The solution of pharmacological problems with computers. Part 9: Kinetics of the inhibition of the enzyme morphine-N-demethylase by N-allylnormorphine. Mathematical analysis by a FORTRAN-program.
In this paper, we present a method for statistical analysis of enzyme-kinetic data. This analysis allows determination of the character of the inhibition of an enzyme reaction, and computes the 95% confidence limits of the dissociation constants and of Vmax. This method involves the solution of the Michaelis-Menten equation, (see article for formula), using the Guass-Newton iteration.
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