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Biomedical subjects

E M Khalilov

Publications and source records attributed to E M Khalilov.

At least 19 recordsLinked to original sources
PubMed1995

[Free and esterified epidermal cholesterol in psoriasis].

The levels of epidermal total, free and esterified cholesterol were studied in psoriatic patients. Lipids were isolated from the areas of psoriatic lesions and from the normally appearing epidermis by the authors' non-invasive surface extractive method. The quantity of cholesterol was given in microgram per square centimeter. Fifty six and 11 healthy persons were examined. The skin cholesterol levels were twice-four-fold higher in the patients than in the healthy subjects. The same levels were noted from the areas of lesion and from normally appearing epidermis. The proportion of an esterified fraction decreased mainly in the normally appearing epidermis areas, especially in severe psoriasis. The role of the findings was also discussed.

Adolescent↗
PubMed1994

[The effect of cholesterol level in liposomes on the interaction with blood serum lipoproteins].

The effect of the cholesterol content in liposomes on their interaction with blood serum lipoproteins has been studied. It was shown that phosphatidyl choline liposomes without cholesterol did not interact selectively with any class of serum lipoproteins. At the same time, addition of high cholesterol concentrations to liposomes confers to them a new feature, namely the ability to interact selectively with the serum low density lipoproteins. Up to 90% of such lipoproteins can interact with liposomes. This finding was confirmed by "in vivo" data. The observed effect of "address" properties given to liposomes by a native lipid may be of interest in search for new possibilities to use liposomes for selective delivery of drugs.

Cholesterol↗
PubMed1993

[The antiatherogenic action of plant oils with added omega-3 polyunsaturated fatty acids].

Quick model of experimental atherosclerosis--3 weeks of cholesterol feeding and 3 weeks of standard diet for aorta atheromatosis development--was used in rabbits. Then one group of animals was fed 1 month by plant oil of specific composition, with added omega-3 (linolenic) fatty acid, and the other group received the same dose of fish oil. The most intensive decrease of plasma cholesterol and erythrocyte cholesterol/phospholipid ratio was in rabbits, that received the oil with linolenic acid. Triglyceride level decreased gradually in all animal groups in the same degree, and HOL cholesterol did not change. After the end of experiment the aorta damage degree in group, that received plant oil with linolenic acid, was only 10.8%, as compared with 36.2% in control group (without treatment) and 39.3% in rabbits, that received fish oil. The absence of fish oil effect supports the earlier data about rabbits specific sensitivity to this product resulting in possible hepatotoxic effect. The data indicates on the usefulness of rabbit model in fish oil effects investigations, and also on the positive, antiatherogenic effect of plant oil with added linolenic acid.

Animals↗
PubMed1993

Interaction of cholesterol containing liposomes with blood serum lipoproteins.

The interaction of liposomes containing different amounts of cholesterol with human serum low density lipoproteins was investigated. Efficiency of the interaction depended on the cholesterol content of liposomes and was highest for liposomes with the maximal cholesterol/phospholipid molar ratio. The latter selectively and effectively interacted with low density lipoproteins, with up to 90% of lipoprotein particles interacting with liposomes in serum. Fusion of the particles with liposomes was observed during the interaction.

Cholesterol↗
PubMed1992

[Specificity of the effect of polyene phosphatidylcholine depending on the mode of administration and animal species].

The effects of polyene phosphatidylcholine (PPC) treatment at oral and intravenous administration to rats and rabbits in hypercholesterolemic diet were studied. No aorta damage was observed in either of rat groups. But fatty liver appeared, and it was the greatest in rats, who received cholesterol and PPC. The result may be attributed to adaptive protection of peripheral tissues due to high experiment duration (18 months) in the state of active reverse cholesterol transport (RChT). No antiatherogenic effect was noted in rabbits at PPC administration (170 mg/kg), while its intravenous injection (50 mg/kg) resulted in marked reduction of plasma cholesterol level, elevation of HDL cholesterol and decrease of the extent of aorta damage. The conclusion is drawn on the ppc high antiatherogenic effect predominantly at intravenous administration, and on advisability of its use in cases of RChT deficiencies, as its activator.

Administration, Oral↗
PubMed1992

[Antiatherogenic action of corn oil in experimental atherosclerosis].

Therapeutic effect of corn oil was studied in rabbits with alimentary atherosclerosis. Corn oil was administered (2 ml/kg, 30 days) after the completion of cholesterol diet unlike studies, where they were administered simultaneously. Total cholesterol, apoB-lipoprotein cholesterol and triglycerides decreased more intensively in rabbits fed by corn oil than in control group. No changes in high-density lipoprotein cholesterol were observed. The most pronounced effect was noted in aorta morphological analysis: an aorta damage degree was 4.8% as compared with 52.9% in the control group. The results show that available plant oils with omega-6 polyunsaturated fatty acids (PUFA), in particular corn oil, may as well as omega-3 PUFA be used as the base for antiatherogenic preparations.

