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Biomedical subjects

E Krauss

Publications and source records attributed to E Krauss.

10 recordsLinked to original sources
PubMed1990

Serologic tests for Lyme disease. Interlaboratory variability.

The serologic test for the detection of antibodies to Borrelia burgdorferi is the most frequently used laboratory method for the diagnosis of Lyme disease. However, the insensitivity of the assays and the interlaboratory variability are frequent problems. To determine the extent of this variability, one aliquot of serum from each of nine patients with a history of Lyme disease was sent to nine reference laboratories, including national, university, state, and local hospital laboratories. A second aliquot of the original serum was submitted 2 weeks later. Wide variability among laboratories was observed, ranging from a university laboratory that detected antibody to B burgdorferi (IgG or IgM) in 18 of 18 specimens, to a state laboratory that detected antibody in only 8 of 18 specimens. Detection of IgM specific antibodies showed similar variability (range, 2 to 10 of 18). There were eight instances of a fourfold or greater change in titer between the aliquots sent 2 weeks apart, although only three of these were an increase in titer. These results indicate the need for standardization of the assays and the availability of national reference material. It is recommended that the results of serologic testing should not be relied on as the sole criteria in making the diagnosis of Lyme disease.

Antibodies, Bacterial↗
PubMed1987

Bioavailability of zinc from zinc-histidine complexes. II. Studies on patients with liver cirrhosis and the influence of the time of application.

Supplementation may benefit patients with liver cirrhosis. Zinc uptake from zinc-histidine complex 1:2 was assessed in eight patients with liver cirrhosis. Influence of the time of application was also studied. Compared with healthy controls, patients showed a 40% lower uptake of zinc after 20 mg zinc from zinc histidine. Bioavailability was identical when zinc was taken 6 h or 1 h before a meal but an order of magnitude greater than with or 1 h after a meal. No significant increase of serum zinc was found when zinc was given with a meal or 1 h after. Lower doses of zinc-histidine complexes than of zinc sulfate may be used to supplement patients with liver cirrhosis. Time of application is of great importance if this substitution is to be successful.

Adult↗
PubMed1986

Interaction between human serum albumin and alpha 1-acid glycoprotein in the binding of lidocaine to purified protein fractions and sera.

The authors have determined the binding parameters for [14C] lidocaine to several preparations of purified human serum alpha 1-acid glycoprotein (AAG) and human serum albumin (HSA). The authors find that the average Kd for the three different purified AAG preparations was 15.2 +/- 0.5 microM, with an average of 0.567 +/- 0.009 binding sites (N) per AAG molecule, when the concentration of AAG was determined by a standard colorimetric protein assay. The number of binding sites per AAG increased to 0.99 +/- 0.06 when the concentration of AAG was determined immunologically. On the other hand, delipidized AAG had a Kd of 28.0 microM, with an N of 1.72 based on the immunological assay. Various preparations of HSA had Kd/N values ranging from 4.3 to 17.3 mM. The number of binding sites per HSA molecule could not be determined because the maximum concentration of lidocaine that can be used is 10 mM, which is only approximately equal to the Kd. The Kd to HSA was so low and the capacity so high that, at therapeutic concentrations, the binding was in the linear range. Solutions containing both purified AAG and HSA had Kd values of 30.2 +/- 1.8 microM, with 2.01 +/- 0.04 binding sites per AAG molecule. With HSA and delipidized AAG, the Kd was 60 microM, with 3.16 sites per delipidized AAG molecule. This increase in the binding parameters appeared to be due to a direct interaction between the two proteins.(ABSTRACT TRUNCATED AT 250 WORDS)

Binding Sites↗
PubMed1980

Oxalate-induced acute renal failure in Crohn's disease.

In a patient with long-standing Crohn's disease and bowel resection, acute reversible renal failure associated with hyperoxaluria developed. The renal biopsy specimen demonstrated marked oxalosis. Acute renal failure, although rare, is a complication of enteric hyperoxaluria of Crohn's disease. This type of renal failure may be reversible if recognized and treated early, since remission of symptoms occurred following hemodialysis.

Acute Kidney Injury↗
PubMedDate not supplied

Aseptic necrosis of the femoral head associated with pregnancy; a case report.

A documented case of beginning aseptic necrosis of the femoral head associated with pregnancy together with a review of the literature about this rare complication of pregnancy is presented. The patient complained of increasing pain in the right hip and limitation of movement of the affected joint. After delivery, the diagnosis of beginning avascular necrosis of the right femoral head was confirmed on X-ray, technetium-99 bone scan and CT-scan of the hips. Three weeks after delivery the patient was treated with drilling of the femoral head. Recovery was complete.

Adult↗