Biomedical subjects
Publications and source records attributed to E Löhde.
Of 4023 Patients with gallstone disease, 124 were treated for common bile duct stones by endoscopy (ERC/EPT). Stone clearance was completed in 81% of the patients. Due to a morbidity of 5.9% and a mortality of 0.6%, the concept of preoperative ERC followed by laparoscopic cholecystectomy seems to reduce risk for the patients.
The laparoscopic cholecystectomy is fully integrated into the therapy concept of gallstone disease. The laparoscopic procedure was successfully performed in 97.5% of 2200 patients. In 2.5% of the patients the operation had to be converted to open surgery. Complications occurred in 3.6%, containing infections of the umbilicus (2.2%), postoperative bleedings (0.4%) and lesions of the d.hepatocholedochus (0.4%). Mortality rate was 0.1%. During the last 2 years the indication for the laparoscopic approach has been rapidly expanded while the indication for primary open surgery was reduced to 2% of the patients. Laparoscopic cholecystectomy proved to be a rapid and safe procedure for treating gallbladder stones.
Between 3/1990-3/1993 laparoscopic cholecystectomy was performed in 1750 patients in the mean age of 49.8 (7-83). In 48 patients (2.7%) the procedure had to be shifted to open surgery. The overall complication rate was 3.9%, the mortality 0.11%. The PDS-clip Absolok 300 was regularly used for occluding the cystic duct and the cystic artery. Clip-associated complications were never observed. The induced duct occlusion proved save even against operative manipulation. But special characteristics of the clip and its application had to be considered.
We report on the application of laparoscopic operative methods in ten patients with mechanical bowel obstruction and in seven patients with gastric perforation. From these experiences conclusions were drawn concerning the principles of trocar positioning as well as tactical and strategical considerations in acute abdominal surgery with known and unknown target regions.
In Germany, radioimmunoscintigraphy (RIS) (scintigraphic, specific imaging of benign and malignant diseases by radioactively labelled monoclonal antibodies) has been employed since 1985 in clinical trials involving patients with colorectal cancer. After proving successful for identifying primary tumors, RIS is now increasingly being used for the early diagnosis of recurrent and metastatic lesions. In this prospective study involving 75 patients with colorectal tumors confirmed by endoscopy and biopsy, it was shown that RIS using the SPECT technique with 99m-Tc-labelled monoclonal CEA antibodies has a sensitivity of 78% in the detection of local recurrent disease in cases in which diagnostic work-up has been inconclusive.
Radioimmunoscintigraphy (= RIS, scintigraphic "specific" imaging of benign and malignant diseases by means of radioactively marked monoclonal antibodies) has been performed in Germany in clinical studies since 1985 in patients suffering from colorectal cancer. After having been successfully proven in primary studies, RIS is now being used in the early diagnosis of recurrences and metastases. In the prospective study presented here the clinical usefulness of RIS was assessed in comparison against well-tried diagnostic methods including computed tomography in patients suffering from colorectal cancer. It was shown that RIS in SPECT technique (= single photon emission computed tomography) with 99mTc-labelled monoclonal CEA antibodies can visualise local recurrences if diagnostic findings are doubtful, with a sensitivity of 78% versus 50% for CT findings.
The prophylactic effect of intraoperative "one shot" antibiotic application in colorectal surgery was investigated. Patients were randomised and the antibiotic combination Mezlocillin/Metronidazole (group A) or Amoxicillin/Clavulamid acid (group B) was applicated in 160 patients. 111 patients were selected for the study (group A: 59; group B: 52 patients). In the postoperative course 53% (group A) and 67% (group B) of the patients developed bacterial infections. Abdominal wound healing was complicated by infection in 15% (A) and 12% (B) of the patients. A significant difference between the two groups could not be proven, 24% of all patients with documented intraoperative bacterial contamination and 10% of the patients with negative findings developed wound infections. In colorectal surgery patients are still at high risk for infectious complications. Applicated antibiotics should basically cover aerobic and anaerobic germs.
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Human colon carcinomas were operatively resected and the tumour-bearing segments interposed into an oxygenised ex vivo perfusion system. Pressure, flow, temperature, pH and metabolic parameters were controlled. Over a period of 45 min the 131I-labelled monoclonal antibody AUA1 was administered and its distribution in the tumour tissue analysed scintigraphically. The accumulated activity was determined in different tissues. The results showed that the AUA1 uptake increased with the degree of histological tumour differentiation. The main tumour:non-tumour ratio reached 0.8 in poorly, 4.1 in moderately and 5.9 in highly differentiated adenocarcinomas. Introducing the oxygenised erythrocyte-enriched perfusion media significantly increased the viability of the colon tissue. The ex vivo perfusion system will help to analyse factors determining monoclonal antibody accumulation in human colon carcinomas.
The acute-phase proteins CRP, haptoglobin, and sialic acid of 312 surgical patients (159 females and 153 males), aged between 18 and 95 years, were examined with a view to finding parameters for early prediction of perioperative septic complications. Laboratory tests were additionally applied to 39 patients for activated lymphocytes as well as for OKT3-, OKT4-, and OKT8-labelled T-lymphocytes. Straight forward postoperative decline in absolute numbers of OKT3- and OKT4-labelled T-lymphocytes was recordable from patients with septic complications, in contrast to patients with uneventful clinical courses. However, the OKT4 and OKT8 ratio did not yield any correlation with the clinical course. In acute-phase protein testing, a correlation was found to exist between CRP and clinical course. CRP levels in patients without septic complications (Group I) were found to depend clearly on the anatomic site of surgery. Normalisation of CRP levels was recordable from patients with primary septic disease and complete surgical management of the focus (Group II/1). Postoperative decline of CRP values was much less in patients with primary septic disease and incomplete surgical treatment (Group II/2). They had not even reached normality towards the end of observation. On average, unambiguously increased values were recordable as early as three days prior to clinical manifestation from patients with postoperative septic complications (Group III). Septic complications were found to occur along with re-increase or delayed decrease of CRP levels, after the fourth postoperative day. Sensitivity amounted to 78 per cent and specificity to 89 per cent.
We describe a model for the evaluation of anti-tumour antibody specificity, using a human carcinoma-bearing colon segment. After resection of the human colon tumour, the supplying artery was cannulated and perfused with fresh frozen plasma and heparin. Continuous control of pressure, flow, temperature, pH and various metabolic parameters were performed after administration of 131I-labelled anti-CEA antibody. Highly differentiated adenocarcinomas of the colon showed a much higher antibody uptake than undifferentiated tumours. Between 3 and 7% of the injected antibody was found in the tumour tissue. Autoradiography showed non-homogeneous binding in the tumour tissue. The non-specific antibody perfusion showed no tumour binding. We conclude that the ex vivo perfusion of resected colon carcinomas can be used to measure the kinetics of binding and clearance of MAbs in tumour tissue by direct scintigraphy. The cellular biodistribution of the antibody can be documented by means of autoradiography.
Skin tumours are classified by potential for causing disease and skin layer involvement. Primary diagnosis of skin tumours is established by escision biopsy, i.e. narrow excision of the tumour-adjacent tissue. Depending on the histological results, a secondary excision may be required. In this procedure a malignant tumour is assumed during the primary excision, which is performed according to "relaxed skin tension lines." Besides the fundamental interconnection of diagnostic and therapeutic principles the cosmetic-aesthetic aspects of skin tumour treatment are of utmost importance. Every operation performed for skin tumour follows the rules of plastic surgery.
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