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Biomedical subjects

E Mandache

Publications and source records attributed to E Mandache.

At least 19 recordsLinked to original sources
PubMed1989

The ultrastructure of nuclear inclusions in the giant-cell tumor of bone.

The ultrastructural study of three cases of giant-cell tumor of bone showed two types of nuclear inclusions. The first type, common to giant cells and stromal cells was granular, and sometimes associated with a fibrillar pattern observed only in giant cells. The second type was represented by nuclear bodies, encountered mostly in the stromal cells. The nuclear bodies were made up of a granular core enveloped in a multilayered coat. The nature of these nuclear inclusions might be viral and thus a viral pathogenesis of the giant-cell tumor of bone is discussed.

Adult↗
PubMed1981

Simultaneous detection of two different cell surface antigens by electron microscopy by means of multivalent hybrid antibody with double specificity.

Multivalent hybrid antibody (MHA) complexes with dual specificity were prepared by combining two antibodies of different specificities, one against ferritin (Fer), the other against horseradish peroxidase (HRP), with protein A of Staphylococcus aureus (SpA). Electron microscopy of mouse spleen lymphocytes and thymocytes (previously coated with mouse IgG anti-Thy-1 antibody) treated with IgG anti-Fer/SpA/IgG anti-mouse Ig complex and Fer gave better resolution and higher accuracy than previously obtained with IgG anti-HRP/SpA/IgG anti-mouse Ig complex and HRP (Mandache et al., 1980). Surface Thy-1 alloantigen and Fc receptor (charged with human IgG) treated with a mixture of IgG anti-Fer/SpA/IgG anti-Thy-1 and IgG anti-HRP/SpA/IgG anti-human Fab could be simultaneously detected on the thymocyte surface by either light or electron microscopy using Fer and HRP. The concomitant visualization of Thy-1 alloantigen (with Fer) and FcR (with HRP) on mouse thymocyte clearly shows that their distribution is largely independent and that the amount of Thy-1 antigen is greater. These results show that electron microscopy with a mixture of MHA is a useful technique for simultaneous location of two antigenic markers on the cell surface.

Animals↗
PubMed1980

Multivalent hybrid antibody with double specificity as a tool for locating cell surface antigens by electron microscopy.

Multivalent hybrid antibody complexes with dual specificity were prepared by combining rabbit antibody with anti-mouse immunoglobulin (mIg) and anti-peroxidase (HRP) specificity using protein A of Staphylococcus aureus. The presence of two antibody molecules with anti-mIg and anti-HRP specificity in a single molecule of hybrid complex was demonstrated by their abilities to produce hemagglutination with both HRP-coated and mIg-coated sheep red cells, to give a reaction of complete identity with mIg and HRP and to allow mIg bearing lymphocytes to form rosettes with HRP-coated sheep red blood cells. Electron microscopy of mouse lymphocytes and thymocytes (previously coated with mIg anti-Thy-1 antibody) treated with hybrid antibody complex and HRP showed strong and specific staining of the cell membrane of both cell types. The hybrid antibody complex containing anti-HRP antibody is a valuable reagent for determining various antigenic markers on cell membranes by electron microscopy.

Animals↗
PubMed1978

I. An ultrastructural investigation on erythrocytes and lymphocytes coated with proteins: use of CrCl3 as an electron-dense marker.

Proteins (human immunoglobulin G and protein A of Staphylococcus aureus) coupled to the surface of erythrocytes (sheep, mice, man and chicken) and to mouse spleen lymphocytes by chromic chloride technique acquire electron-dense properties and may be visualized in electron microscopy. Sheep red blood cells coated with rabbit anti-erythrocyte antibodies and treated with chromic chloride showed continuous electron-opaque labelling of the cell membrane due to chelation of rabbit IgG with chromium atoms.

Animals↗
PubMed1978

II. Ultrastructural differences in attachment patterns of indicator cells to target cells in the rosette-forming process.

A comparison was made of the electron microscopic appearances of rosettes formed between mouse spleen lymphocytes and indicator cells with the ligands exposed on their surfaces either in clusters or in continuity. Sheep red blood cells (SRBC) coated with protein A of Staphylococcus aureus or human IgG by the chromium chloride technique were used as indicator cells with cluster distribution of the ligand. RBC coated with rabbit IgG anti-sheep red blood cells or staphylococci were used as indicator cells with uniform distribution of the ligand. Results show that the appearance of the zones of contact (point attachment or broad zone attachment) between lymphocytes and indicator cells are mainly influenced by the distribution of the specific ligands on the surface of the indicator cells and do not reflect the pattern of distribution of receptors on lymphocytes.

Animals↗
PubMed1975

Microautoradiographic study of cell proliferation in compensatory renal growth.

DNA synthesis in the renal parenchyma was studied by electron microscopic autoradiography 48 and 72 hours after unilateral nephrectomy in mice and weanling rats. The proportion of labelled nuclei belonging to the epithelium, endothelium, interstitial areas, or circulating cells was evaluated. Most of cells showing DNA synthesis were epithelial but many belonged to stroma. All the nephron segments were found to participate in compensatory hyperplasia, with a greater contribution, however, of the proximal convoluted tubules. DNA synthesis by the capillary endothelial cells occurred later than the peak epithelial mitotic activity. The site of DNA synthesis in nuclei was the euchromatin, the silver grains being uniformly distributed throughout the nuclear areas with condensed chromatin, and more seldom in the nuclear envelope areas or that of the perinucleolar satellites.

Animals↗
PubMed1972

Some ultrastructural aspects of endemic nephropathy of the Balkans.

Two renal biopsies selected from a large number of specimens taken by renal puncture from patients suffering from endemic nephropathy were studied by electron microscopy. Ultrastructural lesions were seen as electron-dense deposits in the walls of the glomerular capillaries, sometimes associated with glomerular proliferative processes. Such lesions, which are probably the early stages of this obscure renal disease, suggest that an auto-immune mechanism may be involved.

Humans↗