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E L BIBERSTEIN

Publications and source records attributed to E L BIBERSTEIN.

17 recordsLinked to original sources
PubMed1963

ACTION OF HAEMOPHILUS CULTURES ON DELTA-AMINOLEVULINIC ACID.

Biberstein, Ernst L. (University of California, Davis), Patricia D. Mini, and Marjorie G. Gills. Action of Haemophilus cultures on delta-aminolevulinic acid. J. Bacteriol. 86:814-819. 1963.-Utilization of delta-aminolevulinic acid (ALA) by strains of Haemophilus recovered from various sources was investigated. With the 37 cultures studied, there was perfect correlation between absence of hemin requirement and ability to convert ALA to porphyrin. A total of 29 strains, including representatives of H. parainfluenzae, H. parahaemolyticus, H. "parasuis," and H. gallinarum, fell into this group. The remaining eight isolates, which were incapable of porphyrin synthesis from ALA, were strains of H. influenzae, H. suis, and a Haemophilus culture of uncertain classification obtained from a splenic abscess in a deer. In the active preparations, the products of synthesis included a mixture of porphyrins, porphobilinogen (PBG), and a pigment which absorbed light strongly at 470 to 480 mmu. Paper chromatographic studies of fractions of supernatants and cells revealed the presence of porphyrins having R(F) values similar to those of uro- and protoporphyrins, as well as some intermediate rates of migration probably representing coproporphyrins. Porphyrins were found both intra- and extracellularly, and PBG and the pigment absorbing at 470 to 480 mmu were confined to the supernatant.

Aminolevulinic Acid↗
PubMed1962

Oxidative metabolism of Haemophilus species grown at different levels of hemin supplementation.

Biberstein, Ernst L. (University of California, Davis) and Patricia D. Spencer. Oxidative metabolism of Haemophilus species grown at different levels of hemin supplementation. J. Bacteriol. 84:916-920. 1962.-Haemophilus strains were propagated on hemin-deficient and hemin-supplemented media and tested on various substrates for their ability to carry on oxidative metabolism. H. parainfluenzae and H. parahaemolyticus exhibited equally vigorousoxygen uptake under both methods of culture. Strains of H. influenzae showed substantially lower rates of oxygen uptake when cultivated in the absence of hemin than when grown in its presence. Some oxidative capacity remained, however, even when the organisms had been grown for several passages in the absence of hemin supplements.

Haemophilus↗