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Biomedical subjects

E Kuhlmann

Publications and source records attributed to E Kuhlmann.

14 recordsLinked to original sources
PubMed1994

Mutated c-K-ras in small pancreatic adenocarcinomas.

The objective of this study was to determine whether small human pancreatic adenocarcinomas contain activated c-K-ras as an approach to answering the question of whether c-K-ras mutation is an early change in this disease. Eight pancreatic adenocarcinomas in the range 1.2-3 cm were analyzed for c-K-ras mutation at codon 12 by amplifying the c-K-ras gene around codon 12 out of paraffin-embedded tissue sections using the polymerase chain reaction. c-K-ras mutations were detected by allele-specific oligonucleotide hybridization. Six of the eight small pancreatic adenocarcinomas contained mutated c-K-ras at codon 12, position 2, and two of the six tumors had an additional mutation at position 1 of codon 12. Our results indicate that small pancreatic adenocarcinomas are similar to large, late-stage pancreatic adenocarcinomas in that 75% of the tumors analyzed contain mutated c-K-ras at codon 12, position 2. These data suggest that c-K-ras mutation occurs early and may therefore have a role in initiation of human pancreatic adenocarcinoma.

Adenocarcinoma↗
PubMed1993

Evaluation of c-K-ras in pancreatic carcinomas from Ela-1, SV40E transgenic mice.

c-K-ras is activated by mutation at codon 12 in the majority of human pancreatic carcinomas of ductal, but not acinar, phenotype. Therefore, evaluation of c-K-ras in experimentally induced pancreatic carcinomas in animal models is of interest for comparison with the human disease. Acinar cell carcinomas and islet cell tumors arising in two strains of transgenic mice carrying the Ela-1-SV40E transgene were evaluated using the polymerase chain reaction and allele specific oligomer hybridization to seek evidence of c-K-ras mutation at codons 12, 13 and 61. Amplified DNA products, including codons 12 and 13, from the majority of these tumors were also evaluated by single strand conformation polymorphism analysis. Only wild type c-K-ras was found in the tumors.

Animals↗
PubMed1990

[Mapping the spontaneous EEG in focal disorders].

It will be reported about 142 EEG spectral analytic mappings in 100 patients with focal EEG findings which were recorded already in routine EEG examinations. Drawings of the visually analysed paper recordings have been compared with the maps. We found in about 30 per cent a true gain of information. It is based on a more precise demarcation of focal findings or a better display of points of most severe dysfunction within extended disturbances. Beyond that the pictorial presentation of topographic features is a general advantage of EEG mapping. On the other side there are limits and disadvantages of topographic EEG analysis. With special reference to the analysis of circumscribed dysfunctions they will be demonstrated. Problems are related to the type of reference, to the choice of analysis time, or to "spatial aliasing effects" which are introduced by the interpolation procedure of mapping analysis. Beside the neuroradiological imaging methods the EEG mapping maintains special significance because it displays the functional aspect of cerebral processes.

Brain Diseases↗
PubMed1988

[Lipoprotein metabolism in patients with disordered thyroid gland function before and following therapy].

Clinical manifest hypothyreosis leads to changes of plasma lipoproteins, which are characterized by elevated LDL levels, an increase of the relation LDL cholesterol/HDL cholesterol, an elevation of the quotient cholesterol/triglycerides within the VLDL and an increase of the relation HDL2/HDL3. Both these changes and the diminished activities of post-heparin lipase of hepatic and extrahepatic origin are reversible after substitution therapy. On the contrary, patients with hyperthyreosis M. Basedow show low lipoprotein levels, high activities of post-heparin lipase and an elevation of the relation of the apolipoproteins apo C II/apo C III within the VLDL fraction.

Adult↗
PubMed1987

[Clinical relevance of the determination of TSH-binding inhibitory immunoglobulins (TBII) in patients with immunogenic hyperthyroidism in methimazole therapy].

