Biomedical subjects

E M Torello

Publications and source records attributed to E M Torello.

3 recordsLinked to original sources

PubMed2000

Treatments resulting in pregnancy in nonovulating, hormone-treated oocyte recipient mares.

Synchronization of follicle growth between oocyte donor and recipient mares is difficult. To avoid this, recipient mares in a clinical program were used during a period of low follicular activity, and were treated with estrogen before transfer and progesterone after transfer. Five pregnancies were established after oocyte transfer to nonovulating, hormone-treated recipient mares. One pregnancy was lost before 30 d gestation, and the other 4 foals were carried to term. One foal died at birth. Establishment and maintenance of pregnancy in these mares indicates that nonovulating, hormone-treated mares may offer an alternative to cyclic recipients in oocyte transfer programs.

Animals↗

PubMed1999

Birth of a foal after oocyte transfer to a nonovulating, hormone-treated recipient mare.

A nonovulating, hormone-treated mare was used successfully as an oocyte recipient. The mare's ovarian activity was suppressed using progesterone and estrogen treatment. This treatment was stopped, then estrogen was administered for 3 d prior to the transfer. An oocyte was recovered from the follicle of a donor mare and was transferred via flank laparotomy into the recipient's oviduct. The recipient mare was inseminated 7 h before transfer. The recipient was treated with intramuscular progesterone from the day after transfer until 47 d after transfer, and then with oral altrenogest until 150 d gestation. A normal colt was born at 321 d gestation, and was shown by DNA analysis to be the progeny of the donor mare. This is the first report of fertilization and embryo development to term after transfer of oocytes to a nonovulating mare, and, to our knowledge, the first of its kind in any domestic species.

Animals↗

PubMed1998

Oocyte transfer in mares.

Oocytes were collected from dominant preovulatory follicles of donor mares 24 hours after administration of human chorionic gonadotropin. Oocytes were incubated in vitro for 12 or 18 hours before transfer to recipient mares, representing maturation times after human chorionic gonadotropin administration of 36 and 42 hours, respectively. Pregnancy rates after transfer were 4 of 5 in the 36-hour group and 2 of 3 in the 42-hour group. The overall pregnancy rate achieved (6/8 mares) indicated that oocyte transfer may be useful clinically.

Animals↗