Biomedical subjects

E Kwapinski

Publications and source records attributed to E Kwapinski.

5 recordsLinked to original sources

PubMed1978

Studies on circulating gonococcal antibodies and antigens.

One hundred human sera obtained from acute gonococcal disease and 100 sera from nongonococcal diseases or healthy persons were concentrated four times and examined for the presence of circulating gonococcal antigens and antibodies by means of a counterimmunoelectrophoresis (CIE) and delayed hypersensitivity assay. Antibodies reacting with cytoplasmic gonococcal antigens were detected by CIE in 92% of sera received from patients suffering from acute gonococcal disease. Gonococcal antigens were found in the concentrated sera of 82.3% of patients on the basis of dermal reactions observed upon injections of these sera into the skin of rabbits sensitized with disrupted gonococci; 51.8% of the patients' sera gave delayed hypersensitivity reactions in rabbits sensitized with cytoplasmic antigens of N. gonorrhoeae. Control sera from healthy people and those with non-gonococcal diseases did not react with any of the preparations tested.

Antibodies, Bacterial↗

PubMed1978

Microbial-like antigens in human leukemia.

Antigens present in leukemic sera and organ extracts, but absent from non-leukemic sera and tissues, were detected by examining their reactions with anti-microbial antisera by means of counter-immunoelectrophoresis. Out of 110 different antisera used, 12 antisera, those produced against Micropolyspora, Coccidioides, Salmonella, Mycoplasma, Mycobacterium simiae, Absidia, pseudorabies virus and adenovirus, proved to react regularly with the leukemic sera. Absorption studies point to an immunological specificity of these cross-antigenicities. Interpretations and implications of these discoveries are discussed in the paper.

Antigens, Bacterial↗

PubMed1977

Immune responses to soluble antigens of treponemes.

Sonicates from five cultivable treponemes were used as antigens in delayed hypersensitivity tests and macrophage inhibition assays. Immunodiffusion analysis showed that the sonicate comprised two major antigenic components which were not distinguishable in the skin tests. The sonicate antigens elicited significant cell-mediated immunity in guinea-pigs. Treponema refringens biotype Nichols proved to induce the strongest delayed response. Delayed skin hypersensitivity to the antigens of Treponema pallidum was found in eight rabbits without orchitis, but not in six rabbits with T. pallidum orchitis. In contrast, the rabbits with syphilitic orchitis gave the strongest reactions with the non-pathogenic spirochetes. In terms of the cell-mediated immunity responses, Treponema phagedenis Reiter was found to be related to T. phagedenis Kazan 4 and Treponema denticola. Treponema scoliodontum was related to T. phagedenis Kazan 5, and T. refringens biotypes Nichols and refringens. The antigens of T. pallidum had the closest relationship to T. refringens biotypes refringens and Nichols ,T. phagedenis biotype Reiter, and T. scoliodontum. It was also demonstrated that some three of 12 human syphilitic sera reacted with the antigens of T. pallidum but not with control sera.

Animals↗

PubMed1976

Identification of gonococcal cultures by means of their cytoplasmic antigens and an anti-cytoplasm antiserum.

Soluble antigens were released by sonication from the cells of 60 primary cultures of Neiserria gonorrhoeae, 7 other species of neisseriae and 26 "mixed" cultures containing colonies of N. gonorrhoeae and other bacteria. Rabbits were injected with the soluble antigens of four strains of gonococci (clonal types 1 and 2), four other species of neisseriae and ten bacterial genera other than Neisseria. The antigens were tested against the non-absorbed antisera by a counter-immunoelectrophoresis method. It was found that the antiserum produced against the antigens of one N. gonorrhoeae strain, the strain GEK, reacted exclusively with the sonicates of all N. gonorrhoea cultures whether pure or mixed with other microorganisms. In contrast, the antisera to the antigens of all other N. gonorrhoeae strains cross-reacted with antigens of N. catarrhalis or N. sicca. Thus, the unique restricted reactivity of the anti-GEK antiserum permits its utilization as an immunological reagent for prompt identification of gonococcal cultures.

Antigens, Bacterial↗

PubMedDate not supplied

Preliminary investigations on abnormal immunoglobulin(s) in leprosy.

A new, abnormal immunoglobulin designated IgK has been discovered in leprous Rubino-negative sera. The IgK having a negative net charge and its own antigenic specificity appears to be partly related to the Fc fragment of IgA and to Fab fragments of known immunoglobulins, but its net charge is negative at pH 8.8. Its molecule seems to possess kappa but not lambda light polypeptide chains. Implications of this discovery are discussed.

Humans↗