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E Leenders

Publications and source records attributed to E Leenders.

14 recordsLinked to original sources
PubMed1990

[Not Available].

Explore the source record for details and available documents.

Digestive System Diseases↗
PubMed1989

Typing of Aeromonas strains by DNA restriction endonuclease analysis and polyacrylamide gel electrophoresis of cell envelopes.

The outer membrane protein (OMP) composition (OMP typing) of 46 fecal Aeromonas strains from hybridization groups (HGs) 1 (A. hydrophila; n = 10), 4 (A. caviae; n = 16), and 8 (A. veronii; n = 20) were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a phenotypic typing method. Almost every isolate of HG-1 and HG-8 had a unique OMP profile, in contrast to isolates of HG-4, which were separated into five different OMP types. It was possible to recognize HGs 1, 4, and 8 by OMP profiles. Twenty-three Aeromonas strains from HGs 1 (n = 5), 4 (n = 10), and 8 (n = 8) were tested by whole-cell DNA restriction endonuclease analysis (REA) as a genetic typing method. All strains tested by REA (with SmaI) had different DNA digestion patterns. Although additional DNA-rRNA hybridization analyses with SmaI and 16S and 23S rRNAs from Escherichia coli showed a reduction in the number of restriction bands to 8 to 13 hybridized fragments, the discriminative value was less when compared with that obtained by REA. The individual differences found by REA were used to analyze whether patients remained colonized by the same Aeromonas strain. Of 11 patients with diarrhea, 2 had a different isolate on repeat culture. In addition, one of nine tested fecal samples contained two Aeromonas isolates with different REA patterns. These results indicate that during diarrheal disease the intestinal tract may be colonized simultaneously with different Aeromonas isolates.

Aeromonas↗
PubMed1984

[Neck cysts and fistulas. Embryological rest structures].

During the fourth week the human embryo develops a group of five paired arches at the cranial end. These are the branchial arches, soon completed by the formation of four ectodermal invaginations or branchial clefts and four endodermal branchial pouches. These primary organs will mostly disappear; the remaining form the definite head and neck structures. The congenital persistance of abnormal tissue is at the origin of congenital neck anomalies known as branchial cysts or fistulae and the thyreoglossal cyst or fistula. The localisation and course of these fistulae based on their embryological origin are predictable. The diagnosis is essentially clinical. The treatment is early surgical resection.

Branchial Region↗
PubMedDate not supplied

[The value of anorectal sphincteromyectomy for the diagnosis and treatment of chronic constipation in children].

From January 1974 till the end of 1981, 105 children with chronic constipation not responding to any medical treatment, were submitted to anorectal myomectomy. This operation has both a diagnostic and a therapeutic value; the histological study of the muscle biopsy can confirm or not a Hirschsprung's disease. It is also an ideal treatment in cases of achalasia and idiopathic chronic constipation. If necessary a secondary rectosigmoidectomy can be performed. We did not have any important complication, none of our patients had soiling. Eighty-eight percent of our patients had regular bowel movements after the anorectal myomectomy with a better individual, social and family life.

Adolescent↗
PubMedDate not supplied

[Imperforate anus. Principles and methods of surgical treatment].

Imperforate anal surgery is not only concerned with survival but also with the problem of incontinence. The ideal long term result must be fecal continence without constipation or fecal soiling. As a result of embryological studies new anatomical and physiological concepts form the basis of our present knowledge concerning diagnosis and surgical approach. A new classification of anorectal anomalies evolved in 1970. It is based on the anatomy of the visceral deformities in relation to the principal muscle of continence, the puborectalis part of the levator ani muscle. The primary divisions are those of the high rectal lesions above the levator muscle (supralevator), and those of the low anal lesions through the levator (translevator). The translevator type will be corrected through a perineal approach. The prognosis is excellent. The supralevator type needs a combined abdominoperineal operation. The continence prognosis of this type still raises problems. Only a good knowledge of these basic concepts and a careful dissection of every sphincteric muscle will ameliorate our results.

Anus, Imperforate↗