogeneity of human brain tumors defined by monoclonal antibodies.

The antigenic profiles of human gliomas and in vitro established cell lines were investigated using the monoclonal antibodies (MABs) MUC 8-22 and MUC 2-63. The reactivity with tissue samples and cytospin preparations obtained from 45 brain tumors was estimated by the indirect immunoperoxidase technique. In addition, computer-assisted cytofluorometry was used to quantify the intensity and distribution of antibody-binding. Various degrees of antibody-binding among and within gliomas and glioma-derived cell lines were observed. The data show that a variable percentage of cells are not labeled with the employed MABs. The spectrum of reactivity of the selected antibodies was independent of the histological grading of gliomas. However, there were significant differences in various stages of subcultivation of glioma lines. In most cases, the heterogeneity of antigen expression decreased during successive in vitro propagation of glioma cells. The extent of variation in staining intensity values differed within cell populations and reflected the antigenic heterogeneity of human brain tumors. The findings presented here suggest that the use of MABs which recognize glioma-associated antigens facilitates the objective analysis of brain tumors and is of potential value for immunohistochemical application in surgical neuropathology.

Antibodies, Monoclonal↗