Animals↗
PubMed1992

[Content of cholesterol extracted from human skin surface--a possible discriminant of atherosclerosis?].

Basing on the data of Bouissou et al. on an increase of cholesterol content in the skin biopsies in atherosclerosis patients, the cholesterol amount was evaluated after superficial atraumatic rapid treatment of patients palm skin. The optimization of the extraction conditions was performed: the surface skin cholesterol value was constant for individual subjects (variability factor 5%). The range of this value was 1.5-6 Mg/cm2 and it was higher for atherosclerotic patients. No correlations with any conventional plasma lipoprotein parameters were observed. At the same time positive correlation (r = 0.54. p < 0.01) was observed with dyslipoprotein coefficient--(Ch+TG/alpha-Ch). Possible diagnostic significance of this skin cholesterol parameter is discussed.

Biopsy↗
PubMed1991

Diet-induced hypercholesterolaemia in the rabbit.

Intraspecies variation in diet-induced hypercholesterolaemia in rabbits, simulating atherosclerosis, was studied. The chinchilla rabbit population examined contained several subpopulations, as indicated by polymodal forms of histograms of plasma cholesterol levels. This finding indicates that the inclusion of subpopulations in an investigation can lead to erroneous conclusions, and that subpopulations should be identified before such work is undertaken. The relationship between the molar cholesterol/phospholipid ratio in rabbit erythrocyte membranes and the plasma cholesterol level in experimental atherosclerosis was also studied. A correlation was evident only over the range of 1-5 g cholesterol per litre of plasma.

Animals↗
PubMed1990

Cholesterol oxidation on fluorocarbon emulsion surface leads to the formation of 7-peroxycholesterol.

Formation of biologically active oxidized derivatives of cholesterol as a result of its oxidation on the surface of fluorocarbon emulsions was studied. A single product of cholesterol oxidation, 7-peroxycholesterol, was found. It was shown that 7-peroxycholesterol and its derivative 7-keto-cholesterol inhibit the rosette formation between human T-lymphocytes and sheep erythrocytes. These substances exert a strong cytostatic action on the growth of procaryotic and eucaryotic cell cultures. Thus, oxidative modification of blood plasma components on the surface of fluorocarbon emulsion particles with the formation of highly active compounds must be taken into account when using the fluorocarbon emulsions in medicine.

Acholeplasma laidlawii↗
PubMed1990

[Formation of biologically active substances during cholesterol oxidation on the surface of fluorocarbon emulsions].

Oxidative modification of cholesterol on the surface of fluorocarbon emulsions was studied. The oxidation yielded one primary product--7-peroxycholesterol. It was shown that the obtained cholesterol C7 derivatives possess a high biological activity. It was concluded that the possibility of oxidative modification of plasma substances on the surface of fluorocarbon emulsion particles with the formation of highly active compounds must be taken into account when using the fluorocarbon particles in medicine.

Cholesterol↗
PubMed1989

[The effect of liposomes containing cholesterol or 7-ketocholesterol on the capacity of cultured cells to form colonies].

The experiments with the cultivated Chinese hamster cells showed that the incubation of cells with (phosphatidylcholine+cholesterol+7-keto-cholesterol)--containing liposomes (4:3:1 by weight) during two hours leads to a decrease in the effectiveness of colony-forming up to zero, while the (phosphatidylcholine+cholesterol-containing liposomes (1:1 by weight) reduce the effectiveness by 90%. Furthermore, the cholesterol-containing liposomes cause the decrease of the number of colonies with maximal size, accompanied by the increase of the number of colonies with middle size.

Animals↗
PubMed1989

[Changes in the levels and composition of lipoproteins and apolipoproteins of blood plasma in patients with ischemic heart disease after hemosorption].

Prior to and following activated charcoal hemosorption, concentrations of lipids, apolipoproteins AI and B and lipid and protein composition in lipoprotein fractions isolated by ultracentrifugation were determined in the plasma from patients with coronary heart disease. The majority of the patients showed a parallel proportional decrease in plasma atherogenic parameters and all components of very low density lipoproteins and low density lipoproteins, triglycerides in particular. Antiatherogenic parameters, such as high density lipoprotein and apo-AI cholesterol, and all the components in high density lipoprotein subfractions were less reduced. In 19% of the patients, hemosorption failed to affect plasma lipids and apolipoproteins. The findings suggest that, in case of successful hemosorption, apoB-containing lipoproteins are chiefly eliminated as whole complexes from the plasma and that this procedure is most beneficial in hypertriglyceridemia.

Apolipoproteins↗