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E M Kuznetsova

Publications and source records attributed to E M Kuznetsova.

14 recordsLinked to original sources
PubMed1992

[A comparative study of the expediency of using different chemical methods for determining protein in allergen preparations].

Parallel use of some known chemical procedures for measurements of protein in commercial forms of allergens revealed considerable variations in the results, which was due to the nature of the methods used and to admixtures of different substances in the preparations under study. The conclusion on the necessity of using at least two methods of protein determination for the standardization of allergens was made. Preference was given to Bradford's method. As the standardization of allergens manufactured in the USSR is carried out on the basis of the quantitative determination of protein (in PNU), this problem is of not only theoretical, but also practical importance.

Allergens↗
PubMed1990

[An immunoenzyme system for determining antibodies to Streptococcus pneumoniae polysaccharides in biological fluids].

The enzyme immunoassay (EIA) system for the determination of antibodies to capsular polysaccharides of pneumococci, serotypes 1, 3, 6B, 8, 9N, 15F, 23F, and C-polysaccharide has been developed on the basis of poly-L-lysin-modified antigens. The use of isotype-specific conjugates in this system permits the detection of IgG and IgA antibodies in different biological fluids: blood serum, pleural fluid, saliva, milk. Samples obtained from children with pneumococcal infection and from nursing mothers have been studied. As shown in this study, the EIA system can be used for the evaluation of the dynamics of pneumococcal infection in children.

Adolescent↗
PubMed1988

[Evaluation of the level of antibodies to purified meningococcal antigens].

Group B meningococcal antigens, such as polysaccharide, lipopolysaccharide, protein preparation, as well as sonicates obtained from meningococcal cells, groups A, B and C, have been isolated. On the basis of these preparations the parameters of an enzyme immunoassay system for the detection of antibodies to individual meningococcal antigens have been established, and the specificity of the system and the possibility of using it for the evaluation of the level of antibodies to meningococci in human sera have been studied.

Animals↗
PubMed1987

[Possible serodiagnosis of pertussis infection by an immunoenzyme method].

The sera of children and adults with a history of pertussis, as well as the sera of children immunized in three injections, have been studied in the enzyme immunoassay. The levels of antibodies to Bordetella pertussis protein and lipopolysaccharide, and to disintegrated B. pertussis cells have been determined; a serum titer of 1:1,600 and higher is considered as a criterion for the serological diagnosis of pertussis.

Adult↗
PubMed1984

[Type-specific pneumococcal agglutinating sera in precipitation tests].

Diagnostic agglutinating sera to pneumococci of different serotypes have been studied with respect to their capacity for taking part in the reactions of precipitation with capsular pneumococcal polysaccharides. The sera have proved to be highly active and specific in the reactions of double immunodiffusion and immunoelectrophoresis. The sera under study have also been found to react with cattle serum, one of the components of the medium used for the cultivation of pneumococci.

Agglutination↗
PubMed1983

[Immunochemical study of the type-specific polysaccharides of pneumococcus].

Polysaccharide-containing preparations obtained from pneumococci, serovars 3, 6B, 9N, V and 23F, were studied by the methods of immunodiffusion and rocket immunoelectrophoresis (RIE). The tests were carried out with the use of rabbit antisera to formol vaccines prepared from serovars 3, 6, 9, 10, 12, 14, 18, and 23. The highly purified preparations of each of these serovars were shown to possess strict serological specificity. The results of RIE and chemical analysis permitted the selection of the preparations which served as reference antigens for 5 above-mentioned pneumococcal serovars. The calibrating curves plotted with the use of the reference antigens helped calculate the relative quantitative content of specific polysaccharide in preparations of different batches. The antigenic preparations were shown to differ in the content of specific polysaccharide and the degree of their purification. The authors recommend that double immunodiffusion and RIE should be used in combination in the study of the serological specificity of pneumococcal polysaccharide-containing preparations. The latter may be used as a rapid method for the standardization of the above-mentioned pneumococcal preparations and their mixtures. To achieve greater objectivity in the analysis of the mixtures, the use of sectional RIE, a variant of RIE proposed by the authors, is probably expedient. The present work also shows the possibility of using RIE for evaluating the level of the accumulation of specific polysaccharide during the cultivation of pneumococci in a liquid culture medium.

Immunochemistry↗
PubMed1981

[Protective activity of antigenic preparations isolated by different methods from Streptococcus pneumoniae serotype 3].

The specific protective activity of antigenic preparations isolated from Str. pneumoniae culture by sedimentation with 96% ethanol or acetone was demonstrated in experiments with the active immunization of mice and their subsequent challenge with a virulent culture. The protective activity of antigenic preparations, expressed in micrograms, was shown to be directly related to the protein content of these preparations and inversely related to their carbohydrate content. The deproteinization of antigenic complexes isolated from Str. pneumoniae grown both in solid and in liquid culture media was accompanied by an increase in their immunogenicity.

Animals↗
PubMed1981

[Physicochemical and immunochemical characteristics of the polysaccharides of Streptococcus pneumoniae serotype 3].

Capsular polysaccharides were isolated from Str. pneumoniae (serotype 3), grown on solid culture media, by the method of acetone sedimentation and subsequent deproteinization with phenol. The preparations thus obtained possessed serological type specificity, contained more than 40% of carbohydrates and insignificant amounts of protein and nucleic substances. The isolated polysaccharides contained a high-molecular fraction with the coefficient of distribution not exceeding 0.1, thus corresponding to the requirements for highly immunogenic preparations.

Chemical Phenomena↗
PubMedDate not supplied

[Changes in the number of DNA-protein cross links in spleen lymphocytes of mice exposed to low intensity low dose gamma irradiation].

Levels of DNA-protein cross-links (DPC) and DNA single-strand breaks (SSB) in spleen lymphocytes were studied in mice exposed to low-intensity gamma-radiation (1.7 mGy/day) for 1, 4, 10, 20, and 30 days. The spleen mass and count of lymphocytes isolated from this organ also has been investigated. The significant increase in the DPC level as compared to the control occurred on the 10-th and 30-th days of irradiation at doses of 1.7 and 5.1 cGy, accordingly. The number of spleen lymphocytes normalized to organ mass significantly decreased on the 4-th and 30-th days of the experiment. No increase was found in levels of alkali-labile sites and SSB. In contrast, the increase in the amount of duplex form DNA was recorded on the 4-th and 30-th days of the experiment. Our indicate that DPC formation after irradiation at low doses represents some form of cellular response to the damaging agent.

Animals↗