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E L Ruban

Publications and source records attributed to E L Ruban.

At least 19 recordsLinked to original sources
PubMedDate not supplied

[Fatty acid makeup of the lipids in soil and epiphytic yeasts].

The fatty acid composition of lipids was compared among yeast cultures belonging to the genera Rhodotorula, Lipomyces, and Cryptococcus. These lipids contain C10--C26 fatty acids, mainly with the even number of carbon atoms. Palmitic acid (C16 : 0) and oleic acid (C18 : 0) predominate. In the majority of the strains, the sum of unsaturated acids exceeds the sum of saturated acids. The content of unsaturated acids in the lipids of the epiphytic yeast Rhodotorula is higher than in the soil yeast Lipomyces. Besides C12--C18 acids, C22--C26 acids were identified by GLC at preset temperatures. Lignoceric acid (C24 : 0) was found for the first time in the cultures of Rhodotorula, Lipomyces, and Cryptococcus, and cerotinic acid (C16 : 0) was also detected in the Rhodotorula yeast. Fatty acids with a long chain are registered in the strains of Rhodotorula more often than in the strains of Lipomyces and Cryptococcus.

Chromatography, Gas↗
PubMedDate not supplied

[Comparative study of the properties of lipase of microbial origin].

A comparative study of properties of lipases of microbial origin has shown that the pH optimum of the enzymes from Geotrichum asteroides and Penicillium sp. is at pH 7.5 and 5.0, respectively. Lipase from Geotrichum asteroides appears more resistant to high temperatures and pH changes than the enzyme from Penicillium sp. Both enzymes split selectively vegetable oils, especially cotton and sunflower oils. Na cholate and deoxycholate at a concentration of 0.05-0.4% act as hydrolysis activators, particularly with respect to Geotrichum lipase. Na dehydrocholate does not show this effect.

Cholic Acids↗
PubMedDate not supplied

[Characteristics of a Mycobacterium mucosum culture oxidizing cholic acid].

Some physiologo-morphological and biochemical characteristics were studied with the culture of Mycobacterium mucosum 1210 which can assimilate cholic acid as a sole source of carbon. This culture is capable of spontaneous variability. Four variants of the culture were obtained which possessed different rates of the transformation of cholic acid. The variant which formed slimy colonies of pale-yellow colour was the most active. Some morphological modifications of the cells were investigated in the course of cultivation, such as the size, shape and formation of the capsules. Various growth media were tested, and the optimal values of pH were selected.

Cell-Free System↗
PubMedDate not supplied

[Conditions of exolipase biosynthesis by the fungus Oospora fragrans].

The fungal strain Oospora fragrans isolated from sunflower seeds had a high lipase activity, especially on the media containing soybean flour and various oils. The fungal lipase showed inductive characteristics, the best inducers being olive and soybean oils. The biosynthesis of lipase from O. fragrans occurred at the optimal temperature of 26-30 degrees C and strong aeration. Maximun of lipolytic activity did not coincide with maximum accumulation of biomass and developed at the log-phase of the fungal growth. With age the lipid content in the mycelium increased in parallel with the biomass accumulation. Mycelial lipids of O. fragrans contained nine fractions, among which fatty acids and triglycerides were in predominance. The study of properties of O. fragrans lipase showed a high pH value and thermal stability, the capacity to function at low temperatures, and activity towards many substrates. The enzyme readily hydrolyzed not only natural vegetable (olive, linseed and mustard) oils but also oils of microorganisms grown on petroleum hydrocarbons.

Enzyme Induction↗
PubMedDate not supplied

[Bacterial lipids].

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Bacillus subtilis↗
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Some properties of lipase from Geotrichum asteroides.

The optimal conditions for the manifestation of the lipase activity of an enzyme preparation from the culture liquid of the fungus Geotrichum asteroides were determined. It was found that the rate of hydrolysis depends on the substrate concentration, the enzyme concentration, and the reaction time. The lipase from G. asteroids is rather stable--it loses practically no activity in a year at 4 degrees C. The temperature optimum of the enzyme preparation is 37 degrees. The lipase from G. asteroides is most active at pH 8.0; it retains its activity when kept for 3 h in a reaction medium with a pH from 3 to 10.

Catalysis↗
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[Microbial lipases].

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Bacteria↗