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Biomedical subjects

E Kurstak

Publications and source records attributed to E Kurstak.

13 recordsLinked to original sources
PubMed1979

Studies on the structure of the two infectious types of densonucleosis virus.

No electrophoretic differences were detected between the nucleic acids of the two types of densonucleosis virus, DNV-I and DNV-II. Both virus types had similar isoelectric points. Although the viral proteins found in each type were identical, their stoichiometry differed. The localization of the structural protein, by labeling with periodate-oxidized glycoprotein, supported the hypothesis that 60 molecules of p49 aggregated into a dodecahedron (12 pentamers), whereas the two other proteins (p59 and p69) might have a stabilizing function and were localized on the outer surface of the p49 dodecahedron.

DNA, Viral↗
PubMed1978

Detection of Epstein-Barr virus antigens and antibodies by peroxidase-labeled specific immunoglobulins.

Detection of the Epstein-Barr (EBV) antigens, early antigen (EA), viral capsid antigen (VCA), and nuclear antigen (EBNA) by the indirect immunoperoxidase technique was highly sensitive. Antibody titers to EBNA, EA, and VCA were determined in more than 25 sera of patients with Burkitt lymphoma (BL), nasopharyngeal carcinoma (NPC), or normal persons. A good correlation between the titers of these antigens was obtained by the immunoperoxidase and immunofluorescence methods. The indirect (anti-IgG) immunoperoxidase technique for the detection of EBNA is, in contrast to the indirect immunofluorescence method, highly sensitive. EBNA was associated with the chromosomes in cells arrested in the metaphase with colchicine.

Antibodies, Viral↗
PubMed1978

Morphogenesis of the nodamura virus in the larbae of the lepidopteran Galleria mellonella (L.).

The pathogenesis and morphogenesis of the Nodamura virus, an insect picorna- virus which can also infect vertebrates such as newborn mice, are described in the larvae of G. mellonella. Examination of thin sections of muscle, salivary and moulting glands, hemocytes adipose tissue and hypodermis of the infected larvae of G. mellonella shows in the cytoplasm the accumulation of viral particles either dispersed or in a stringlike or paracrystalline array. These arrays of virions can be within membrane- bound vesicles. Helical filaments of 110 A of diameter can be observed inside the basal membranes and later in the cytoplasm, in relation to the infection of muscular or adipose tissues. The morphogenesis of Nodamura virus in G. mellonella or in suckling mice is very similar to that of the Coxsackie A virus observed in the mouse.

Animals↗
PubMed1977

Biochemical, biophysical, and biological properties of densonucleosis virus (paravovirus). II. Two types of infectious virions.

Among the infectious densonucleosis virus (DNV) particles, two types of virions, DNV-I and DNV-II, have been identified. The density of DNV-I was about 1.40 g/ml and depended on the pH, whereas the density of DNV-II was independent of the pH and was 1.44 g/ml. The sedimentation rates of these particles were 111S and 89S, respectively. The specific extinction coefficients and the absorption spectra, corrected for light scattering, were also different for these two types. The electrophoretic mobility of both types of virions was identical under the experimental conditions used. Both types of particles contained single-stranded DNA with similar physicochemical properties. The difference between these types of virions seemed to be due to a different protein content, DNV-II containing about 600,000 daltons less protein, and to a different quaternary structure as indicated by the change in the density of DNV-II, in the presence of divalent cations, without any change in the density of DNV-I.

Centrifugation, Density Gradient↗
PubMed1976

Biochemical, biophysical, and biological properties of densonucleosis virus. I. Structural proteins.

The polypeptide composition of highly purified densonucleosis virus was studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The viral proteins showed a different behavior in sodium dodecyl sulfate-gels in comparison with the marker proteins. Therefore, the molecular weights were estimated by analyzing the retardation of the electrophoretic mobility of these proteins in gels with increasing polyacrylamide concentrations. Four structural proteins with molecular weights of 49,000, 58,500, 69,000, and 98,000 were found, ant they were designated p49, p59, p69, and p98, respectively. There are several indications that p98 is a dimer of p49. The relative quantity of the structural proteins in a virion suggests that at least p49 (accounting for +/-70% of total protein mass) is a capsid protein and that there will be 12 capsomers per virion.

Animals↗
PubMed1976

[Zona ophthalmica and dendritic keratitis].

Two cases of herpes zoster ophthalmicus with dendritic keratitis are reported. Virological studies confirmed the double infection with herpes simplex type 1 virus in the corneal lesions and herpes zoster virus in the cutaneous lesions. We suggest the use of the immunoperoxidase test to identify the viral agent mainly because of its rapid and specific results. We are against the use of local steroids in dendritic keratitis unless the etiological agent is proved to be herpes zoster virus and not herpes simplex virus.

Aged↗