Biomedical subjects

E Klingler

Publications and source records attributed to E Klingler.

11 recordsLinked to original sources

PubMed1999

Automated computer-assisted evaluation of diagnosis-and-procedure-reports in Austrian hospitals.

Austria's 320 hospitals with 73,000 beds have to transmit a minimum basic data set for each hospital stay with diagnosis and procedures as well as scores for use in intensive care units (last only in performance-oriented financed hospitals). Based on these data, 160 hospitals or 53,000 beds are financed through performance oriented flat rates per case (LDFs--Leistungsorientierte Diagnosen-Fallpauschalen). Those LDFs are in some principles similar to DRG's (diagnosis related groups), but have been developed by the Federal Ministry of Labor, Health and Social Affairs (BMAGS). 1.94 million data sets of hospital stays, which were reported for financing in the year 1997 had to be analyzed for their correctness and plausibility. For this aim the "Scoringprogramm" of the BMAGS is in use. We describe in detail how many diagnoses and procedures are automatically checked with diagnosis, sex, age, length of stay etc. 1.6% warnings resulted from this checking, and no errors have been reported. The checking routines have to be further developed. At present data seem to be used only with caution for epidemiology or for analyzing medical quality. For financing purposes it might be reliable, especially if data quality commissions are enlarged and if evaluation of data with medical records is improved.

Austria↗

PubMed1984

Circadian rhythm of mechanically mediated differentiation of osteoblasts.

Rats entrained to alternating 12 h light/dark periods were sacrificed at hourly intervals over one complete circadian cycle. Each animal was injected with 3H-Thymidine 1 h before death. Autoradiographs of serial sections of maxillary first molar periodontal ligament (PDL) were prepared. Nuclear volume was determined for labeled fibroblastlike PDL cells along a physiological bone forming surface. Preosteoblasts (large nuclei), the immediate proliferating precursors of osteoblasts, were found to synthesize DNA primarily during the environmental light period and divide during the subsequent dark cycle. Less differentiated precursor cells (small nuclei), the proliferating predecessors of preosteoblasts, were in S phase primarily during the dark period and divided in the following light cycle. Since previous studies have indicated, the stress/strain-mediated increase in nuclear size to form preosteoblasts also requires about 8-12 h, the least complex osteoblast differentiation model, which is consistent with the present data, is a 60 h sequence involving at least four cell types and five alternating dark/light cycles. The principal rate-limiting step in osteoblast differentiation is the mechanically related shift in nuclear size (change in genomic expression) associated with formation of preosteoblasts.

Animals↗

PubMed1982

Nuclear size as a cell-kinetic marker for osteoblast differentiation.

A nuclear morphometric assay for preosteoblasts is introduced as a cell-kinetic technique, applicable to routine histological preparations of mineralized tissue. Because this method is a morphological marker for osteoblast precursor cell differentiation, it provides a new dimension for determining the mechanism of osteoblast histogenesis. Osteoblast precursors of the periodontal ligament are a mixed population of progenitors, kinetically separable into two distinct groups according to nuclear size. Preosteoblasts, the immediate proliferating precursors of osteoblasts, have large nuclei (greater than 170 micrometers3) and are derived from relatively undifferentiated fibroblastlike cells, which have smaller nuclei (less than 80 micrometers3). Increase in nuclear volume, during G1 phase of the cell cycle, is apparently a morphological manifestation of change in genomic expression. This key event in preosteoblast differentiation is related to mechanical stress/strain and may be an important rate-limiting step in osteoblast histogenesis.

Animals↗

PubMed1982