In 38 patients with immunogenic hyperthyroidism a follow-up was performed to estimate the value of TBII before, during and after methimazole therapy. Before therapy increased TBII were detectable in 37 patients (94.4%). After 12 months methimazole therapy 25 patients had TSH-receptor antibodies (66%) within the normal range. In 13 patients positive antibody titres were found. In most cases persistence of increased TBII-values during drug treatment was an indicator of the persistence of active hyperthyroidism (10 of 13 patients). In the rule a disappearance of TBII-activity was combined with a functional remission (22 of 25 patients). Prolonged demonstration of TBII-activity in conjunction with persistence of hyperthyroidism should lead to ablative measures. In contrast to this medical therapy should be finished in patients with immunological and functional remission. Though in the further follow-up a recurrence of the immunological base of the disease with a functional and clinical relapse is possible.

Autoantibodies↗
PubMed1984

[The thyrotropic function of the hypophysis and peripheral thyroid hormones after removal of bland and autonomous nodular goiters].

Bilateral subtotal resection or enucleation or unilateral resection was performed in 60 patients (36 with bland nodular goitre, 24 with autonomic adenoma). The thyrotropic pituitary activity and the peripheral thyroid function were examined in all patients preoperatively and followed up for 12 months postoperatively. The results show that all patients with bilaterally resected bland nodular goitre require postoperative administration of thyroid hormone, since enhanced thyrotropic activity is seen not later than three months after surgery. Since thyrotropic function remains normal in unilaterally operated nodular goitre, thyroid hormone administration does not appear generally necessary. In patients with autonomic adenoma there is postoperatively variability of function, independent of the surgical method employed. In view of possible functional recompensation, final decision on the administration of thyroid hormones should not be made before the end of the sixth postoperative month. These results can be considered as guidelines for a differentiated postoperative management.

Goiter, Nodular↗
PubMed1984

[Operative findings and therapeutic results in patients with primary hyperparathyroidism].

In the period from 1976 to September 1982 39 patients with the clinical diagnosis of primary hyperparathyroidism were operated on. Among then were 24 women and 14 men. The most frequent manifestation form is the renal one (n = 26). The proportion of patients with hypercalcaemic crisis was very large (n = 4). Topographically and anatomically the adenomas were most frequently found in the region of the right lower pole of the thyroid gland. 23 patients had a singular adenoma, 11 patients showed multiple adenomas and only 2 had a diffuse hyperplasia. In patients with persisting hyperparathyroidism before a repeated intervention an attempt of localization should be performed by a selective phlebography with taking of blood samples for the determination of PTH. The success of the operation is to be read off most clearly at the behaviour of the post-operative values of serum calcium. In 29 patients a normocalcaemia was achieved. 6 patients showed at first a hypocalcaemia, which however, was only temporary in 4 patients. 3 patients had a persisting hyperparathyroidism. In accordance with the literary data the formation of renal calculi clearly decreased after a successful operation.

Adenoma↗
PubMed1979

Serum creatine kinase BB linked to immunoglobulin G.

We describe an atypical form of creatine kinase in the serum of a woman after myocardial infarction. Electrophoresis on agarose gel showed a single fraction between CK-MM and CK-MB isoenzymes. After ion-exchange chromatography on DEAE-Sephadex A-50 we isolated the atypical CK and CK-BB as detected by electrophoresis of the eluted fractions. Results of our immunological investigations and exclusion chromatography clearly demonstrate that the atypical CK consists of complexes with high molecular masses formed by CK-BB isoenzyme and immunoglobulin G. The clinical significance, if any, of this Macro CK has yet to be determined.

Brain↗
PubMed1979

Transplantation of azaserine-induced carcinomas of pancreas in rats.

Two pancreatic adenocarcinomas which had been induced in Wistar/Lewis rats by azaserine treatment were transplanted into rats of the same strain by subcutaneous and intraperitoneal injection of minced tumor. Subsequently, we have serially transplanted into non-radiated recipients. Transplanted tumors have maintained evidence of acinar cell differentiation including the presence of zymogen granules in tumors studied by electron microscopy, and of lipase, amylase and trypsin activity in the supernatant of tumor homogenates. Histologically, the tumors vary from poorly differentiated solid carcinomas to well differentiated variants which form acini. Transplanted tumors are locally invasive and have metastasized to lung and liver in some recipients.

Adenocarcinoma↗