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Biomedical subjects

E Laird

Publications and source records attributed to E Laird.

11 recordsLinked to original sources
PubMed1993

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Adaptation, Psychological↗
PubMed1992

Platelet-activating factor levels in human follicular and amniotic fluids.

Platelet-activating factor (PAF) was estimated in extracts of human follicular and amniotic fluids using a commercially available 125I-radioimmunoassay. Levels obtained before and after purification of the extracts by thin-layer chromatography were similar and PAF could be estimated with high accuracy and reproducibility over a wide dilution range. PAF levels in fluid aspirated from mature (17 mm average diameter) follicles from in vitro fertilization patients were 1005 +/- 129 fmol/ml in successful (clinical pregnancy) cycles and 949 +/- 75 fmol/ml in unsuccessful (failure of implantation) cycles. PAF levels were also similar in follicles containing eggs and those where no egg was found, and in fluid from follicular cysts characterised by low oestradiol and progesterone levels. PAF was detected in midtrimester amniotic fluid (60 +/- 20 fmol/ml). At term, PAF was low or undetectable in amniotic fluid obtained at elective caesarean section before the onset of labour, but increased to 102 +/- 28 fmol/ml in samples obtained following spontaneous labour and vaginal delivery. The data suggest that PAF may be involved in ovulation and parturition but further studies into the mechanism of action of PAF are necessary. The good performance of the radioimmunoassay and the convenience of gamma-counting are strong advantages of this method over the available bioassays, but its expense remains a drawback.

Amniotic Fluid↗
PubMed1989

Selective inhibition of angiotensin-II-mediated aldosterone secretion by 5-hydroxyeicosatetraenoic acid.

We have previously demonstrated that the 12-lipoxygenase (12-LO) pathway plays a key role in angiotensin-II (AII)-dependent aldosterone production. In the present study we examined the role of the 5LO pathway on AII-induced aldosterone secretion in rat glomerulosa cells in vitro. The 5LO product 5-hydroxyeicosatetraenoic acid (5HETE) and its unstable precursor 5-hydroxyperoxyeicosatetraenoic acid did not significantly alter basal aldosterone secretion in concentrations from 10(-9)-10(-7) M. In contrast, 5HETE reduced peak AII-induced aldosterone production from 59.1 +/- 9.0 to 37.96 +/- 7.2 ng/10(6) cells (P less than 0.01). This was accompanied by inhibition of the AII-stimulated rise in 12HETE production (10(-9)M AII, 160 +/- 4% of control; 10(-9) M AII plus 10(-7) M 5HETE, 90 +/- 1% of control production). However, 5HETE had no effect on the aldosterone response to potassium or ACTH, secretagogues that cause no activation of the 12LO pathway. These results suggest that the 5LO product 5HETE can selectively modulate AII-dependent aldosterone secretion. Further, the selective inhibitory effect of 5HETE on the AII effect in rat glomerulosa cells may be exerted by blockade of arachidonate metabolism via the 12LO pathway. These results suggest that the 5LO pathway may negatively modulate AII action in the adrenal zona glomerulosa.

12-Hydroxy-5,8,10,14-eicosatetraenoic Acid↗
PubMed1986

Twin pregnancy and parturition in a patient with the Bernard Soulier syndrome.

A patient with the Bernard Soulier Syndrome was successfully delivered of twins. The pregnancy was complicated by slight bleeding from the uterus during the first 8 weeks, by cord prolapse necessitating emergency caesarean section, and by moderate post partum haemorrhage. The management of pregnancy in the Bernard Soulier Syndrome is discussed.

Adult↗
PubMedDate not supplied

Hitting the moving target of program choice.

The changes in what 2,927 people wanted and got for programs in 1988 were compared with what 3,934 people wanted and received in 1996 in Wyoming and South Dakota. As recommendations change, they present a "moving target" for these service-delivery systems. In 1988, 51% of those served in Wyoming and 62% of those served in South Dakota were in their preferred setting. By 1996, the Wyoming percentage rose to 84%, whereas South Dakota's percentage moved up to 72%. These increases enhanced other "quality of life" measures. Comparing 1996 with 1988, for example, fewer individuals in both states reported no social or leisure activities. Fewer reported the lack of transportation as a barrier and fewer reported having no one to accompany them to activities. More individuals reported family contact, family visits, and identified a hobby or personal leisure activity. The 8-year expansion of services and supports had increased positive social activities.

Adult↗