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E Lonc

Publications and source records attributed to E Lonc.

35 records · Page 2Linked to original sources

[Insecticidal activity of various strains of Bacillus against larvae of houseflies (Musca domestica)].

Insecticidal activity of B. thuringiensis subsp. morrisoni and B. thuringiensis subsp. darmstadiensis as well as B. sphaericus 2362 and 2297 was evaluated against larvae of the house fly (Musca domestica) under laboratory condition. The activity of all tested strains was similar, LC50 ranges from 4 x 10(7) to 1 x 10(8) spores/ml. The higher spores concentration (2 x 10(9)) caused ca 70 per cent mortality. The relative low sensitivity of house fly larvae could results from weakly alkalic pH of the gut (7.6-7.8).

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Intraspecific morphometric variability of Goniocotes chrysocephalus (Phthiraptera, Ischnocera).

Morphometric variability of G. chrysocephalus was analysed and compared between and within the groups of individuals collected from single pheasant host (idiohostal populations) and from great number of hosts belonging to the same species (synhostal). The congruence of ranges of variation of 4 characters from 3 idio- and 1 synhostal population was examined. The arithmetic means of head width and abdomen as well as total body length of males from 2 idiohostal populations were statistically different from synhostal one. On the other hand, in the females such differences were only in the head width of idiohostal populations.

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Insecticidal activity of Bacillus thuringiensis subspecies against Menopon gallinae (Mallophaga: Menoponidae).

10 Bacillus thuringiensis strains were used to determine the susceptibility of Menopon gallinae (L). In order to identify B. thuringiensis from dead insects, previously exposed to bacteria, the streptomycin-resistant mutants of strains were also applied. The experiments were conducted in a group of 20 specimens, in three replications. The absorbtion at 750 nm directly corresponded to the concentration of 23 X 10(6) spores/ml in each assay. The biting lice were exposed on agar plates, inoculated by 0.2 ml of B. thuringiensis preparations dipping for 2, 4 and 8 s or spraying with 0.5 ml of adequate strain. Laboratory trials showed that M. gallinae was susceptible to toxic activity of all tested strains. While the susceptibility to various serotypes varied, depending on the time and mode of infection, 3 subspecies: kurstaki, finitimus and kenyae were usually the most active. The least active strains were the subsp. galleriae and aizawi. The microbiological examination of dead mallophaga treated with B. thuringiensis proved the presence of this bacterium in smears taken from the insects. A comparison of morphological as well as biochemical properties of the antibiotics sensitive and the antibiotics-resistant mutants revealed no difference.

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Susceptibility of poultry biting lice (Mallophaga) to Dipel and Bacilan (Bacillus thuringiensis).

Dipel (Abbott Laboratories, N. Chicago, U.S.A.) and Bacilan (PPR, Wałcz, Poland), commercial preparation of Bacillus thuringiensis var. kurstaki, containing 16,000 IU/mg were tested in vitro on Menopon gallinae (L.) and Eomenacanthus stramineus (Nitzsch). The insects were placed in feathers, in Petri plates, and sprayed with 0.25, 0.5, 0.75, 1, 2, 4 and 10% solutions of insecticides. Each concentration was tested on a group of 10 insects, in three replications. The viability was observed after 10, 20, 30, 60 min and then 10 times within 1 hr intervals. Dipel was effective in lower concentrations [LC50 = 2.2 (1.4-3.5)% against E. stramineus and 2.9 (1.8-4.6) against M. gallinae]. LC50 of Bacilan were 2.9 (1.8-4.3) for E. stramineus and 3.2 (2.5-4.0)% for M. gallinae respectively. The ingestion of microbial insecticides was proved by the presence of spores B. thuringiensis in the insects being previously exposed to indirect activity of 2.2% Dipel and 2.9% Bacilan. Moreover, the growth of B. thuringiensis colonies in the lice culture was observed. 3% Dipel solution proved to be effective in killing the insects on the host, but no earlier than after the second application within 24 hr intervals.

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Connections between poultry biting lice and microflora.

Microorganisms were found in about 300 Mallophaga ( Menopon gallinae, Eomenacanthus stramineus , Goniocotes gallinae) and 11 host hens. The majority of isolated bacteria was determined as grampositive cocci. The microflora found on the surface of and inside the Mallophaga as well as in the samples of hen's skin did not exhibit significant qualitative differences. The internal microflora in Mallophaga appeared qualitatively a little poorer than the external. Breeding of bacteria from interior tissues of nymphs of G. gallinae seldom succeeded, although bacteria were found in all microscopical preparations. The results indicate the bacteriostatical effect of an unknown agent in the digestive system of Mallophaga, as well as the presence of microorganisms (probably specific to the insects investigated) which, however, cannot be distinguished by the usual methods.

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The possible role of the soil fauna in the epizootiology of cysticercosis in cattle. I. Earthworms -- the biotic factor in a transmission of Taenia saginata eggs.

The role of earthworms in the transmission of Taenia saginata eggs was determined by inoculating 30 earthworms directly: lumbricus terrestris, Allolobophora caliginosa and Eisenia foetida with tapeworm eggs. The remaining earthworms (280 specimens) were examined by contaminating their medium indirectly with T. saginata eggs or segments. It has been concluded that the eggs of tapeworms may be transmitted by the digestive tracts of earthworms. It seems that in the natural environment earthworms may contribute to the horizontal and the vertical spreading eggs in soil and the same may play an important role in the epizootiology of cysticercosis in cattle.

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The possible role of the soil fauna in the epizootiology of cysticercosis in cattle. II. Dung beetles--a biotic factor in the transmission of Taenia saginata eggs.

In the present study 510 specimens of dung beetles (Aphodius fimetarius, A. fossor, Sphaeridium scarabaeoides) were examined after exposure to a medium contaminated with Taenia saginata eggs or segments. It has been demonstrated that eggs of tapeworm may be transmitted by the digestive tracts of dung beetles as well as on the surface of Aphodius fimetarius. The above findings suggest that dung beetles, likewise in nature the earthworms, may play an essential role in the spreading eggs of T. saginata. Especially Aphodius fimetarius, because of its passive transmission of eggs on long distances, may be involved in the epizootiology of cysticercosis in cattle.

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